Quantitative¹HNMR spectroscopy for the determination of oxyresveratrol inArtocarpus lacuchaheartwood

Abstract

Introduction Quantitative nuclear magnetic resonance (qNMR) spectroscopy is an analytical method based on the principles of NMR spectroscopy. The main advantages of this method are its simplicity, time efficiency, high accuracy and reproducibility, and it is a non‐destructive technique. Objective To evaluate and standardise the quality of Artocarpus lacucha heartwood. A method for quantifying its oxyresveratrol content using qNMR was developed. Methodology Proton (1H)NMR (400 MHz) spectroscopy was used to analyse the methanol‐d4 solution of a given amount of crude extract of A. lacucha heartwood using ethyl p‐methoxycinnamate (EPMC) as an internal standard. The qNMR methodology was validated in terms of its linearity and range, limit of quantification (LOQ), stability, precision, and accuracy for the determination of the oxyresveratrol content. Results The qNMR method was validated in terms of its linearity, range, LOQ, accuracy, precision, and stability. The quantitative determination of the oxyresveratrol content in the methanolic crude extract of A. lacucha was found to be 17% based on 1HNMR analysis, which proved to be a reliable method as the results were comparable to those obtained by high‐performance liquid chromatography (HPLC) analysis. Conclusions This study validated qNMR spectroscopy as a reliable analytical procedure to determine oxyresveratrol in A. lacucha heartwood. Therefore, this qNMR method can serve as an alternative to the classical HPLC methods for evaluating and standardising the quality of A. lacucha heartwood.

Oxyresveratrol: Sources, Productions, Biological Activities, Pharmacokinetics, and Delivery Systems

“…The physical constants and spectroscopic properties (particularly NMR data) of both natural and synthetic ORV are presented in numerous reports [ 14 , 21 , 34 , 42 , 49 , 56 , 57 , 72 , 76 , 107 , 128 ]. The data for the crystal structure of ORV in the form of dihydrate are also available [ 129 ].…”

Rapid and reliable distinguish of Morinda officinalis How by loop-mediated isothermal amplification (LAMP) assay

“…Sinensis). At present, some studies have reported various of novel methods of medicinal plants identi cation, such as barcode-based melting curve analysis, DNA labeling or 1 HNMR metabolite ngerprinting and so on 26,27 . Although these methods can accurately identify species, they require specialized knowledge, statistical analysis, and complex instruments.…”

Quantitative Determination of Acrolein in Cider by 1H NMR Spectrometry

“…The nuclear magnetic resonance (NMR) technique can detect a large number of components simultaneously in complex mixtures. In addition, sample preparation for NMR spectroscopy is simpler and less time consuming than for chromatography [18][19][20]. The purpose of this work is to provide a simple and fast procedure to determine acrolein in cider by proton nuclear magnetic resonance ( 1 H NMR), in the same way that our research group has already achieved with other analytes, for example, caffeine, trigonelline, formic acid, and 5-(hydroxymethyl)furfural in soluble coffees [21].…”