Quantitative studies of blood and bone marrow neutrophils in normal mice

Chervenick, Paul A.; Boggs,; Marsh, JC; Cartwright, G. E.; Mm, Wintrobe

doi:10.1152/ajplegacy.1968.215.2.353

Cited by 218 publications

(112 citation statements)

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“…Our results differ from many of these studies in that CS, a natural Gc, was administered in vivo to mice continuously for a period of 3 days, with Gc levels that were consistent with natural models of stress. Another difference between this study and previous studies was the use of mature bone marrow granulocytes for functional analysis instead of blood or peritoneal neutrophils (39,40). Our studies indicated that chemotaxis, phagocytosis, and superoxide production were unaffected or slightly enhanced by in vivo CS treatment.…”

Section: Discussionmentioning

confidence: 58%

Natural glucocorticoids induce expansion of all developmental stages of murine bone marrow granulocytes without inhibiting function

Trottier

Newsted

King

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

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Natural glucocorticoids (Gc) produced during stress have profound effects on the immune system. It is well known that Gc induce apoptosis in precursor T and B cells, markedly altering lymphopoiesis. However, it has been noted that marrow myeloid cells expanded both in proportion and absolute numbers in the mouse after Gc exposure. Mice were implanted with a corticosterone (CS) tablet that increased serum Gc and caused atrophied thymuses, both classic signs of activation of the stress axis. Blood neutrophil counts were elevated (4.8؋), whereas lymphocyte counts declined. Flow cytometric analysis of the marrow revealed that the phenotypic distribution of the various major classes of cells was shifted by Gc exposure. As expected, marrow lymphocyte numbers declined >40% after 3 days of exposure to Gc. Conversely, in the myeloid compartment, both monocytes and granulocytes increased in number by >40%. Further, all granulocyte developmental stages showed large increases in both total number and percentage of cells. To investigate the functional capacity of mature granulocytes from Gc-treated mice, an improved granulocyte isolation method was developed. Gc exposure had little effect on the ability of granulocytes to produce superoxide or undergo chemotaxis or phagocytose bacteria. These results indicate that Gc treatment shifts bone marrow composition and provides evidence that granulocytes and their progenitors are selectively preserved under stressful conditions without losing function.granulopoiesis ͉ lymphopoiesis

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Section: Discussionmentioning

confidence: 58%

Natural glucocorticoids induce expansion of all developmental stages of murine bone marrow granulocytes without inhibiting function

Trottier

Newsted

King

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

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“…Blood drawn from an artery tends to have a lower neutrophil concentration than blood drawn simultaneously from a vein or capillary source. [21][22][23] This difference is likely related to the pulsatile flow of arterial blood, 'pushing' the larger cells away from the rapidly flowing central stream and toward the arterial walls. Because of this, the central stream of arterial blood has a lower concentration of neutrophils.…”

Section: Neutrophil Counts Of Neonates N Schmutz Et Almentioning

confidence: 99%

Expected ranges for blood neutrophil concentrations of neonates: the Manroe and Mouzinho charts revisited

et al. 2008

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“…For calculation of the number of ASCs in the total BM, the number of ASCs per million cells was multiplied by the number of cells recovered from both femurs and tibias and by a coefficient of 5.3 (43).…”

Section: Enumeration Of Ascs By Elispotmentioning

confidence: 99%

Reduced Ability of Neonatal and Early-Life Bone Marrow Stromal Cells to Support Plasmablast Survival

Pihlgren

Friedli

Tougne

et al. 2006

The Journal of Immunology

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In human infants (<1 year), circulating IgG Abs elicited in response to most T-dependent Ags rapidly decline and return to baseline within a few months after immunization for yet-unknown reasons. In mice immunized between 1 and 4 wk of age, a limited establishment of the bone marrow (BM) pool of long-lived plasma cells is observed. In this study, we show that tetanus toxoid (TT)-specific plasmablasts generated in the spleen are efficiently attracted in vitro and in vivo toward early-life BM stromal cells, which express adult levels of CXCL12. Similarly, adoptively transferred TT plasmablasts efficiently reach the BM compartment of 2-wk-old and adult mice. In contrast, TT plasmablasts fail to persist in the early-life BM compartment, as indicated by the persistence of a significantly lower number of TT plasmablasts in the early-life compartment than in the adult BM compartment 48 h after transfer. This limited persistence is associated with an increased rate of in vivo apoptosis of TT-specific plasmablasts that have reached the early-life BM and with a significantly lower survival rate of TT-specific plasmablasts cocultured on early-life BM stromal cells compared with adult BM stromal cells. Thus, early-life BM stromal cells fail to provide the molecular signals that support plasmablast survival and differentiation into surviving plasma cells.

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Quantitative studies of blood and bone marrow neutrophils in normal mice

Cited by 218 publications

References 0 publications

Natural glucocorticoids induce expansion of all developmental stages of murine bone marrow granulocytes without inhibiting function

Natural glucocorticoids induce expansion of all developmental stages of murine bone marrow granulocytes without inhibiting function

Expected ranges for blood neutrophil concentrations of neonates: the Manroe and Mouzinho charts revisited

Reduced Ability of Neonatal and Early-Life Bone Marrow Stromal Cells to Support Plasmablast Survival

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