Quantitative ubiquitylomics reveals the ubiquitination regulation landscape in oral adenoid cystic carcinoma

Abstract

Adenoid cystic carcinoma (ACC) is an extremely rare salivary gland tumor with a poor prognosis and needs attention on molecular mechanisms. Protein ubiquitination is an evolutionarily conserved post-translational modification (PTM) for substrates degradation and control diverse cellular functions. The board cellular function of ubiquitination network holds great promise to detect potential targets and identify respective receptors. Novel technologies are discovered for in-depth research and characterization the precise and dynamic regulation of ubiquitylomics in multiple cellular processes during cancer initiation, progression and treatment. In this study, 4D label free quantitative techniques of ubiquitination proteomics were used and we identified a total of 4152 ubiquitination sites in 1993 proteins. We also performed a systematic bioinformatics analysis for differential modified proteins and peptides containing quantitative information through the comparation between OACC tumor with adjacent normal tissues, as well as the identification of 8 protein clusters with motif analysis. Our findings offered an important reference of potential biomarkers and effective therapeutic targets for ACC.

Analysis of proteome and post-translational modifications of 2-hydroxyisobutyrylation reveals the glycolysis pathway in oral adenoid cystic carcinoma

“…After being instantaneously snap-frozen in liquid nitrogen, the tissues were kept at − 80 °C. The analysis of the proteome [ 14 ] and quantitative ubiquitylomics of post-translational modifications of ubiquitination [ 15 ] in OACC were performed by using these same samples.…”

Advances in the application of label‐free quantitative proteomics techniques in malignancy research

“…Salivary gland tumor is a common oral and maxillofacial tumor, of which adenoid cystic carcinoma is a rare type with slow growth, high invasiveness, and ease of metastasis. To further study its molecular mechanism, Li, Wang, et al (2021) 3.2 | Malignant tumors of the digestive system…”

“…Salivary gland tumor is a common oral and maxillofacial tumor, of which adenoid cystic carcinoma is a rare type with slow growth, high invasiveness, and ease of metastasis. To further study its molecular mechanism, Li, Wang, et al (2021) used 4D‐label‐free ubiquitination proteomics quantitative technology to analyze oral adenoid cystic carcinoma tumors and adjacent normal tissues, identified 4152 ubiquitination sites, obtained 8 protein clusters by analyzing the function of these proteins, and found the role of cytoskeletal ubiquitination and extracellular matrix–related proteins in the occurrence and metastasis of oral adenoid cystic carcinoma can work. These findings can be used as a reference in the development of therapeutic targets for salivary gland tumors.…”

Comparative proteomic and clinicopathological analysis of breast adenoid cystic carcinoma and basal-like triple-negative breast cancer

“…The quantitative proteomic analysis is a useful complementary analysis to genomic and transcriptomic analyses for it provides additional biological information that would have been impossible to get merely by genomics approaches ( 18 ). Proteomic analysis has been demonstrated as a useful tool with the potential to identify cancer biomarkers and find new therapeutic targets in salivary gland ACC, whereas the detailed information on breast ACC is unknown ( 19 – 21 ).…”