Quantitative Study of Leydig Cell Populations in Mice Exposed to Low Doses of Cadmium

Blanco, Alfonso; Moyano, M.R.; Molina, AM; Blanco, Carmen; Flores-Acuña, Rafaela; García-Flores, Jose R; Espada, Mercedes; Monterde, J. G.

doi:10.1007/s00128-009-9700-1

Cited by 11 publications

(10 citation statements)

References 12 publications

(11 reference statements)

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“…Despite the reduction of Leydig cell cytoplasm in the GbE group compared with the control, these data are comparable with previous studies with rats [24,35,36]. The significant reduction of Leydig nuclear diameter that occurred in the cadmium treated group in accordance with the Leydig cell nuclear shrinkage and hyperchromatic images was reported by Blanco et al [37], who also found less circular Leydig cell nuclei. Ultrastructural observations, such as a diminished amount of endoplasmatic reticulum, mitochondria, and dense cytoplasmatic matrix, were found for Leydig cells.…”

Section: Discussionsupporting

confidence: 92%

Testicular Histomorphometry and Ultrastructure of Rats Treated with Cadmium and Ginkgo biloba

Predes

Monteiro

Matta

et al. 2010

Biol Trace Elem Res

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The aim of this study is to investigate the association of a single low dose of Cd and daily doses of Ginkgo biloba extract (GbE) on the testis and accessory glands of rats. The animals were treated with a single dose of 3 µmol/kg body weight of cadmium chloride (CdCl₂) and/or 100 mg/kg body weight of GbE. The plasma testosterone levels; corporal, testicular, and accessory glands weight; gonadosomatic index, volumetric proportion; and absolute volume of testicular components did not change after the treatments. CdCl₂ caused significant reduction in Leydig cells volume and altered Leydig cell morphology, as well as vacuolated Sertoli cells cytoplasm, irregular chromatin condensation of late spermatids, and modified acrosome formation. However, animals that received GbE did not show these alterations. The reversal of Cd-induced alterations by the extract is a strong indication that G. biloba is helpful in diminishing the effect of Cd toxicity.

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Section: Discussionsupporting

confidence: 92%

Testicular Histomorphometry and Ultrastructure of Rats Treated with Cadmium and Ginkgo biloba

Predes

Monteiro

Matta

et al. 2010

Biol Trace Elem Res

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“…Hew et al [23] showed that a single low cadmium dose causes failure of spermiation in rats. Also, a substantial number of morphological and biochemical changes have been described for testes, such as germinal cell loss [17], Leydig cell degeneration [24], testicular mass and dimensions decreasing [25], and increased oxidative processes accompanied by a reduced proportion of antioxidant enzymes [13,14]. Hew et al [23] demonstrated that rats injected with cadmium chloride in a single dose of 1 mg/kg body weight did not show any significant change on the seminiferous tubules morphology, under the light microscope, 48 h after metal exposition.…”

Section: Discussionmentioning

confidence: 99%

Protective Effect of Guaraná (Paullinia cupana var. sorbilis) Pre-treatment on Cadmium-Induced Damages in Adult Wistar Testis

Leite

Wada

Monteiro

et al. 2010

Biol Trace Elem Res

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Guaraná (Paullinia cupana) is an Amazonian plant. Its antioxidant potential was demonstrated to be due to the high polyphenol concentration. On the other hand, one of the mechanisms underlying cadmium-induced cellular damage is free radical mediated, resulting in increased oxidative processes. This study investigated P. cupana's potential to attenuate cadmium-induced damages in Wistar rat testis. Adult male Wistar rats were either pre-treated with 2 mg/g body weight (BW) of powdered P. cupana seed during 56 days and/or injected with cadmium chloride at a dose of 1.15 mg/kg BW. After cadmium exposition (48 h), testes samples were evaluated by histological and stereological analyses. Both groups exposed to cadmium presented evident morphological alterations relative to control animals. A few rodents showed massive cell death in the seminiferous epithelium and intertubular space, indicating that some animals are more sensitive to cadmium. Despite the alterations observed in both groups, pre-treatment with P. cupana was effective in attenuating morphological changes in Leydig cells, as well as reducing inflammatory response, relative to animals exclusively exposed to the metal. Animals treated only with P. cupana presented a significant increase in plasma testosterone levels and a significant increase in volumetric proportions of seminiferous tubules, which are indicative of spermatogenic stimulation.

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“…Previous studies by our research team have enabled us to confirm that exposure to compounds with a disruptor activity cause degenerative processes at a gonad level (BLaNCO et al, 2007;BLaNCO et al, 2009;PatrIgNaNI et al, 2014). We have also seen how BPa triggers important effects on ovaries and testicles, which could interfere with fertility (MOLINa et al, 2013;LOra et al, 2016), in addition to modifying other systems, such as the musculoskeletal system (gIMéNez et al, 2016).…”

Section: Resultsmentioning

confidence: 74%

Histopathological study of the Achilles tendon after exposure to bisphenol-A and its subsequent treatment with platelet growth factors

Bellido¹,

Córdoba²,

Abellán³

et al. 2018

Vet. arhiv

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Bisphenol-a is a well-known compound with estrogenic activity, which is produced in tons world-wide and is abundantly used as a component of food containers, so that it frequently migrates into food and appears as an environmental pollutant. this study aimed to assess the effects of Bisphenol-a at the tendon level, possible reversal after it is withdrawn from exposure, and its recovery after the application of plate-enriched plasma. For this purpose, 16 animals were randomly distributed to one of the experiment groups: control group, group treated with BPa (for 4 months), group BPa-withdrawal (treated for 2 months with BPa and two months without BPa), and platelet-rich plasma (PrP) group (treated for 2 months with BPa, when it was withdrawn and the PrP treatment began for 2 months). at the end of the treatments biopsy of the achilles tendon was carried out. the samples were analysed by optical and electron microscopy and immunohistochemistry. histopathology was a fundamental tool in the evaluation of the tendon after exposure to Bisphenol-a. Our results showed how, after withdrawal of BPa, there was recovery from the lesions caused, with diminution in the type III collagen fibres along with tendon vascularization processes; in the group to which PRP was applied, almost complete recovery from the lesions caused occurred, and the immunocytochemistry test was negative for the type III collagen fibres. Our results indicate that tendons were affected after BPA exposure, but that the lesions were partially reversible after BPa was withdrawn. PrP application enabled tendon lesions to recuperate and all signs of inflammation disappeared.

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Quantitative Study of Leydig Cell Populations in Mice Exposed to Low Doses of Cadmium

Cited by 11 publications

References 12 publications

Testicular Histomorphometry and Ultrastructure of Rats Treated with Cadmium and Ginkgo biloba

Testicular Histomorphometry and Ultrastructure of Rats Treated with Cadmium and Ginkgo biloba

Protective Effect of Guaraná (Paullinia cupana var. sorbilis) Pre-treatment on Cadmium-Induced Damages in Adult Wistar Testis

Histopathological study of the Achilles tendon after exposure to bisphenol-A and its subsequent treatment with platelet growth factors

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