Quantitative trait loci analysis reveals a correlation between the ratio of sucrose/raffinose family oligosaccharides and seed vigour in <i>Medicago truncatula</i>

Vandecasteele, Céline; Teulat, Béatrice; Paven, Marie–Christine Morère-Le; Leprince, Olivier; Vu, Benoît Ly; Viau, Laure; Ledroit, Lydie; Pelletier, Sandra; Payet, N.; Satour, Pascale; Lebras, Camille; Gallardo, Karine; Huguet, Thierry; Limami, Anis M.; Prospéri, Jean-Marie; Buitink, Julia

doi:10.1111/j.1365-3040.2011.02346.x

Cited by 74 publications

(93 citation statements)

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“…3 and 4). In M. truncatula species, this relationship, characterized by a very high R 2 coefficient (≥0.96), has been established in both maturing seeds of Jemalong, harvested during longevity acquisition, and full mature seeds of Jemalong and DZA315-16, two genotypes of naturally contrasting seed quality (34). Thus, MSR capacity appears to be a good marker to predict seed performance in this species.…”

Section: Discussionmentioning

confidence: 94%

“…After characterizing the MSR family in this species, we investigated MSR abundance and activity in seeds of varying quality, using artificial aging treatments and taking advantage of the natural variability reported for this trait (34). We then analyzed the longevity of seeds in Arabidopsis thaliana lines, which exhibit altered MSR capacity owing to genetic transformation.…”

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confidence: 99%

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Evidence for participation of the methionine sulfoxide reductase repair system in plant seed longevity

Châtelain

Satour

Laugier

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

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102

103

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Seeds are in a natural oxidative context leading to protein oxidation. Although inevitable for proper progression from maturation to germination, protein oxidation at high levels is detrimental and associated with seed aging. Oxidation of methionine to methionine sulfoxide is a common form of damage observed during aging in all organisms. This damage is reversible through the action of methionine sulfoxide reductases (MSRs), which play key roles in lifespan control in yeast and animal cells. To investigate the relationship between MSR capacity and longevity in plant seeds, we first used two Medicago truncatula genotypes with contrasting seed quality. After characterizing the MSR family in this species, we analyzed gene expression and enzymatic activity in immature and mature seeds exhibiting distinct quality levels. We found a very strong correlation between the initial MSR capacities in different lots of mature seeds of the two genotypes and the time to a drop in viability to 50% after controlled deterioration. We then analyzed seed longevity in Arabidopsis thaliana lines, in which MSR gene expression has been genetically altered, and observed a positive correlation between MSR capacity and longevity in these seeds as well. Based on our data, we propose that the MSR repair system plays a decisive role in the establishment and preservation of longevity in plant seeds.seed deterioration | seed viability | redox systems | antioxidant enzymes S uccessful crop implantation is crucial for maximal plant yield and relies on seed physiological quality, which is acquired at the end of development, during maturation. Quality is expressed by the capacity of seeds to germinate in a fast and homogenous manner before and after storage (seed longevity) and to provide proper seedling establishment in various environments (seed vigor). Quality is controlled by genetic traits and varies greatly among species and cultivars. The establishment of quality also depends on the prevailing environmental conditions during seed development and storage (1, 2).During the maturation phase, mechanisms are put in place to prevent and repair desiccation damage, as well as to allow survival of seeds in the dry state (3-5). Nonetheless, drying of seeds on mother plants can occur prematurely in drought conditions, leading to low-quality seeds (6-8). Thus, in the context of a warming climate, producing good-quality seeds represents a global challenge to sustain current yield levels (9, 10).The mechanisms underlying seed quality are controlled in part by abscisic acid (ABA), the phytohormone involved in plant responses to dehydration that is produced during seed maturation. Of note, ABA triggers the accumulation of late-embryogenesis abundant proteins, heat-shock proteins (HSPs), and storage proteins (11). Late-embryogenesis abundant proteins and HSPs are thought to primarily protect cell structures when water is removed. Reserve proteins, through their sensitivity to reactive oxygen species (ROS), play a determinant role in limiting oxidative damage...

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Section: Discussionmentioning

confidence: 94%

mentioning

confidence: 99%

Evidence for participation of the methionine sulfoxide reductase repair system in plant seed longevity

Châtelain

Satour

Laugier

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

102

103

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“…Another characteristic that can be determined genetically is the chemical composition of the seeds, such as storage protein, starch and soluble sugar contents (Bewley and Black, 1994;Song and Messing, 2003;Vandecasteele et al, 2011;Wu and Messing, 2014), and these can also influence seed vigour (Han et al, 2014). The mature maize endosperm is a major storage site for starch and protein, valuable storage reserves for the establishing seedling.…”

Section: Resultsmentioning

confidence: 99%

“…Thus the performance of hybrid seeds changes according to the selected genotypes, and differences between reciprocal crosses exist (Navratil and Burris, 1984;Roveri-José et al, 2004). This manifestation of phenotype may be attributable to seed chemical composition, especially regarding the quantity, identity and mobilization of storage proteins, lipids, starch and soluble sugars, as these can influence seed physiological potential (Footitt et al, 2002;McDonough et al, 2004;Vandecasteele et al, 2011), including quality (Wang and Frei, 2011).…”

