Quantitative Ultramorphological Analysis of Human Sperm: Diagnosis and Management of Male Infertility

Bartoov, B.; Eltes, F.; Reichart, M.; Langzam, J.; Lederman, H.; Zabludovsky, N.

doi:10.1080/014850199262823

Cited by 10 publications

(7 citation statements)

References 39 publications

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“…Indeed, the following sperm nuclear parameters were found to be related to pregnancy achievement: ultrastructural examination of nuclear shape and chromatin texture [18], chromatin decondensation induced in vitro [19], sperm chromatin packaging quality using chromomycin A3 [20] or aniline blue staining [21], DNA staining after in situ denaturation of chromatin [22], in situ nick translation [20], TUNEL [23], and oxidized nucleoside (8-hydroxy-2Ј-deoxyguanosine) content in total DNA [24].…”

Section: Introductionmentioning

confidence: 99%

In Vivo and In Vitro Impairment of Human and Ram Sperm Nuclear Chromatin Integrity by Sexually Transmitted Ureaplasma urealyticum Infection1

Reichart

Kahane

Bartoov

2000

Biology of Reproduction

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The incidence of Ureaplasma urealyticum infection in the semen of infertile men is variable (7%-42%). Evidence has accumulated through routine semen analysis to suggest that this infection can cause embryo loss without necessarily affecting sperm quality. The aim of this study was to specifically investigate the effects of U. urealyticum infection on sperm chromatin stability and DNA integrity, which are known to be correlated to pregnancy outcome. Sperm cells isolated from human semen infected in vivo with U. urealyticum exhibited a low percentage of stable chromatin as determined by nuclear chromatin decondensation assay (42% +/- 4.8%, n = 8) and a high percent of denatured DNA as determined by sperm chromatin structure assay (60.9% +/- 9.1%, n = 7). After doxycyclin treatment, a significant improvement in both parameters was observed (73.7% +/- 3.6%, P: < 0.001 and 30.1% +/- 3.5%, P: < 0.008, respectively). Sperm cells infected in vitro exhibited higher rates of viability and motility than uninfected cells. In contradistinction, U. urealyticum caused significant dose- and time-dependent chromatin decondensation and DNA damage. The percentage of human sperm cells with denatured DNA increased significantly by 54.9% +/- 23.9% and 47. 9% +/- 12.1%, after 30 min infection with serotypes 8 and 3, respectively, at a multiplicity of infection of 100 ureaplasmas per sperm compared with uninfected control cells. The damage to DNA was significantly more pronounced in infected ram sperm (180.9% +/- 21. 5%). These results indicate that preserved sperm activity post U. urealyticum infection resulted in damage to paternal DNA, although a high fertilization rate was maintained, and embryonic development may, therefore, be impaired.

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Section: Introductionmentioning

confidence: 99%

In Vivo and In Vitro Impairment of Human and Ram Sperm Nuclear Chromatin Integrity by Sexually Transmitted Ureaplasma urealyticum Infection1

Reichart

Kahane

Bartoov

2000

Biology of Reproduction

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“…Electron microscopy of sperm is not a new assay by any means, but its use in the evaluation of infertility continues to evolve [1,5]. It remains the gold standard for the evaluation of ultrastructure defects such as acrosome morphology, sperm tail defects due to microtubule rearrangements, and sperm centriole morphology [51].…”

Section: Assays Of General Biochemistry and Ultrastructurementioning

confidence: 99%

Semen Analysis at the Turn of the Century: An Evaluation of Potential Uses of New Sperm Function Assays

Carrell¹

2000

Archives of Andrology

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“…Transmission electron microscopy enabled the evaluation of nucleus, acrosome, tail or other elements that cannot be studied with light microscopy. Different classification of ultrastructural spermatozoa defects were taken into consideration by various authors [Bartoov et al 1999;Kupker et al 1998;Zamboni 1992].…”

Section: Resultsmentioning

confidence: 99%

“…An in-depth evaluation of semen quality by transmission electron microscopy (TEM) can improve the diagnosis of male sub fertility and can give substantial information about the fertilizing competence of male gamete [Kupker et al 1998, Jian-Jun andYue-Min 2004;Takeuchi et al 2004]. It has been reported that normal spermatozoa ultrastructure correlated with positive IVF results [Mashiach et al 1992;Zamboni 1991;Baccetti et al 1995;Carbone et al 1998;Bartoov et al 1999]. TEM evaluation of sperm can also identify potentially inheritable genetic disorders (e.g., primary ciliary dyskinesia, Kartagener's syndrome), providing valuable information for couples contemplating ICSI [Carbone et al 1998;Lamb 1999].…”

Section: Introductionmentioning

confidence: 99%

Human Spermatozoa Ultrastructure Assessment in the Infertility Treatment by Assisted Reproduction Technique

Kotwicka

Depa-Martynów

Butowska

et al. 2007

Archives of Andrology

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The aim of this study was to prospectively investigate the spermatozoa ultrastructure in relation to the results of in vitro fertilization -embryo transer (IVF-ET).Forty-nine consecutive couples admitted for IVF-ET were prospectively evaluated for electron microscopic spermatozoa morphology and the outcome of IVF-ET. Thirty-four couples revealed successful fertilization, defined as presence of two pronuclei 14-16 hours after spermatozoa administration, while the remaining 15 formed the failure group. Spermatozoa fixed with 2.5% glutaraldehyd and embedded in Spurr's resin were analyzed with JAM 100 S transmission electron microscope (TEM) for the following ultrastructure abnormalities: head deformity, cytoplasmic residues, chromatin condensation failures, acrosomal alterations, neck defects, midpiece defects, principal piece and end-piece defects and immature forms.Successful IVF-ET couples revealed a significantly higher percentage of normal spermatozoa utrastructure (32.0 AE 13.1% versus 17.1 AE 13.4%, p < 0.001). Failed IVF-ET couples represented a significantly higher percentage of chromatin condensation failures (9.8 AE 5.1% versus 5.7 AE 5.3%, p < 0.05) and tail defects (16.7 AE 11.5% versus 7.2 AE 7.2%, p < 0.001). A positive correlation between normal ultrastructure spermatozoa percentage and fertilized oocytes percentage was found (r ¼ 0.35, p < 0.05).Our data suggest that spermatozoa TEM findings correlate with IVF-ET results. Ultrastructural estimation of spermatozoa can improve the diagnosis of male fertility and may explain some reasons of failure in assisted reproduction methods. We consider systematic TEM spermatozoa examination in cases with failed IVF-ET prior to intracytoplasmic sperm injection (ICSI). KEYWORDS in vitro fertilization, spermatozoa, ultrastructureAbbreviations: IVF-ET: in vitro fertilization -embryo transfer; TEM: transmission electron microscope; ICSI intracytoplasmic sperm injection.

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Quantitative Ultramorphological Analysis of Human Sperm: Diagnosis and Management of Male Infertility

Cited by 10 publications

References 39 publications

In Vivo and In Vitro Impairment of Human and Ram Sperm Nuclear Chromatin Integrity by Sexually Transmitted Ureaplasma urealyticum Infection1

In Vivo and In Vitro Impairment of Human and Ram Sperm Nuclear Chromatin Integrity by Sexually Transmitted Ureaplasma urealyticum Infection1

Semen Analysis at the Turn of the Century: An Evaluation of Potential Uses of New Sperm Function Assays

Human Spermatozoa Ultrastructure Assessment in the Infertility Treatment by Assisted Reproduction Technique

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