Quantitative trait loci analysis of melon (Cucumis melo L.) domestication-related traits

Díaz, Aurora; Martín-Hernández, Ana Montserrat; Dolcet-Sanjuan, Ramón; Garcés-Claver, Ana; Álvarez, J. M.; García-Más, Jordi; Picó, Belén; Monforte, Antonio J.

doi:10.1007/s00122-017-2928-y

Cited by 41 publications

(50 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This additional crop wild relative has been overlooked for decades mainly because of taxonomic confusion. Material originally distributed by North Central Regional Plant Introduction Station (Ames, IA, USA) of the USDA-ARS, as "Cucumis trigonus Ames 24297" and widely used in genomic studies, including some of our own work (Sebastian et al, 2010;Díaz et al, 2017), does not belong to C. trigonus Roxb. but instead represents the common Asian wild melon form C. melo subsp.…”

Section: Two Closest Wild Relatives Of the Melon Clade One From Austmentioning

confidence: 99%

Repeated domestication of melon (Cucumis melo) in Africa and Asia and a new close relative from India

Endl

Achigan‐Dako

Pandey

et al. 2018

American J of Botany

View full text Add to dashboard Cite

Premise of the StudyThe domestication history of melon is still unclear. An African or Asian origin has been suggested, but its closest wild relative was recently revealed to be an Australian species. The complicated taxonomic history of melon has resulted in additional confusion, with a high number of misidentified germplasm collections currently used by breeders and in genomics research.MethodsUsing seven DNA regions sequenced for 90% of the genus and the major cultivar groups, we sort out described names and infer evolutionary origins and domestication centers.Key ResultsWe found that modern melon cultivars go back to two lineages, which diverged ca. 2 million years ago. One is restricted to Asia (Cucumis melo subsp. melo), and the second, here described as C. melo subsp. meloides, is restricted to Africa. The Asian lineage has given rise to the widely commercialized cultivar groups and their market types, while the African lineage gave rise to cultivars still grown in the Sudanian region. We show that C. trigonus, an overlooked perennial and drought‐tolerant species from India is among the closest living relatives of C. melo.ConclusionsMelon was domesticated at least twice: in Africa and Asia. The African lineage and the Indian C. trigonus are exciting new resources for breeding of melons tolerant to climate change.

show abstract

Section: Two Closest Wild Relatives Of the Melon Clade One From Austmentioning

confidence: 99%

Repeated domestication of melon (Cucumis melo) in Africa and Asia and a new close relative from India

Endl

Achigan‐Dako

Pandey

et al. 2018

American J of Botany

View full text Add to dashboard Cite

show abstract

“…Construction of the PS × TRI mapping populations are described in detail elsewhere (Diaz et al, 2017 ). Briefly, a F 2 population of 200 plants was acclimated and transplanted for three independent experiments in the same facilities described above at COMAV-UPV in 2011 (VAL11) and Zaragoza (Centro de Investigación y Tecnología Agroalimentaria de Aragón, CITA, 41° 43′26″ N—0° 48′ 31″ O) in 2011 and 2012 (Z11 and Z12).…”

Section: Methodsmentioning

confidence: 99%

“…In order to identify QTL with stable effects and gain more insight on the genetic factors involved in sugar accumulation in melon fruits, we studied sugar content in the full set of the PS × SC NILs in three environments, and in several different populations derived from the cross between the same PS cultivar and the wild melon accession “Trigonus” (TRI) accession Ames 24294 ( C. melo ssp. agrestis group agrestis ) that was developed recently (Diaz et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

QTL Analyses in Multiple Populations Employed for the Fine Mapping and Identification of Candidate Genes at a Locus Affecting Sugar Accumulation in Melon (Cucumis melo L.)