Section: Introductionmentioning

confidence: 99%

Reciprocal effect of parental lines on the physiological potential and seed composition of corn hybrid seeds

et al. 2017

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Obtaining corn hybrid seeds (Zea mays L.) with high vigour depends on the parental lines and the direction of the cross, and this relates to seed desiccation tolerance and composition. This research studied reciprocal crosses between pairs of proprietary, elite parent lines (L1 and L5; L2 and L4) producing hybrid seeds with different qualities attempting to correlate vigour with seed composition, focusing on storage proteins, starch and soluble sugar amounts. Four corn hybrid seed lots produced from reciprocal crosses were compared (HS 15 with HS 51, and HS 24 with HS 42) by assessing germination, vigour, and seedling emergence in the field. Seed composition was assessed in mature, dehydrated seeds. Proteins were extracted, quantified, and analysed by electrophoresis and densitometry. Starch amounts were assessed using a kit and soluble sugars were determined using high performance liquid chromatography with pulsed electrochemical detection. The L1 and L2 lineages, used as female parents, provided seeds with lower vigour; however, the quantification of major protein bands, and sucrose, raffinose and stachyose were similar between seed lot pairs. While both total seed protein and starch varied between reciprocal hybrids for one of the two sets of crosses, the amounts of neither correlated with seed vigour. Interestingly, hybrids with low seed vigour (HS 15, HS 24) accumulated greater amounts of fructose relative to their reciprocal; correlation analysis confirmed these results. We demonstrate different effects on seed vigour dependent on the maternal parent in reciprocal crosses producing hybrid corn seeds. We also show that vigour is negatively correlated with seed reducing sugar contents.

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“…In soybean four QTL for oligosaccharide content were identified (Kim et al 2006). In Medicago truncatula QTL mapping revealed one QTL that explained variation in the stachyose/verbascose ratio co-located with a stachyose synthase gene, determining the oligosaccharide composition (Vandecasteele et al 2011). For QTL analyses saturated genetic maps are necessary.…”

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confidence: 99%

Variability in the oligosaccharide concentration in seeds of the mapping population of pea (Pisum sativum L.)

Gawłowska

Lahuta²,

Świecicki

et al. 2014

Czech J. Genet. Plant Breed.

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Gawłowska M., Lahuta L., Święcicki W., Krajewski P. (2014): Variability in the oligosaccharide concentration in seeds of the mapping population of pea (Pisum sativum L.). Czech J. Genet. Plant Breed., 50: 157-162.Anti-nutritional compounds are among the obstacles to the use of pea seeds as a protein source in both feed and food. These compounds are poorly digested by both monogastric animals and humans. There are three main oligosaccharides in pea: raffinose, stachyose and verbascose (raffinose family oligosaccharides -RFOs). The concentration of oligosaccharides in dry seeds, the oligosaccharide percent to the total content of soluble sugars and quantitative trait loci (QTLs) were analysed in the mapping population Wt10245 × Wt11238. The composition and concentration of soluble carbohydrates in seeds harvested from two field experiments (2002 and 2004) were analysed by the high resolution gas chromatography method. The Wt10245 × Wt11238 population was chosen because of the greater difference in the concentration of RFOs in seeds between parental lines (56.48 mg/g seed in Wt10245 and 99.1 mg/g seed in Wt11238). The average levels of oligosaccharides (mg/g seed) from both field experiments in the mapping population were: myo-inositol 1.5, sucrose 33.3, galactinol 0.8, raffinose 9.6, stachyose 30.1, verbascose 37.1. The total oligosaccharide concentration was 76.8 mg/g seed. This comprised anaverage of 68% soluble sugars, with the range from 59% to 75%. The results are in agreement with the knowledge of RFO biosynthesis. This makes selection for changes in the proportion of the particular oligosaccharides difficult, like in Phaseolus. However, it is possible to decrease the RFO content in pea seeds. The linkage between QTL and the gene r is interesting. The rugosus (r) locus changes the morphology and distribution of starch grains, decreases the total starch accumulation, produces a higher ratio of amylose to amylopectin and higher sugar and water content during development along with changes in cell size and lipid content.Keywords: genetic map; molecular markers; QTL; rugosus (r) locus; seed oligosaccharide Pea seeds are an important source of protein. However, their value as ingredients in food and feed is limited by a considerable amount of anti-nutritional factors, among them sucrose α-galactosides (raffinose, stachyose and verbascose, named the raffinose family of oligosaccharides, RFOs). Because of the lack of α-galactosidase in the human digestive system, RFOs appear unchanged in the large intestine, where they are degraded and fermented by microflora, causing cramps and flatulence (Coon et al. 1990). In effect, the true metabolizable energy and digestibility can be decreased. However, the presence of RFOs in the digestive tract stimulates development of some probiotic bacteria. The nutritional importance of

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Quantitative trait loci analysis reveals a correlation between the ratio of sucrose/raffinose family oligosaccharides and seed vigour in Medicago truncatula

Cited by 74 publications

References 37 publications

Evidence for participation of the methionine sulfoxide reductase repair system in plant seed longevity

Evidence for participation of the methionine sulfoxide reductase repair system in plant seed longevity

Reciprocal effect of parental lines on the physiological potential and seed composition of corn hybrid seeds

Variability in the oligosaccharide concentration in seeds of the mapping population of pea (Pisum sativum L.)

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