Quantitative Trait Loci Associated with Resistance to Rice Stripe Virus and Small Brown Planthopper Infestation in Rice

Abstract

Rice stripe is a serious viral disease of japonica rice (Oryza sativa L.) cultivars in Asia. Resistance to this disease can be divided into that to rice stripe virus (RSV) and to the pest (small brown planthopper, SBPH). Here we have used a doubled‐haploid (DH) population bred from the cross Zhaiyeqing8 (ZYQ8) × Jingxi17 (JX17) and four separate inoculation methods to genetically dissect the rice stripe resistance present in ZYQ8. Two linked RSV resistance quantitative trait loci (QTLs) were located on chromosome 1, and one on chromosome 11, jointly explaining 30 to 44% of the trait variance. The main‐effect qSTV11 was mapped close to the established RSV resistance gene Stvb‐i For resistance to SBPH, three QTLs were located, mapping to chromosomes 1, 2, and 11, and jointly explaining 44 to 51% of the trait variance. The large‐effect qSBPH1 appears to be a novel QTL conferring a degree of tolerance and antibiosis against SBPH. The positive alleles at all six QTLs were present in ZYQ8. Analysis of a set of chromosome segment substitution lines (CSSLs) in which chromosomal segments of various indica varieties were present in a japonica background showed that rice stripe resistance genes/QTLs are common on the long arm of chromosome 1.

Detection of Quantitative Trait Loci (QTLs) for Resistances to Small Brown Planthopper and Rice Stripe Virus in Rice Using Recombinant Inbred Lines

“…On the other hand, Zhang et al ., Duan et al and Le et al . also detected two stable QTL on chromosome 11 and 12 using different mapping populations [25–27]. Comparing QTLs for SBPH resistance identified in this study with those already reported in the literature, it seems that the QTL against SBPH detected in this study are novel.…”

Mapping of quantitative trait loci associated with rice black‐streaked dwarf virus disease and its insect vector in rice (Oryza sativa L.)

“…Nowadays, for controlling this disease, insecticides have been widely used to control its transmitting vector SBPH, but the effectiveness is undesirable, and simultaneously caused great harm on the environment. Therefore, identification and utilization of insect and disease resistance genes are considered to be the most effective and economical measure (Zhang et al., ). In this study, we found that a rice variety WR24 displayed high resistance to both RBSDVD and SBPH, indicating that WR24 is a valuable germplasm line for development of resistant cultivars.…”

“…Small brown planthopper transmits virus disease (such as RBSDVD and RSV) and causes a direct damage to the plant. In previous studies, more QTLs against SBPH have been mapped using several populations (Duan et al., , ; Duan, Wan et al., ; Duan, Zhang et al., ; Sun et al., ; Tuyen et al., 2012; Wang et al., ; Zhang et al., ). Compared with these QTLs, qSBPH7 WR24 on chromosome 7 was located in the same interval as qSBPH7 .…”