Quantitative Trait Loci that Control Body Weight and Obesity in an F2 Intercross between C57BL/6J and DDD.Cg-Ay Mice

The Journal of Veterinary Medical Science

2013

Self Cite

ABSTRACT. DDD.Cg-A y female mice developed massive obesity as compared with B6.Cg-A y female mice. We previously identified quantitative trait loci (QTLs) for obesity on chromosomes 1, 6, 9 and 17 in F 2 female mice, including F 2 A y (F 2 mice with the A y allele) and F 2 non-A y mice (F 2 mice without the A y allele), produced by crossing C57BL/6J and DDD.Cg-A y strains. We here addressed the question whether the obesity QTLs share genetic bases with putative QTLs for plasma glucose, insulin and leptin concentrations. We performed QTL analyses for the first principal component (PC1) extracted from these metabolic measurements to identify the genes that contributed to the comprehensive evaluation of metabolic traits. By single QTL scans, we identified two significant QTLs for insulin concentration on chromosomes 6 and 12, three for leptin concentration on chromosomes 1, 6 and 17, and five for PC1 on chromosomes 1, 6, 12 (two loci) and 17. Although insulin and leptin concentrations and PC1 were not normally distributed in combined F 2 mice, results of single QTL scans by parametric and non-parametric methods were very similar. Therefore, QTL scan by the parametric method was performed with the agouti locus genotype as a covariate. A significant QTL × covariate interaction was found for PC1 on chromosome 9. All obesity QTLs had significant metabolic effects. Thus, obesity-and diabetes-related traits in DDD.Cg-A y mice were largely controlled by QTLs on chromosomes 1, 6, 9, 12 and 17.

Section: Comparison Of Plasma Glucose and Leptin Concentrations Amongsupporting

confidence: 91%

Section: Micementioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

QTL Mapping of Genes Controlling Plasma Insulin and Leptin Concentrations: Metabolic Effect of Obesity QTLs Identified in an F2 Intercross between C57BL/6J and DDD.Cg-Ay Inbred Mice

The Journal of Veterinary Medical Science

2013

Self Cite

“…The congenic mouse B6.Cg-A y (hereafter B6-A y ) strain was purchased from the Jackson Laboratory (Bar Harbor, ME, U.S.A.). The process of establishing the DDD-A y strain has been described previously [21]. Hereafter, DDD-A y and B6-A y are referred together as "A y mice".…”

Section: Micementioning

confidence: 99%

“…QTL analyses for body weight and obesity (defined by body mass index) was performed in F 2 mice produced by crossing C57BL/6J (B6) females and DDD-A y males [21]. Four significant QTLs for body weight were identified on chromosomes 6, 9, and 17 (two loci), and four significant QTLs for obesity were identified on chromosomes 1, 6, 9, and 17.…”

mentioning

confidence: 99%

Quantitative Trait Loci that Control Plasma Lipid Levels in an F2 Intercross between C57BL/6J and DDD.Cg-Ay Inbred Mouse Strains

The Journal of Veterinary Medical Science

2012

Self Cite

ABSTRACT. The objectives of this study were to characterize plasma lipid phenotypes and dissect the genetic basis of plasma lipid levels in an obese DDD.Cg-A y mouse strain. Plasma triglyceride (TG) levels were significantly higher in the DDD.Cg-A y strain than in the B6.Cg-A y strain. In contrast, plasma total-cholesterol (CHO) levels did not substantially differ between the two strains. As a rule, the A y allele significantly increased TG levels, but did not increase CHO levels. Quantitative trait locus (QTL) analyses for plasma TG and CHO levels were performed in two types of F 2 female mice [F 2 A y (F 2 mice carrying the A y allele) and F 2 non-A y mice (F 2 mice without the A y allele)] produced by crossing C57BL/6J females and DDD.Cg-A y males. Single QTL scan identified one significant QTL for TG levels on chromosome 1, and two significant QTLs for CHO levels on chromosomes 1 and 8. When the marker nearest to the QTL on chromosome 1 was used as covariates, four additional significant QTLs for CHO levels were identified on chromosomes 5, 6, and 17 (two loci). In contrast, consideration of the agouti locus genotype as covariates did not detect additional QTLs. DDD.Cg-A y showed a low CHO level, although it had Apoa2 b , which was a CHO-increasing allele at the Apoa2 locus. This may have been partly due to the presence of multiple QTLs, which were associated with decreased CHO levels, on chromosome 8. KEY WORDS: cholesterol, mice, plasma lipid levels, quantitative trait locus (QTL), triglyceride.

Quantitative trait loci that control body weight in DDD/Sgn and C57BL/6J inbred mice

Kojima

2016

Mamm Genome

Inbred DDD/Sgn mice are heavier than inbred C57BL/6J mice. In the present study, we performed quantitative trait loci (QTL) mapping for body weight using R/qtl in reciprocal F male populations between the two strains. We identified four significant QTL on Chrs 1, 2, 5, and 17 (proximal region). The DDD/Sgn allele was associated with increased body weight at QTL on Chrs 1 and 5, and the DDD/Sgn allele was associated with decreased body weight at QTL on Chrs 2 and 17. A multiple regression analysis indicated that the detected QTL explain 30.94 % of the body weight variation. Because DDD/Sgn male mice have extremely high levels of circulating testosterone relative to other inbred mouse strains, we performed QTL mapping for plasma testosterone level to examine the effect of testosterone levels on body weight. We identified one suggestive QTL on Chr 5, which overlapped with body weight QTL. The DDD/Sgn allele was associated with increased testosterone level. Thus, we confirmed that there was a genetic basis for the changes in body weight and testosterone levels in male mice. These findings provide insights into the genetic mechanism by which body weight is controlled in male mice.