Quantitative tyramine analysis method for <i>Apis mellifera</i> larvae infected with <i>Melissococcus plutonius</i>, the causative agent of European foulbrood
Daisuke TAKAMATSU,
Ryuichi UEGAKI,
Mariko OKAMOTO
et al.
Abstract:Tyramine, a trace monoamine produced from tyrosine by decarboxylation and found naturally in foods, plants, and animals, is a suspected virulence factor of Melissococcus plutonius that causes European foulbrood in honey bee brood. In the present study, we developed a method for quantitative analysis of tyramine in culture medium and honey bee larvae with a limit of quantitation of 3 ng/mL and a recovery rate of >97% using Liquid Chromatography-Mass Spectrometry/Mass Spectrometry and deuteriumlabeled tyramine, … Show more
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