Quantitative trait loci analysis of mineral element concentrations in an Arabidopsis halleri × Arabidopsis lyrata petraea F₂ progeny grown on cadmium‐contaminated soil

Abstract

Summary• This study describes the quantitative trait locus (QTL) analysis of cadmium (Cd), zinc (Zn), iron (Fe), potassium (K), magnesium (Mg) and calcium (Ca) accumulation in the pseudometallophyte Arabidopsis halleri under conditions of Cd excess using an interspecific A. halleri · Arabidopsis lyrata F 2 population.• Our data provide evidence for the implication of one major QTL in Cd hyperaccumulation in A. halleri, and suggests that Cd tolerance and accumulation are not independent in A. halleri. Moreover, the major loci responsible for Zn hyperaccumulation in the absence of Cd appear to be the same when Cd is present at high concentrations.• More than twofold higher Fe concentrations were measured in A. halleri shoots than in A. lyrata, suggesting a different regulation of Fe accumulation in the hyperaccumulator.• With the exception of Ca, the accumulation of Cd was significantly correlated with the accumulation of all elements measured in the F 2 progeny, suggesting pleiotropic gene action. However, QTL analysis identified pleiotropic QTLs only for Cd, Zn and Fe. Mg accumulation was negatively correlated with Cd accumulation, as well as with dry shoot biomass, suggesting that it might indicate cellular damage.

Mapping of Iron and Zinc Quantitative Trait Loci in Soybean for Association to Iron Deficiency Chlorosis Resistance

“…Several reports are available on mapping QTL for seed or grain Zn and Fe concentrations and contents in various species (Zhou et al, 2010;Garcia-Oliveira et al, 2009;Ding et al, 2010;Tiwari et al, 2009). These reports identified as few as one significant QTL for Fe and Zn in wheat grain, and as many as five Zn and four Fe QTL in Arabidopsis halleri (Tiwari et al, 2009;Willems et al, 2010). We report here the first QTL for soybean seed Fe and Zn concentration and their relationship with Fe efficiency.…”

“…For seed Fe concentration, two suggestive QTL were detected in 2008 (Table 5), whereas, one significant QTL and one suggestive QTL were detected in 2009. Suggestive QTL were those QTL that had peaks that did not exceed the genome-wide threshold but on the chromosome level were close to or were significant at P = 0.05 (Tiwari et al, 2009;Willems et al, 2010). Those four QTL were each on different chromosomes, with the significant QTL on chromosome 1 in the marker interval Satt295-Satt383.…”

Evaluation and QTL mapping of phosphorus concentration in soybean seed

“…In 2008, three suggestive QTL were detected on chromosomes 10, 18, and 20 (Table 3). Suggestive QTL were those QTL possessing peaks in LOD scores that did not exceed the genome wide threshold, but on the chromosome level were at or exceeded significant P \ 0.05 (Liu et al 2007;Tiwari et al 2009;Willems et al 2010). The LOD scores of these QTL ranged from 2.6 to 2.9 and the variation explained was 11.9-19.4 %.…”

How metal-tolerant ecotypes of ectomycorrhizal fungi protect plants from heavy metal pollution

“…In bacteria, plasmids play a major role in this multiple heavy metal tolerance. In most eukaryotic organisms, true adaptation seems to be governed by a relatively small number of genetic determinants Willems et al 2010). Nevertheless, such genetic modifications occur slowly, in particular in species with long reproductive cycles.…”