Quantitative Study on Human Cerebellar Cortex from Anatomy Cadaver Preparations

Kalanjati, Viskasari Pintoko; Dewi, Arni Kusuma; Santoso, M. W. A

doi:10.4067/s0717-95022017000100027

Cited by 5 publications

(8 citation statements)

References 7 publications

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“…In humans, the cellular cortical lamination is arranged in both horizontal and vertical (or radial, columnar) networks [9][10][11]. Using magnetic resonance microscopy at 9.4 T, a highly specific horizontal pattern of lamination in the human cortical brain, including the neocortex, was shown [9][10][11][12][13]. A specific pattern of cortical lamination has been reported to correspond to unique predominant functions in each area.…”

Section: Discussionmentioning

confidence: 99%

“…All bodies were subjected immediately after death to injection of a formalin (10%) fixative solution via the femoral artery six months prior to the current study. As was shown, the respective samples of brain tissues are characterized by relatively good quality for a further histological study [7][8][9]. After removing the braincase, the whole encephalon was removed, processed for histology slides (4 µm-thick parasagittal slices), and stained with hematoxyllin-eosin using standard routine techniques.…”

Section: Methodsmentioning

confidence: 99%

“…The nuclei of the microgliocytes and astrocytes were differentiated by their small dimensions, an openfaced, oval, or rounded form, or by the slightly larger nuclei with some processes, respectively [7][8][9].…”

Section: Methodsmentioning

confidence: 99%

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Cellular Composition in the Aging Cerebral Cortex of Humans

2019

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The numbers/densities of neurons and gliocytes and the neuron-to-glia ratios were calculated in different layers of the cerebral cortex of three subjects in a post mortem study. The subjects were 60 to 70 years old (which was qualified as a relatively early period of aging) and had no history of neurodegeneration diseases. The brains embalmed in 10% formalin-fixative solution were processed, and coronal 4-µmthick slices of tissues of the frontal, parietal, temporal, and occipital lobes were prepared and stained with hematoxylin-eosin. Clear cortical lamination was observed in all lobes. In the frontal lobe, the mean neuron density (number of neurons within visual fields at a 400× magnification) was significantly higher, while that of gliocytes was significantly lower compared to the respective values in other lobes (P < 0.05). A significant association between the number of neurons and glia was found in the frontal lobe (r 2 = 0.40, P = 0.0029) and in all superficial cortical layers (r 2 = 0.72, P = 0.0025), but not in the deeper layers (r 2 = 0.18, P = 0.14). The mean neuron-to-glia ratio in different lobes and layers varied from 1.0 : 0.81 to 1.0 : 3.54. It is concluded that, in early-aging subjects with no cerebral pathology, the quantitative characteristics of cortical neurons and glial cells do not differ significantly from those in the brains of younger adult healthy subjects.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Cellular Composition in the Aging Cerebral Cortex of Humans

2019

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“…Cerebellum is an important part of the brain. It acts as a coordinator of impulses comes to and from the cerebral cortex [7]. Hence, cerebellar abnormalities may lead to impairment of cognitive functions [8] motor coordination, smooth movement [9], and vision ability [10].…”

Section: Introductionmentioning

confidence: 99%

Comparative morphological, histological, and RAPD analysis of Columba livia domestica strains

Sabry¹,

Yosra²

2020

J App Biol Biotech.

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The present study focused on the variations of morphological and cerebellar histological structures of five strains of adult male Columba livia domestica (n = 5) and their genetic polymorphism using RAPD-PCR technique to differentiate between them and find the best strains that can adapt to different circumstances. In each strain, the beak length and the eye diameter were measured in relation to the head length. Specimens of the cerebellum were fixed and processed for histological investigations. The thickness of the cerebellar cortex layers and the numbers of Purkinje cells was determined. For genetic polymorphism, 20 RAPD primers were used to determine the genetic diversity between the studied strains. Balady strain could express the best adaptation if subjected to feed restrictions; however, its low Purkinje cells number may reveal its low cognitive ability compared to the other studied strains. Primers OPA-1, OPA-3, and OPA-6 are the best used primers to differentiate between the studied strains. The highest similarity coefficient was detected between Halaby and Gamey strains, while, Balady strain was completely separated and independent. In conclusion, in addition to the morphological and histological variations between studied strains, the RAPD markers are recommended as a valuable tool to verify the genetic polymorphism of Columba livia domestica strains.

