Quantitative trait loci identified for foliar phylloxera resistance in a hybrid grape population

Abstract

Background and Aims: A hybrid grapevine biparental mapping population was evaluated for resistance to grape phylloxera (Daktulosphaira vitifoliae Fitch) in the greenhouse and in the field. The family (n = 125) was derived from a cross of two University of Minnesota breeding selections from a complex pedigree with multiple Vitis species. Methods and Results: The vines were evaluated for foliar phylloxera severity using visual rating in the field for multiple years. In the greenhouse, a replicated experimental design was used to quantify phylloxera severity using different traits including number of galls per plant, area under the disease progress curve, proportion of leaves with galls, average number of galls per leaf and visual rating. The plants used in the greenhouse experiment were also visually rated for root infestation. Conclusions: Quantitative trait loci (QTL) mapping for the foliar resistance traits (reduced severity) routinely identified the region spanning~10 to 30 cM on linkage group (LG) 14 to be associated with resistance, and inherited from the mother parent MN1264. The plants used in the greenhouse experiment were also visually rated for root infestation, and subsequent QTL mapping identified regions on LG 5 of the maternal map and LG 10 of the paternal map (MN1246) associated with root resistance.Significance of the Study: The new QTLs identified in this report are potential novel sources of resistance and the first report of QTL for foliar phylloxera resistance in a hybrid grapegrowing region of North America. Molecular markers linked to resistance can be utilised in marker-assisted breeding.

Characterization of genetic determinants of the resistance to phylloxera, Daktulosphaira vitifoliae, and the dagger nematode Xiphinema index from muscadine background

“…'C2-50'(Smith et al 2018). Two other QTLs have been detected on LG 5 and LG 10 in an F1 cross containing at least six Vitis species in the ancestry(Clark et al 2018). In our study, three new QTLs were identified from M. rotundifolia background.…”

“…A leaf-specific phylloxera resistance locus overlapped the location of RDV2 on LG 14 in a F1 family from a cross containing at least six Vitis species in the ancestry (V. vinifera, V. riparia, V. rupestris, V. labrusca, V. aestivalis and V. berlandieri). In contrast, from the same plant material, a locus for resistance to the root form of phylloxera mapped to chromosomes 5 and 10 (Clark et al 2018).…”

Construction of a High-Density Genetic Map and Mapping of Firmness in Grapes (Vitis vinifera L.) Based on Whole-Genome Resequencing

“…In the past ten years, a number of grape genetic maps have been constructed based on different mapping populations [20,[22][23][24][25][26][27][28][29][30][31][32][33]. Numerous QTLs for important economic traits, including resistance to downy mildew [10,16,22,[34][35][36][37][38], powdery mildew [34,36,[39][40][41], anthracnose [24], root-knot nematodes [42], and grape phylloxera [43,44], as well as flower sex [45,46], berry color [46,47], seedlessness [48], berry weight [18,32], soluble solid content [32], acidity [20], and muscat flavor [49], have been identified.…”

Novel stable QTLs identification for berry quality traits based on high-density genetic linkage map construction in table grape

“…Numerous QTLs for grapevine relevant traits, including berry weight and size [3][4][5][6], sweetness and acids [7,8], seedlessness [4,5,9,10], disease-resistance traits [11][12][13][14][15], and so on, have been mapped. In this decade, consumers pay more and more attention on grape quality, not only the taste (flavor, texture and so on) but also the appearance traits (color, berry size and shape).…”