Quantitative Thin-Layer Chromatography

Thin-layer chromatography of green leaves extracts: Zinc migrates into pheophytin on TLC silica plates with fluorescence indicator (F254)

“…For example, Quach et al state that comparable results were obtained on TLC plates with and without fluorescent indicator [13]. However, in their Figure 1B, both pheophytin a and b are thoroughly green.…”

“…This aspect was, to our knowledge, never discussed in textbooks or general chromatography books, e.g., [4][5][6], or in the specific literature on thin-layer chromatography [7][8][9][10][11][12][13][14]. Problems with the fluorescent indicator, F 254 , added to TLC plates have, however, been reported [15].…”

Quantitative High Performance Thin Layer Chromatography for the Analysis of Herbal Medicines: Problems and Advantages

“…High Performance Thin Layer Chromatography (HPTLC) plates provide has higher surface area and allows for quicker and clearer sample separation due to more consistent and significantly smaller particle size of the silica [12]. The use of HPTLC plates in combination with automated sample applicators and development chambers, high resolution cameras, and computing software allows more control over experimental conditions and greater data analysis capabilities [11,13].…”

Quantitative two-dimensional thin-layer chromatography using a diode-array detector

“…The same area of interest measured at different wavelengths in absorption (1)(2)(3)(4)(5) and fluorescence (6) shows that this method can improve the spot resolution Aalen, Germany) was used with an reflection attachment of 9 identical optical fibers ( Figure 3.3-1) with a diameter of 100 µm each (produced by TransMITCentre for Fibre Optics and Industrial Laser Applications, Gießen, Germany).…”

“…Thin layer chromatography as a space separation method is by far the simplest separation technique considered in terms of equipment and performance [1]. Multidimensional chromatography is the most powerful tool for separations [2].…”