purpose: To (1) verify the reliability of the tibial tubercle-trochlear groove (TT-TG) distance measured by posterior condylar reference line (PCRL), surgical and anatomical transepicondylar axis (SEA and AEA) on MRI (TT-TGp, TT-TGs, and TT-TGa, respectively), (2) validate the diagnostic capacity of each parameter, (3) define the pathological threshold value of each TT-TG distance for tibial tubercle osteotomy (TTO).Methods: 86 patients with patellar dislocation (PD) and 86 healthy individuals were included. The intraclass correlation coefficient (ICC) was calculated to assess inter-observer reliability of each parameter. Unpaired t-test and Pearson correlation analysis were conducted. Receiver operating characteristic curves (ROC) and the areas under the curves (AUC) were analyzed to evaluate the diagnostic capacity of the parameters. The data of the control group was used to identify the pathological value of the parameters and Binary logistic regression model was established.Results: The TT-TGp, TT-TGs, and TT-TGa distance were greater in the study group (12.7±4.0, 12.0±4.1, and 11.9±4.2, respectively) than in the control group (8.2±2.9, 8.1±3.0, and 8.0±3.0, respectively) (P<0.001) and were correlated with trochlear dysplasia with a ρ of 0.47, 0.46, and 0.50, respectively (P<0.001). In the study group, the TT-TGp distance was greater than TT-TGs and TT-TGa distance (P<0.001). The ICC of each TT-TG distance was more than 0.85 in any degree of trochlear dysplasia. The AUC of the TT-TGp distance (0.811) was higher than the TT-TGs (0.777) and TT-TGa (0.768) distance. The pathological value of each TT-TG distance was 13.0mm. And the TT-TGp revealed a significant OR of 18.68 (95% CI [6.28, 55.50]) with regard to PD.Conclusion: Each TT-TG distance can be measured reliably by MRI and has a good diagnostic capacity for PD. TTO should be considered when a pathological TT-TG distance (>13.0 mm) is presented.Level of Evidence: Retrospective cohort study (diagnosis); Level of evidence, I.