Quantitatively characterizing the microstructural features of breast ductal carcinoma tissues in different progression stages by Mueller matrix microscope

Abstract

Polarization imaging has been recognized as a potentially powerful technique for probing the microstructural information and optical properties of complex biological specimens. Recently, we have reported a Mueller matrix microscope by adding the polarization state generator and analyzer (PSG and PSA) to a commercial transmission-light microscope, and applied it to differentiate human liver and cervical cancerous tissues with fibrosis. In this paper, we apply the Mueller matrix microscope for quantitative detection of human breast ductal carcinoma samples at different stages. The Mueller matrix polar decomposition and transformation parameters of the breast ductal tissues in different regions and at different stages are calculated and analyzed. For more quantitative comparisons, several widely-used image texture feature parameters are also calculated to characterize the difference in the polarimetric images. The experimental results indicate that the Mueller matrix microscope and the polarization parameters can facilitate the quantitative detection of breast ductal carcinoma tissues at different stages.

Distinguishing structural features between Crohn's disease and gastrointestinal luminal tuberculosis using Mueller matrix derived parameters

“…Here as the examples, Figure A,B shows the imaging results of tissues from two individual CD patients, and Figure C,D shows the imaging results of tissues from two individual ITB patients. From Figure we can observe the structural features of the tissue samples as follows: (a) Compared with other elements, the m 24 , m 34 , m 42 and m 43 show some more prominent intensity distributions, which are mainly resulted from birefringence of the fibrous structures . The birefringent fibers surrounding the tuberculosis areas are induced by the inflammatory reactions.…”

“…The Mueller matrix derived parameters of clear associations with certain structural features have been demonstrated to be potentially useful for some diagnostic purposes . For instance, we preliminarily applied the Mueller matrix microscope and MMT parameters to the pathological detections and staging of human breast duct carcinoma , liver cancer and cirrhosis , thyroid cancer , skin cancer , cervical cancer , and so on . The results showed that the microscopic Mueller matrix derived parameters are powerful indicators to describe the structural features of different pathological samples quantitatively.…”

Quantitative Analysis of 4 × 4 Mueller Matrix Transformation Parameters for Biomedical Imaging

“…Recent applications of various cancerous tissues have demonstrated the diagnostic potential of Mueller matrix-derived parameters [6,8,17,23]. However, a quantitative analysis of the Mueller matrix parameters is still needed to help select a specific group of parameters suitable for a specific biomedical polarization measurement.…”

Detection of non‐small cell lung cancer cells based on microfluidic polarization microscopic image analysis

“…The application of Mueller matrix polarization decomposition and Mueller matrix transformation in the detection of polarization in biological tissues has obtained many important research results, such as human skin basal cell carcinoma, liver cancer, cervical cancer, colon cancer, and lung cancer . However, the popularity of this method is limited by the expensive polarized light microscopy based on Mueller matrix . The 3 × 3 Mueller matrix has the advantages of simple system components and wide requirements for light sources and high efficiency .…”