Quantitative Trait Loci Associated with Resistance to Empoasca in Common Bean

Abstract

Empoasca species leafhoppers are a major insect pest of common bean (Phaseolus vulgaris L.), that cause significant economic losses in both tropical (E. kraemeri) and temperate (E. fabae) regions of the Americas. The objective of this study was to use Insertion–Deletion (InDel) and single‐nucleotide polymorphism (SNP) markers from the BARCBean6K_3 Beadchip to identify quantitative trait loci (QTL) associated with traits related to leafhopper resistance in common bean. Traits for leaf curl and leaf burn damage, as well as Empoasca spp. nymph counts, were evaluated in an inbred backcross line population (Matterhorn*/EMP507) of beans in temperate (Michigan) and tropical (Puerto Rico) climates. Fourteen QTL associated with resistance to E. fabae and E. kraemeri were identified on five chromosomes explaining up to 66.0% of the phenotypic variation for single resistance traits. A major QTL cluster associated with multiple resistance traits and closely linked to the P color gene was detected for both leafhopper species in multiple seasons under both choice and no‐choice treatments on Pv07 (LH7.1, LH7.2, LH7.3), thus validating a similar QTL identified in previous studies. A novel QTL (LH2.2) for E. fabae nymph counts, identified on Pv02 in three seasons, may be associated with antibiosis resistance. Resistance to each leafhopper species appears to be controlled by separate genetic mechanisms in common bean as there was little overlap of QTL regions between species. These QTL could be used to develop beans with leafhopper resistance as an alternative to costly chemical controls while reducing risks to the environment and human health.

Mapping Fusarium solani and Aphanomyces euteiches Root Rot Resistance and Root Architecture Quantitative Trait Loci in Common Bean

“…In a study on drought tolerance in dry bean, Mukeshimana et al (2014) constructed a linkage map of 1351 cM in length using 2122 SNP markers. Brisco et al (2014) used SNP markers in common bean to detect QTL for Empoasca resistance. Felicetti et al (2012) used SNPs to localize the slow darkening ( sd ) gene on Pv07.…”

Registration of TARS‐LH1 pinto bean germplasm with resistance to the leafhopper pest

“…The plant symptom that results is termed "hopper burn" and is further classified into leaf curl (LC) and leaf burn (LB) traits (Backus, Serano, & Ranger, 2005;Murray, Michaels, Pauls, & Schaafsma, 2001). In addition, differences in numbers of flightless nymphs on the underside of leaves have been associated with resistance and may be an antibiosis response to E. fabae (Brisco, Porch, Cregan, & Kelly, 2014). However, seed yield under leafhopper pressure in the field is the most reliable measure of resistance (Kornegay & Cardona, 1990).…”

“…suggested quantitative genetic control (Galwey & Evans, 1982); independent genetic control of LB and LC traits (Murray et al, 2001); additive and dominant control of yield, LB, and LC traits; and additive genetic control of nymph numbers (Kornegay & Temple, 1986). Molecular genetic studies have found LB and LC quantitative trait loci (QTL) clustered on Pv07 near the P locus in populations with resistance derived from EMP 507 (Brisco et al, 2014) and EMP 419 (Murray, Michaels, Cardona, Schaafsma, & Pauls, 2004). Differences in location of other LB and LC QTL between germplasm lines resistant to E. kraemeri and E. fabae indicate additional loci controlling these traits (Brisco et al, 2014).…”

“…Molecular genetic studies have found LB and LC quantitative trait loci (QTL) clustered on Pv07 near the P locus in populations with resistance derived from EMP 507 (Brisco et al, 2014) and EMP 419 (Murray, Michaels, Cardona, Schaafsma, & Pauls, 2004). Differences in location of other LB and LC QTL between germplasm lines resistant to E. kraemeri and E. fabae indicate additional loci controlling these traits (Brisco et al, 2014). One LC QTL was identified on Pv01 for E. fabae in EMP 419 (Murray et al, 2004).…”

“…This genomic location may be associated with the determinate Fin locus because the indeterminate plant habit can result in plant recovery after leafhopper infestation (Murray et al, 2001). The first E. fabae nymph count QTL in bean was found on Pv02, which may be associated with an antibiosis response conferred by the EMP 507 genotype (Brisco et al, 2014). Color traits, including the P locus conditioning white seed in common bean near the Pv07 QTL mentioned above, and leaf color may have roles in this antixenosis response (Brisco, 2012).…”