Quantitative SERS Analysis of Azorubine (E 122) in Sweet Drinks

Peksa, Vlastimil; Jahn, Martin; Štolcová, L.; Schulz, Volker; Proška, Jan; Procházka, Marek; Weber, Karina; Cialla‐May, Dana; Popp, Jürgen

doi:10.1021/ac504254k

Cited by 110 publications

(53 citation statements)

References 24 publications

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“…Another key point of any SERS analytical application is SERS sensitivity of a SERS‐active substrate. Recently, we successfully used as‐prepared AuFON substrates for detection and quantification of azorubine dye in drinks when the high sensitivity was not required …”

Section: Resultsmentioning

confidence: 99%

Gold film over very small (107 nm) spheres as efficient substrate for sensitive and reproducible surface‐enhanced Raman scattering (SERS) detection of biologically important molecules

Štolcová

Peksa

Proška

et al. 2017

J Raman Spectroscopy

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Gold plasmonic nanostructures with high sensitivity and spectral reproducibility are key components of molecular sensors based on surface‐enhanced Raman scattering (SERS). In this paper, we report a “bottom‐up” fabrication of gold “film over nanosphere” (FON) substrates for SERS sensing on the basis of very small (107 nm in diameter) polystyrene spheres coated with 20 nm of gold. To obtain close‐packed spheres in a large scale area, the self‐assembly at the water–air interface was found to be very efficient. Sensitivity and reproducibility of the AuFON substrates were tested using various molecular probes: 5,10,15,20‐tetrakis(1‐methyl‐4‐pyridyl)porphyrin, p‐aminothiophenol, and benzocaine. Relative standard deviation of SERS signal was found to be less than 20% confirming good spectral reproducibility especially using a 100× objective. Then, we demonstrated sensitive SERS detection of other biologically important molecules adsorbed on our AuFON substrates: thiolated‐polyA, protoporphyrin IX, and two alkaloids (nicotine and strychnine). Estimated limits of detection were 8.2 × 10−7 and 4.5 × 10−7 M (in order of 100 ng.ml−1) incubation concentration for strychnine and nicotine, respectively. Therefore, we conclude that the 20‐nm gold film deposited on polystyrene spheres of 107 nm in diameter provided both good spectral reproducibility and sensitivity. Their implementation into the SERS‐active system will enable to bring about new quantitative and analytical SERS applications.

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Section: Resultsmentioning

confidence: 99%

Gold film over very small (107 nm) spheres as efficient substrate for sensitive and reproducible surface‐enhanced Raman scattering (SERS) detection of biologically important molecules

Štolcová

Peksa

Proška

et al. 2017

J Raman Spectroscopy

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“…At high levels, these food additives are dangerous and toxic. The food and drink colorant azorubine or E 122 found in beverages is another example of one such additive, and SERS was used to quantify the levels with no sample preparation [175]. Various prohibited colorants, such as amaranth, erythrosine, lemon yellow, and sunset yellow, are also good candidates for SERS [176].…”

Section: Sers Of Small Moleculesmentioning

confidence: 99%

Fundamentals and applications of SERS-based bioanalytical sensing

et al. 2017

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Abstract:Plasmonics is an emerging field that examines the interaction between light and metallic nanostructures at the metal-dielectric interface. Surface-enhanced Raman scattering (SERS) is a powerful analytical technique that uses plasmonics to obtain detailed chemical information of molecules or molecular assemblies adsorbed or attached to nanostructured metallic surfaces. For bioanalytical applications, these surfaces are engineered to optimize for high enhancement factors and molecular specificity. In this review we focus on the fabrication of SERS substrates and their use for bioanalytical applications. We review the fundamental mechanisms of SERS and parameters governing SERS enhancement. We also discuss developments in the field of novel SERS substrates. This includes the use of different materials, sizes, shapes, and architectures to achieve high sensitivity and specificity as well as tunability or flexibility. Different fundamental approaches are discussed, such as label-free and functional assays. In addition, we highlight recent relevant advances for bioanalytical SERS applied to small molecules, proteins, DNA, and biologically relevant nanoparticles. Subsequently, we discuss the importance of data analysis and signal detection schemes to achieve smaller instruments with low cost for SERS-based point-of-care technology developments. Finally, we review the main advantages and challenges of SERS-based biosensing and provide a brief outlook.

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“…SERS applications span very diverse fields, in continuous expansion. One can mention the field of optical plasmonic sensors that comprises for instance the detection of explosives and warfare agents, food additives or contaminants, biological species, pigments in art preservation, and drugs in forensic science …”

Section: Introductionmentioning

confidence: 99%

SERS detection of food contaminants by means of portable Raman instruments

Pilot

2018

J Raman Spectroscopy

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Surface‐enhanced Raman scattering (SERS) has been emerging as a powerful tool for the detection of a variety of analytes due to its very high sensitivity and fingerprinting recognition capabilities. Technological progresses in the equipment for Raman analysis are contributing to its transition from a technically demanding research method to a more widely available analytical technique. In particular, the commercialization of portable or handheld instruments has opened up the possibility of performing in situ analysis. In this review, a selection of the SERS substrates that are expected to be more suitable for use in combination with portable instruments is presented: Substrates are compared, for example, in terms of performance, reproducibility, ease of fabrication, and surface area. Moreover, this paper provides a survey of the current diffusion of portable Raman instruments in the SERS detection of food contaminants: The investigation of several analytes is summarized (mainly toxins, virus, bacteria, pesticides, forbidden food dyes, and preservatives), reporting on the limits of detection and on the eventual coupling with concentration or separation techniques. A brief perspective on possible future developments of the SERS detection with portable instruments is also provided.

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Quantitative SERS Analysis of Azorubine (E 122) in Sweet Drinks

Cited by 110 publications

References 24 publications

Gold film over very small (107 nm) spheres as efficient substrate for sensitive and reproducible surface‐enhanced Raman scattering (SERS) detection of biologically important molecules

Gold film over very small (107 nm) spheres as efficient substrate for sensitive and reproducible surface‐enhanced Raman scattering (SERS) detection of biologically important molecules

Fundamentals and applications of SERS-based bioanalytical sensing

SERS detection of food contaminants by means of portable Raman instruments

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