Quantitative telomerase activity in circulating human leukocytes: utility of real-time telomeric repeats amplification protocol (RQ-TRAP) in a clinical/epidemiological setting

Lança, Vasco; Zee, Robert Y.L.; Rivera, Alicia; Romero, José R.

doi:10.1515/cclm.2009.188

Cited by 3 publications

(3 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A highly significant difference in telomerase activity was detected between the threshold cycle (Ct) measurements (Ct ± SE) of SAMP8 mice treated with GHRH antagonist MZ-5-156 and controls. Values for Ct are inversely related to telomerase gene activity, so that a decrease in Ct corresponds to an increase in telomerase activity (7,15,16). The hearts of MZ-5-156-treated mice showed a statistically significant decrease in Ct activity (MZ-5-156, 21.1 ± 0.7; control, 23.3 ± 0.2, P < 0.005), corresponding to a 4.7-fold increase in telomerase activity.…”

Section: Resultsmentioning

confidence: 99%

“…Quantitative real-time PCR based on the SYBR green high-sensitivity telomeric repeat amplification protocol (TRAP) was used for rapidly quantifying telomerase activity. Decreases in Ct values correspond to an increase in telomerase activity (15,16). Results are presented as Ct to allow for comparability with other studies on telomerase activity (15,16).…”

Section: Controlmentioning

confidence: 99%

“…Decreases in Ct values correspond to an increase in telomerase activity (15,16). Results are presented as Ct to allow for comparability with other studies on telomerase activity (15,16). Ct values are determined from semilog amplification plots (log increase in fluorescence vs. cycle number) compared with standard curves generated from serial dilutions of telomerase TSR8 control template (30).…”

Section: Controlmentioning

confidence: 99%

See 2 more Smart Citations

Effects of a growth hormone-releasing hormone antagonist on telomerase activity, oxidative stress, longevity, and aging in mice

Banks

Morley

Farr

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Both deficiency and excess of growth hormone (GH) are associated with increased mortality and morbidity. GH replacement in otherwise healthy subjects leads to complications, whereas individuals with isolated GH deficiency such as Laron dwarfs show increased life span. Here, we determined the effects of treatment with the GH-releasing hormone (GHRH) receptor antagonist MZ-5-156 on aging in SAMP8 mice, a strain that develops with aging cognitive deficits and has a shortened life expectancy. Starting at age 10 mo, mice received daily s.c. injections of 10 μg/mouse of MZ-5-156. Mice treated for 4 mo with MZ-5-156 showed increased telomerase activity, improvement in some measures of oxidative stress in brain, and improved pole balance, but no change in muscle strength. MZ-5-156 improved cognition after 2 mo and 4 mo, but not after 7 mo of treatment (ages 12, 14 mo, and 17 mo, respectively). Mean life expectancy increased by 8 wk with no increase in maximal life span, and tumor incidence decreased from 10 to 1.7%. These results show that treatment with a GHRH antagonist has positive effects on some aspects of aging, including an increase in telomerase activity.Alzheimer's disease | learning | memory | peptide | sarcopenia T he aging process is associated with decreased physical and mental functioning, increased morbidity, and inevitable mortality. Specific neurodegenerative diseases such as Alzheimer's disease lead to decreased learning and memory. The incidence of many benign and malignant tumors clearly increases with aging. Populations show significant variations in the rate at which aging advances and many mechanisms have been proposed to explain aging or were found to be associated with the rate of aging. Oxidative stress, telomerase activity, and variation in hormonal levels are examples of these associations and mechanisms.Among hormones that are implicated in aging is growth hormone (GH). GH levels decrease dramatically with aging (1) and some of the findings in aging, such as thinning of the skin and bones, are the opposite of those found in acromegaly, a condition in which GH is overproduced (2). This has led to the proposal that replacement of GH will reverse or slow the aging process. However, several clinical trials showed that replacement of GH is associated with increased mortality (3, 4). Acromegaly is also linked to many adverse conditions that are associated with aging, including glucose intolerance, heart disease, and an increased cancer incidence (5). Transgenic mice overexpressing GH have a decreased life span and evidence of accelerated aging in several organs and tissues, including the brain (6).Whereas GH replacement and acromegaly are associated with adverse outcomes, GH deficiency or resistance to GH are associated with longer life expectancy. Laron dwarfs show resistance to GH at the receptor level and so do not secrete insulin-like growth factor I (IGF-I) and can live to an advanced age (2). Mouse strains with a deficiency in GH activity because of transcription factor mutations (the Ames m...

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Controlmentioning

confidence: 99%

Section: Controlmentioning

confidence: 99%

See 1 more Smart Citation

Effects of a growth hormone-releasing hormone antagonist on telomerase activity, oxidative stress, longevity, and aging in mice

Banks

Morley

Farr

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

Signal-On Dual-Potential Electrochemiluminescence Based on Luminol–Gold Bifunctional Nanoparticles for Telomerase Detection

Zhang

et al. 2014

Anal. Chem.

185

View full text Add to dashboard Cite

Here, we report a novel type of signal-on dual-potential electrochemiluminescence (ECL) approach for telomerase detection based on bifunctionalized luminol-gold nanoparticles (L-Au NPs). In this approach, CdS nanocrystals (NCs) were first coated on glassy carbon electrode, and then thiol-modified telomerase primer was attached on CdS NCs via Cd-S bond. In the presence of telomerase and dNTPs, the primer could be extended. Telomerase primer would hybridize with its complementary DNA, and the extended part would hybridize with the capture DNA which was tagged with L-Au NPs. In the presence of coreactant H2O2, the L-Au NPs could not only enhance the ECL intensity of CdS NCs at -1.25 V (vs SCE) induced by the surface plasmon resonance (SPR) of Au NPs but also produce a new ECL signal at +0.45 V (vs SCE) that resulted from luminol in L-Au NPs. Both signals at two potentials increased with the increase of telomerase concentration. This method could be used to detect the telomerase from 100 to 9000 HL-60 cells and investigate the apoptosis of tumor cells. The ratio of the two signal increments (ΔECL(Luminol)/ΔECL(CdS NCs)), which showed a high consistency value for different numbers of cells, could be used to verify the reliability of tests. This dual-potential ECL strategy showed great promise in avoiding false positive or negative results in bioanalysis.

show abstract

Telomerase stability and evaluation of real-time telomeric repeat amplification protocol

Vukašinović

Kotur‐Stevuljević

Mlakar

et al. 2019

Scandinavian Journal of Clinical and Laboratory Investigation

View full text Add to dashboard Cite

Quantitative telomerase activity in circulating human leukocytes: utility of real-time telomeric repeats amplification protocol (RQ-TRAP) in a clinical/epidemiological setting

Cited by 3 publications

References 17 publications

Effects of a growth hormone-releasing hormone antagonist on telomerase activity, oxidative stress, longevity, and aging in mice

Effects of a growth hormone-releasing hormone antagonist on telomerase activity, oxidative stress, longevity, and aging in mice

Signal-On Dual-Potential Electrochemiluminescence Based on Luminol–Gold Bifunctional Nanoparticles for Telomerase Detection

Telomerase stability and evaluation of real-time telomeric repeat amplification protocol

Contact Info

Product

Resources

About