et al. 2017

Self Cite

View full text Add to dashboard Cite

Sugar content is the major determinant of both fruit quality and consumer acceptance in melon (Cucumis melo L), and is a primary target for crop improvement. Near-isogenic lines (NILs) derived from the intraspecific cross between a “Piel de Sapo” (PS) type and the exotic cultivar “Songwhan Charmi” (SC), and several populations generated from the cross of PS × Ames 24294 (“Trigonus”), a wild melon, were used to identify QTL related to sugar and organic acid composition. Seventy-eight QTL were detected across several locations and different years, with three important clusters related to sugar content located on chromosomes 4, 5, and 7. Two PS × SC NILs (SC5-1 and SC5-2) sharing a common genomic interval of 1.7 Mb at the top of chromosome 5 contained QTL reducing soluble solids content (SSC) and sucrose content by an average of 29 and 68%, respectively. This cluster collocated with QTL affecting sugar content identified in other studies in lines developed from the PS × SC cross and supported the presence of a stable consensus locus involved in sugar accumulation that we named SUCQSC5.1. QTL reducing soluble solids and sucrose content identified in the “Trigonus” mapping populations, as well as QTL identified in previous studies from other ssp. agrestis sources, collocated with SUCQSC5.1, suggesting that they may be allelic and implying a role in domestication. In subNILs derived from the PS × SC5-1 cross, SUCQSC5.1 reduced SSC and sucrose content by an average of 18 and 34%, respectively, and was fine-mapped to a 56.1 kb interval containing four genes. Expression analysis of the candidate genes in mature fruit showed differences between the subNILs with PS alleles that were “high” sugar and SC alleles of “low” sugar phenotypes for MELO3C014519, encoding a putative BEL1-like homeodomain protein. Sequence differences in the gene predicted to affect protein function were restricted to SC and other ssp. agrestis cultivar groups. These results provide the basis for further investigation of genes affecting sugar accumulation in melon.

show abstract

“…DNA extraction and microsatellite genotyping DNA from young leaves was extracted according to protocols previously described (Doyle and Doyle 1990), with slight modifications (Díaz et al 2017).…”

Section: Plant Materialsmentioning

confidence: 99%

The emergence of a new weed in maize plantations: characterization and genetic structure using microsatellite markers

Díaz

Taberner

Vilaplana

2019

Genet Resour Crop Evol

View full text Add to dashboard Cite

The objective of this work was to assess the genetic variability and structure of a new weed in Spanish maize fields, and investigate its geographical patterns using 17 microsatellites. Commercial maize varieties (C), maize-like weeds (MLW), putative hybrids with C (WCH), and teosintes (Tm: Zea mays ssp. mexicana and Tp: Z. mays ssp. parviglumis) were analyzed. The weed genetic diversity (MLW and WCH: 0.52) was the lowest (C: 0.59, Tm: 0.66, and Tp: 0.71). Weeds (0.21) and teosintes (Tm: 0.27, Tp: 0.34) showed positive values for the inbreeding coefficient (FIS), which agrees with their low values for the observed heterozygosity (HO), common in wild species; whereas C exhibited a negative FIS value (− 0.06, excess of heterozygous), common in domesticated species. Major clustering agreed with the different types of samples, even if some of the most hybridized weeds branched with the C cluster. Within the weeds, an evident tendency to group together depending on their geographical origin was perceived. Structure analyses confirmed the contribution of C to the genome of those weeds with the highest degree of hybridization. Consistently, the genetic variation (FST) was not negligible only when the teosintes were compared to the C group. Most of the molecular variance occurred within populations (51.83%) and not among populations (10.09%), with the highest value (32.33%) being found within the weed population. These new weeds seem to have a complex origin. Even if they are related to both, C and teosintes (Tm and Tp), they form an unidentified and genetically distinct group (FST: 0.13).

show abstract

Quantitative trait loci analysis of melon (Cucumis melo L.) domestication-related traits

Cited by 41 publications

References 53 publications

Repeated domestication of melon (Cucumis melo) in Africa and Asia and a new close relative from India

Repeated domestication of melon (Cucumis melo) in Africa and Asia and a new close relative from India

QTL Analyses in Multiple Populations Employed for the Fine Mapping and Identification of Candidate Genes at a Locus Affecting Sugar Accumulation in Melon (Cucumis melo L.)

The emergence of a new weed in maize plantations: characterization and genetic structure using microsatellite markers

Contact Info

Product

Resources

About