Quantitative study of zinc and metallothioneins in the human retina and RPE cells by mass spectrometry-based methodologies

Rodríguez-Menéndez, Sara; Fernández, Beatriz; García, Montserrat; Álvarez, Lydia; Fernández, María Luisa López; Sanz‐Medel, Alfredo; Coca‐Prados, Miguel; Pereiro, Rosario; González‐Iglesias, Héctor

doi:10.1016/j.talanta.2017.09.024

Cited by 22 publications

(33 citation statements)

References 32 publications

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“…The increased levels of zinc found in the sub‐RPE space could be explained by the dysfunction of zinc‐rich RPE, occurring in disease states. Indeed, elemental distribution of Zn showed preferential decrease of zinc in the RPE layer in disease . Zinc content and retention is mediated, at least partly, by pigmentation of the RPE, as melanin synthesis and melanosome formation appears to be regulated by zinc .…”

Section: Zinc In the Normal Retinamentioning

confidence: 98%

“…The retina–choroid complex contains the highest concentration of zinc in the eye, as measured using a wide variety of techniques in different species, including humans …”

Section: Zinc In the Normal Retinamentioning

confidence: 99%

“…Approximately 60% of the total body zinc content is found in skeletal muscle and 30% in bone mass . Of the remaining organs and tissues, the eye has an unusually high zinc content, with the highest amount of zinc concentrated in the retinal pigment epithelium (RPE) (RPE‐choroid, 292 ± 98.5 µg/g dry tissue), followed by the retina (123

\pm

62.2 µg g −1 dry tissue) . Zinc exists in the other ocular tissues, in the following (descending) order of content: the ciliary body, iris, optic nerve, sclera, cornea, and the lens …”

Section: Introductionmentioning

confidence: 99%

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Zinc Nutrition and Inflammation in the Aging Retina

Gilbert

Pető²,

Lengyel³

et al. 2019

Molecular Nutrition Food Res

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Zinc is an essential nutrient for human health. It plays key roles in maintaining protein structure and stability, serves as catalytic factor for many enzymes, and regulates diverse fundamental cellular processes. Zinc is important in affecting signal transduction and, in particular, in the development and integrity of the immune system, where it affects both innate and adaptive immune responses. The eye, especially the retina‐choroid complex, has an unusually high concentration of zinc compared to other tissues. The highest amount of zinc is concentrated in the retinal pigment epithelium (RPE) (RPE‐choroid, 292 ± 98.5 µg g−1 dry tissue), followed by the retina (123±62.2 µg g−1 dry tissue). The interplay between zinc and inflammation has been explored in other parts of the body but, so far, has not been extensively researched in the eye. Several lines of evidence suggest that ocular zinc concentration decreases with age, especially in the context of age‐related disease. Thus, a hypothesis that retinal function could be modulated by zinc nutrition is proposed, and subsequently trialled clinically. In this review, the distribution and the potential role of zinc in the retina‐choroid complex is outlined, especially in relation to inflammation and immunity, and the clinical studies to date are summarized.

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Section: Zinc In the Normal Retinamentioning

confidence: 98%

“…The retina–choroid complex contains the highest concentration of zinc in the eye, as measured using a wide variety of techniques in different species, including humans …”

Section: Zinc In the Normal Retinamentioning

confidence: 99%

\pm

62.2 µg g −1 dry tissue) . Zinc exists in the other ocular tissues, in the following (descending) order of content: the ciliary body, iris, optic nerve, sclera, cornea, and the lens …”

Section: Introductionmentioning

confidence: 99%

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Zinc Nutrition and Inflammation in the Aging Retina

Gilbert

Pető²,

Lengyel³

et al. 2019

Molecular Nutrition Food Res

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“…With respect to drugs or other exogenous analytes, “on‐tissue” or “under‐tissue” micropipetting strategies are also difficult to accurately reflect the similar drug status within native tissue, where desorption/ionization is being processed under the complicated suppression by cells and extracellular matrix (ECM). Apart from parallel cryo‐sections, tissue homogenate is another type of surrogate used for representing the analyte's surrounding environment . Compared with standard‐spiked native tissue sections, analytes have much more in‐depth incorporation and uniform distribution .…”

Section: Introductionmentioning

confidence: 99%

“…Apart from parallel cryo-sections, tissue homogenate is another type of surrogate used for representing the analyte's surrounding environment. [26][27][28][29][30][31][32][33] Compared with standard-spiked native tissue sections, analytes have much more in-depth incorporation and uniform distribution. 34,35 Unfortunately, cells can seldomly survive under this violent homogenization process, 35 whereas the existence of cells should also contribute as the real impact factor for the analyte's desorption/ionization.…”

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confidence: 99%

Fabrication of homogenous three‐dimensional biomimetic tissue for mass spectrometry imaging

Song

Sun

et al. 2019

J Mass Spectrom

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Reference samples are essential for mass spectrometric method optimization, data quality control, and target analyte quantitation. However, it is highly challenging to prepare an ideal homogeneous, standard‐spiked tissue sample for mass spectrometry imaging (MSI) research. Herein, we present a standard‐spiked 3D biomimetic tissue model fabricated with native cells, homogenate matrix, and biocompatible polymer. Unlike traditional homogenized tissue surrogates or those constructed with “on‐tissue” or “under‐tissue” micropipetting strategies, this simulated tissue shares both structural integrity of cells and homogeneous properties of matrix. As a result, analyte standards could undergo more in‐depth incorporation and has a more comparable native status with a real tissue. Series of tissue sections made from the 3D tissue model were proven to be feasible and useful for the parameter optimization, analyte quantitation, and calibration curve fitting for the air‐flow assisted desorption electrospray ionization MSI. Additionally, by analyzing the quality control model sections, we proposed a median principal component score calibration and demonstrated that this method can normalize instrumental fluctuations to stable levels in a large‐scale untargeted MSI experiments for the reliable metabolomic biomarker discovery. Thus, these results indicated that the standard‐spiked 3D biomimetic tissue has convincing significance in MSI analysis

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