Quantitative study of Babesia bovis infection in beef cattle from São Paulo state, Brazil

Bilhassi, T. B.; Oliveira, Henrique Nunes de; Ibelli, A. M. G.; Giglioti, Rodrigo; Regitano, Luciana Correia de Almeida; Oliveira-Sequeira, T.C.G.; Bressani, Flavia A.; Junior, Wilson Malagó; Resende, Flávio Dutra de; Oliveira, M. C. de S.

doi:10.1016/j.ttbdis.2013.11.002

Cited by 27 publications

(25 citation statements)

References 29 publications

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“…This genetic trait is transmitted to descendants, keeping a proportion of the blood lineage (Bock et al, 1997). This susceptibility can explain our results, as well as those of Bilhassi et al (2014) who observed, through qPCR for B. bovis, the greatest parasitaemia in B. t. taurus animals relative to B. t. indicus and its crossbreds, and the results of Ribeiro et al (1995) who observed low values of transplacental transmission of B. t. indicus in A. marginale.…”

Section: Discussionsupporting

confidence: 87%

Transplacental transmission of bovine tick-borne pathogens: Frequency, co-infections and fatal neonatal anaplasmosis in a region of enzootic stability in the northeast of Brazil

Costa

Magalhães

Oliveira³

et al. 2016

Ticks and Tick-borne Diseases

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Bovine tick-borne disease (TBD) constitutes a worldwide group of diseases that result in great losses for dairy and beef cattle. With regard to the epidemiological profile of the diseases, the importance of transplacental transmission is still not very well understood. The aim of this study was to determine the transplacental transmission of TBD agents (Anaplasma marginale, Babesia bovis and B. bigemina) in a herd of dairy cattle that had been naturally infected in an area of enzootic stability in northeastern Brazil. Blood for serology of the three agents was collected from cows within 120 days of gestation and serology, haemogram and nPCR assays were performed after birth. Blood was collected from the calves within 3h of birth, and haemogram and nPCR assays were performed in all animals. Pre-colostrum serology was achieved in 34 animals. The Student's t-test was used to compare the haemogram results between animals that were positive and negative for the haemoparasites. The cows were seropositive for all agents in at least one of the examinations. We detected 15 cases of vertical transmission of A. marginale, 4 of B. bovis and 2 of B. bigemina in the 60 cows. In infected animals, co-infection was detected for A. marginale and B. bovis in 1 of 60 calves, and a triple infection was detected in one other calf. Fatal neonatal anaplasmosis was observed in 1 of 15 calves, in which death occurred within 24h of birth. From the results, we concluded that transplacental transmission of TBD agents occurs, including in cases of co- and triple-infection. Such transplacental transmission can cause neonatal death, increasing the importance of this form of epidemiological transmission and suggesting its role as a cause of undiagnosed neonatal death.

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Section: Discussionsupporting

confidence: 87%

Transplacental transmission of bovine tick-borne pathogens: Frequency, co-infections and fatal neonatal anaplasmosis in a region of enzootic stability in the northeast of Brazil

Costa

Magalhães

Oliveira³

et al. 2016

Ticks and Tick-borne Diseases

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“…Additionally, Buling et al [22] with qPCR assays similar to those used in this work, confirmed that the analytical sensitivity was 100% higher than that in the ribosomal DNA-based assay. In correspondence, Billhassi et al [23], with qPCR, identified Nellore cattle infected with B. bovis that could not be previously detected by nPCR.…”

Section: Resultsmentioning

confidence: 99%

“…Initial qPCR reactions with positive controls as target DNA (ten replicates) were performed for each assay, and the resulting amplicons (88pb) were used to construct the standard curve for each assay. The amplicons were directly purified from the qPCR product using the commercial kit Pure Link PCR Purification Kit (Invitrogen) and cloned into the plasmid pGEM®-T Easy Vector Systems (Promega) (3000pb) as described by Bilhassi et al [23].…”

Section: Standard Curvesmentioning

confidence: 99%

“…However, the effectiveness of qPCR in the diagnosis and quantification of these protozoa in other host species has not been evaluated. In addition, the estimates of infection levels on carrier cattle are based on parasites/µL [21,23], which require an additional procedure enabling the transformation of qPCR outcomes into percent IRBC/mL. The present work was carried out with the objective of standardizing a SYBR Green based real-time PCR system for the detection and quantification of B. bovis and B. bigemina in carrier hosts, especially in water buffaloes.…”

Section: Introductionmentioning

confidence: 99%

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Standardization of a SYBR Green Based Real-Time PCR System for Detection and Molecular Quantification of Babesia bovis and B. bigemina in Water Buffaloes (Bubalus bubalis)

