Quantitative Ultrasound Assessment of Cartilage Degeneration in Ovariectomized Rats with Low Estrogen Levels

Wang, Qing; Liu, Zhiwei; Wang, Yinong; Pan, Qingya; Feng, Qianjin; Huang, Qinghua; Chen, Wufan

doi:10.1016/j.ultrasmedbio.2015.08.004

Cited by 7 publications

(6 citation statements)

References 31 publications

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“…Previous studies have reported that the characteristics of cartilage tissue could be evaluated using ultrasound . Compared with other imaging techniques such as X‐ray, computed tomography, and magnetic resonance imaging, ultrasound not only enables high‐resolution imaging and quantitative measurement of articular cartilage but it also has the advantage of low consumption and no radiation . In the current study, the ultrasound evaluation of in vivo results was consistent with morphological and molecular examination in vitro.…”

Section: Discussionsupporting

confidence: 81%

Diazoxide ameliorates severity of experimental osteoarthritis by activating autophagy via modulation of the osteoarthritis‐related biomarkers

Meng

Shen

et al. 2018

J of Cellular Biochemistry

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Accumulating evidence suggests that autophagy plays a protective role in chondrocytes and prevents cartilage degeneration in osteoarthritis (OA). The objective of this study was to investigate the effect of diazoxide on chondrocyte death and cartilage degeneration and to determine whether these effects are correlated to autophagy in experimental OA. In this study, a cellular OA model was established by stimulating SW1353 cells with interleukin 1β. A rat OA model was generated by transecting the anterior cruciate ligament combined with the resection of the medial menisci, followed by treatment with diazoxide or diazoxide combination with 3-methyladenine. The percentage of viable cells was evaluated using calcein-acetoxymethyl/propidium iodide double staining. The messenger RNA expression levels of collagen type II alpha 1 chain (COL2A1), matrix metalloproteinase 13 (MMP-13), TIMP metallopeptidase inhibitor 1 (TIMP-1), and a disintegrin and metalloproteinase with thrombospondin motifs 5 (ADAMTS5) were determined using quantitative real-time polymerase chain reaction. The cartilage thickness and joint space were evaluated using ultrasound. SW1353 cell degeneration and autophagosomes were observed using transmission electron microscopy. The expression levels of microtubule-associated protein 1 light chain 3 (LC3), beclin-1, P62, COL2A1, and MMP-13 were evaluated using immunofluorescence staining and Western blot analysis. Diazoxide significantly attenuated articular cartilage degeneration and SW1353 cell death in experimental OA. The restoration of autophagy was observed in the diazoxide-treated group. The beneficial effects of diazoxide were markedly blocked by 3-methyladenine. Diazoxide treatment also modulated the expression levels of OA-related biomarkers. These results demonstrated that diazoxide exerted a chondroprotective effect and attenuated cartilage degeneration by restoring autophagy via modulation of OA-related biomarkers in experimental OA. Diazoxide treatment might be a promising therapeutic approach to prevent the development of OA.

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Section: Discussionsupporting

confidence: 81%

Diazoxide ameliorates severity of experimental osteoarthritis by activating autophagy via modulation of the osteoarthritis‐related biomarkers

Meng

Shen

et al. 2018

J of Cellular Biochemistry

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“…Quantitative local measurements of cartilage surface roughness have been performed with other imaging modalities, including optical coherence tomography 16 , scanning electron microscopy 17 , and atomic force microscopy 17 , but these local measures are useful only in small areas in relation to the entire surface of a rat tibia and are usually destructive techniques. Ultrasound has shown some utility in detecting changes in cartilage surface roughness in cartilage plugs 18e22 , intact rabbit joints 23 , and ex vivo rat joints 24 . However, ultrasound measurements do not provide measurements of other hallmarks of OA progression such as proteoglycan loss, subchondral bone sclerosis, or osteophyte development.…”

Section: Introductionmentioning

confidence: 99%

Contrast enhanced μCT imaging of early articular changes in a pre-clinical model of osteoarthritis

Reece

Thote

Lin

et al. 2018

Osteoarthritis and Cartilage

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“…In the same study, the URI increased significantly with enzymatic digestion with collagenase but not trypsin and chondroitinase ABC. Wang et al 39 reported significant URI increases in condylar and tibial plateau cartilage of rats after ovariectomy. In vivo , Kaleva et al 32 found that URI measured arthroscopically could differentiate between intact and fibrillated cartilage.…”

Section: Discussionmentioning

confidence: 99%

“… 28 , 31 , 32 The URI is a quantitative measure of the cartilage surface roughness and can describe morphological changes of the surface. 34 , 35 Several ex vivo studies used the URI to assess cartilage degeneration in OA, 35 - 37 mechanical or enzymatic degradation, 34 following acute injury, 38 with ovariectomy 39 or after sliding shear. 40 Furthermore, it was shown that the URI increases with deteriorating cartilage and is correlated with OA grade and histological scoring.…”

Section: Introductionmentioning

confidence: 99%

Ultrasound-Based Quantification of Cartilage Damage After In Vivo Articulation With Metal Implants

et al. 2021

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Objective This study aims to evaluate the applicability of the ultrasound roughness index (URI) for quantitative assessment of cartilage quality ex vivo (post-mortem), after 6 months of in vivo articulation with a Focal Knee Resurfacing Implant (FKRI). Design Goats received a metal FKRI ( n = 8) or sham surgery ( n = 8) in the medial femoral condyles. After 6 months animals were sacrificed, tibial plateaus were stained with Indian ink, and macroscopic scoring of the plateaus was performed based on the ink staining. The URI was calculated from high-frequency ultrasound images at several sections, covering both areas that articulated with the implant and non-articulating areas. Cartilage quality at the most damaged medial location was evaluated with a Modified Mankin Score (MMS). Results The URI was significantly higher in the FKRI-articulating than in the sham plateaus at medial articulating sections, but not at sections that were not in direct contact with the implant, for example, under the meniscus. The mean macroscopic score and MMS were significantly higher in the FKRI-articulating group than in the sham group ([Formula: see text], [Formula: see text], respectively). Correlation coefficients between URI and macroscopic score were significant in medial areas that articulated with the implant. A significant correlation between URI and MMS was found at the most damaged medial location ([Formula: see text]). Conclusions This study demonstrates the potential of URI to evaluate cartilage roughness and altered surface morphology after in vivo articulation with a metal FKRI, rendering it a promising future tool for quantitative follow-up assessment of cartilage quality.

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Quantitative Ultrasound Assessment of Cartilage Degeneration in Ovariectomized Rats with Low Estrogen Levels

Cited by 7 publications

References 31 publications

Diazoxide ameliorates severity of experimental osteoarthritis by activating autophagy via modulation of the osteoarthritis‐related biomarkers

Diazoxide ameliorates severity of experimental osteoarthritis by activating autophagy via modulation of the osteoarthritis‐related biomarkers

Contrast enhanced μCT imaging of early articular changes in a pre-clinical model of osteoarthritis

Ultrasound-Based Quantification of Cartilage Damage After In Vivo Articulation With Metal Implants

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