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“…SABRY creates motor-related outputs which depend on the cerebellar neuronal networks (Apps & Garwicz, 2005). It also functions as an impulse coordinator that comes in and out of the cerebral cortex (Kalanjati, Dewi, & Santoso, 2017). Therefore, cerebellar defects may result in impairment of cognitive functions (Glickstein, Strata, & Voogd, 2009), motor coordination, smooth motion (Bastian, 2006) and vision ability (Crossman & Neary 2015).…”

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confidence: 99%

Comparative study about ageing effect on retina and cerebellum of Columba livia domestica

Sabry

2020

Acta Zoologica

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The present research explores the ageing impact of Columba livia domestica on retina and cerebellum. It is an available and a widespread bird species. Three ageing groups (n = 5) of male Columba were collected at about three weeks, six months and five years old. Histological studies were made. Morphometric measurement of both retinal and cerebellar cortex layer thicknesses has been determined. For evaluation of the neuronal synapses, quantitative GFAP biochemically and immunohistochemically staining was performed. Also, biochemical analysis of glucose transporter 1, serotonin and dopamine was carried out to demonstrate their functional activity.Flow cytometric assessments of quantitative cell death were accomplished by using annexin V. The present findings revealed quantitative and qualitative increase of GFAP parallel with decrease of serotonin, dopamine and glucose transporter 1 as well as increase of UR and LR annexin V reflecting cell death in old groups. These were confirmed by damaging of the retina and cerebellar layers. The present findings concluded that retinal and cerebellar cells associated with ageing were determined by sharp rise in both cell death and GFAP levels. Additionally, a decrease of dopamine, serotonin and GluT1 level manifested maybe led to functional alteration of both retina and brain. K E Y W O R D Sageing, annexin V, cerebellum, Columba livia domestica, histology, retina | 395 SABRY triggered during flight by acquiring information about the optic flow (Wylie et al., 2018). In pigeons, the visual motion takes place throughout the retina via self-motion as self-rotation and self-translation is affected by information inputs in vestibulocerebellum (Wylie, 2013). Both retina and cerebellum also play an essential role for locomotion of pigeons for food collection and avoiding predators (Dhawan, 1991). Consequently, both retina and cerebellum are of great importance in managing the pigeon activity.Birds showed higher metabolic rate (2-2.5 times) (Butler, 2016) and two-to-three times longer life spans compared to mammals of the same size (Travin & Feniouk, 2016). These may result from certain adaptation for inhibiting ageing-related damage induced by reactive oxygen species and formation of glycation end products (Holmes, Fluckiger, & Austad, 2001). Also, birds characterized by their higher body temperature (3-5°C) and blood glucose (2-4 times) than mammals (Ritchie, Harrison, & Harrison, 1994) of similar body mass. Mammalian and avian species of more than one kilogram showed blood glucose concentration within the range of about 135 and 275 mg/dl, respectively (Braun & Sweazea, 2008). These manifestations supposed to accelerate ageing in birds due to the production of glycosylated proteins via Maillard reaction (Monnier, 1990;Kristal & Yu, 1992). However, they show increased longevity (Skujina, McMahon, Lenis, Gkoutos, & Hegarty, 2016). This arouses curiosity about the comparable changes of ageing among avian and mammalian species (Holmes et al., 2001) and attracts many authors such as Holme...

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Quantitative Study on Human Cerebellar Cortex from Anatomy Cadaver Preparations

Cited by 5 publications

References 7 publications

Cellular Composition in the Aging Cerebral Cortex of Humans

Cellular Composition in the Aging Cerebral Cortex of Humans

Comparative morphological, histological, and RAPD analysis of Columba livia domestica strains

Comparative study about ageing effect on retina and cerebellum of Columba livia domestica

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