Obregón¹

2016

J Buf Sci

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Water buffalo (Bubalus bubalis) is a potential reservoir for Babesia bovis and B. bigemina in tropical regions, but the epidemiological evidence of their reservoir competence is limited, especially due to the lack of diagnostic tests capable of detecting and quantifying the low-level parasitemia present in the carrier animals. In this paper we present the standardization process of a SYBR Green based real-time PCR system (qPCR), consisting of two single qPCR assays, for the detection and quantification of B. bovis and/or B. bigemina. Both assays were optimized in similar protocols, including reagent concentrations and thermocycling parameters, so it is possible its use as a multiple qPCR in a single run. Both single assays showed a suitable analytical performance, especially by allowing detection of a greater number of carrier animals when compared with nested PCR assays (nPCR) against a reference panel of 60 DNA samples extracted from blood of both, infected-and non-infected buffaloes. Furthermore, a mathematical algorithm to convert the qPCR outcomes in percent of infected red blood cell was used, and was found that the estimated parasitemia in carrier buffaloes within the reference sample panels were close to those described in carrier cattle. This method could be a useful tool for epidemiological studies on the participation of the bubaline specie in the epidemic process of bovine babesiosis.

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“…Differences in the susceptibility to hemoparasites have been verified within breeds, suggesting that it may be possible to select resistant phenotypes [7] [9] [10] [11]. The present study evaluated the TNFα, IL10, IFN-γ, IL12β, and iNOS transcripts levels in persistently infected animals presenting high or low levels of B. bovis and B. bigemina loads, aiming to elucidate better the immune mechanisms involved on babesial clearance.…”

Section: Introductionmentioning

confidence: 99%

Differential IL10 mRNA Profiles Associated to <i>Babesia bovis</i> and <i>B. bigemina</i> Infection Levels in Persistently Infected Animals

Bilhassi¹,

Giglioti²,

Okino³

et al. 2019

OJVM

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This work aimed to find quantitative phenotypic traits that can be used to discriminate the levels of resistance/susceptibility to B. bovis and B. bigemina in two groups of cattle presenting the highest (H) or lowest (L) infection levels and Rhipicephalus microplus ticks count. The animals were selected from a previous study of 50 Canchim (5/8 Charolais/zebu) heifers raised in an endemic area for these parasites. These animals were evaluated regarding their TNFα, IL10, IFN-γ, IL12 and iNOS mRNA levels. No differences were found between these groups regarding TNFα, IFN-γ, IL12β or iNOS transcripts. However, the IL10 transcripts were significantly higher in the H group compared to the L group. Moreover, significant correlation coefficients were observed between B. bovis loads and both IL10 and IFN-γ transcripts, while no correlations were found for B. bigemina loads and all tested immune-related transcripts, suggesting that differential IL10 mRNA profiles were closely associated to B. bovis loads. Our results have contributed to a better understanding of the immune responses against Babesia infection, as we demonstrated that the IL10 cytokine levels might also influence or be influenced by parasitemia levels in persistently infected animals.

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Quantitative study of Babesia bovis infection in beef cattle from São Paulo state, Brazil

Cited by 27 publications

References 29 publications

Transplacental transmission of bovine tick-borne pathogens: Frequency, co-infections and fatal neonatal anaplasmosis in a region of enzootic stability in the northeast of Brazil

Transplacental transmission of bovine tick-borne pathogens: Frequency, co-infections and fatal neonatal anaplasmosis in a region of enzootic stability in the northeast of Brazil

Standardization of a SYBR Green Based Real-Time PCR System for Detection and Molecular Quantification of Babesia bovis and B. bigemina in Water Buffaloes (Bubalus bubalis)

Differential IL10 mRNA Profiles Associated to <i>Babesia bovis</i> and <i>B. bigemina</i> Infection Levels in Persistently Infected Animals

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Quantitative study of Babesia bovis infection in beef cattle from São Paulo state, Brazil

Cited by 27 publications

References 29 publications

Transplacental transmission of bovine tick-borne pathogens: Frequency, co-infections and fatal neonatal anaplasmosis in a region of enzootic stability in the northeast of Brazil

Transplacental transmission of bovine tick-borne pathogens: Frequency, co-infections and fatal neonatal anaplasmosis in a region of enzootic stability in the northeast of Brazil

Standardization of a SYBR Green Based Real-Time PCR System for Detection and Molecular Quantification of Babesia bovis and B. bigemina in Water Buffaloes (Bubalus bubalis)

Differential IL10 mRNA Profiles Associated to &lt;i&gt;Babesia bovis&lt;/i&gt; and &lt;i&gt;B. bigemina&lt;/i&gt; Infection Levels in Persistently Infected Animals

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Differential IL10 mRNA Profiles Associated to <i>Babesia bovis</i> and <i>B. bigemina</i> Infection Levels in Persistently Infected Animals