Quantitative trait analysis of resistance to plum pox virus in the apricot F1 progeny “Harlayne” × “Vestar”

Abstract

Plum pox virus (PPV) is a devastating stone fruit disease of major importance, and better understanding of the genetic control of resistance to this trait would be useful for more efficient development of resistant cultivars. Previous studies have reported a locus of major effect from PPV resistance on linkage group 1. The current study confirms these results by mapping plum pox virus resistance in a F1 progeny issued from a cross between "Harlayne", as a PPV-resistant parent, and "Vestar" as a susceptible parent. The hybrids were grafted simultaneously and subsequently inoculated with the PPV-M and D strains.The symptom scoring on leaves was performed nine times over two vegetative cycles. Marker-trait associations were analyzed using the Kruskal-Wallis (KW) non-parametric test, and the PPV resistance loci were mapped using composite interval mapping (CIM). We show that both analyses (KW and CIM) highlighted the upper part of linkage group 1 of the apricot "Harlayne" genitor.

Genomic analysis reveals MATH gene(s) as candidate(s) for Plum pox virus (PPV) resistance in apricot (Prunus armeniaca L.)

“…The genetic control of PPV resistance in apricot has remained controversial; however, most inheritance studies agree on the importance of the involvement of one major dominant locus (the PPVres locus) located in the upper part of apricot linkage group 1 (Dondini et al ., ; Lalli et al ., ; Lambert et al ., ; Marandel et al ., ; Pilarova et al ., ; Soriano et al ., ). More recently, Vera‐Ruiz et al .…”

Evaluation of Turkish apricot germplasm using SSR markers: Genetic diversity assessment and search for Plum pox virus resistance alleles

“…Due to the difficulties associated with woody plant breeding, the development of resistance-related molecular markers to be used in marker-assisted selection (MAS) has become of vital importance. Genetics maps of apricots have been developed for PPV resistance (Hurtado et al, 2002;Lambert et al, 2007;Soriano et al, 2008;Lalli et al, 2008;Marandel et al, 2009;Pilarová et al, 2010;Dondini et al, 2011;Vera Ruiz et al, 2011) and resistance-related molecular markers were searched. A http://dx.doi.org/10.1016/j.scienta.2015.07.012 0304-4238/© 2015 Elsevier B.V. All rights reserved.…”

Narrowing down the apricot Plum pox virus resistance locus and comparative analysis with the peach genome syntenic region

“…Together, BTL mapping and graphical genotyping suggest that PPVres is located within the interval defined by Gol027 and Gol061 markers. This position is roughly consistent with those previously suggested in other P. armeniaca genetic backgrounds (Marandel et al ., 2009a; Pilarova et al ., 2010) and in P. davidiana (Marandel et al ., 2009b; Rubio et al ., 2010), and particularly coincident with those proposed by Lambert et al . (2007) and Dondini et al .…”

“…Recent studies on the segregation of different intraspecific apricot crosses have shown that PPV resistance is controlled by at least one major dominant locus (hereafter termed PPVres ) located on the upper part of apricot linkage group 1 (LG1) (Dondini et al ., 2010; Lalli et al ., 2008; Lambert et al ., 2007; Marandel et al ., 2009a; Pilarova et al ., 2010; Soriano et al ., 2008). Nevertheless, mapping was not accurate in any case and map positions predicted for PPVres varied from one study to another.…”

“…Nevertheless, mapping was not accurate in any case and map positions predicted for PPVres varied from one study to another. Moreover, other minor loci have been suggested to underlie PPV resistance in apricot (Lambert et al ., 2007; Pilarova et al ., 2010). The scenario becomes more complex when PPV resistance derived from the Prunus davidiana P1908 clone is analysed.…”