Quantitative SEM analysis of injury to the endothelium of rabbit aorta and carotid artery during experimental atherosclerosis

Zaikina, O.E.; Dolgov, V V; Ivanov, Vladimir N.; Bondarenko, M. F.; Repin, V. S.

doi:10.1016/0021-9150(82)90180-0

Cited by 18 publications

(3 citation statements)

References 18 publications

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“…In our studies, we found that there is a significant increase in argyrophilic cells in diabetic animals, particularly after 5-week duration of diabetes, showing phasic process. Recently we have found phasic structural changes of endothelial integrity in rabbit aorta and carotid artery, during early stages of experimen-tal hypercholesterolaemia [24]. The presence of craters, as has been suggested previously [25], occurs as the result of weakening of intercellular junctions.…”

Section: Discussionsupporting

confidence: 66%

Aortic endothelium of alloxan diabetic rabbits: A quantitative study using scanning electron microscopy

et al. 1982

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Scanning electron microscope studies of the aorta and other major arteries have been performed in alloxan-induced diabetic rabbits. After 5 weeks, a variety of structural abnormalities of the endothelial lining were detected including a significant increase in the number of argyrophilic cells and an increased number of craters or openings in the endothelial junctional region. Evidence of more extensive micro-damage was present after 5 months duration of diabetes. These zones with structural changes in the endothelial lining of major vessels seem to be areas of high predilection to atherosclerosis in diabetes.

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Section: Discussionsupporting

confidence: 66%

Aortic endothelium of alloxan diabetic rabbits: A quantitative study using scanning electron microscopy

et al. 1982

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“…In humans and other mammals, the endothelial surface changes characterized by SEM include surface irregularities, due to the underlying accumulation of foam cells, erythrocytes, and platelets, monocyte adhesion, endothelial cell proliferation, and endothelial erosions (Davies et al, 1976;Goode et al, 1977;Weber et al, 1977;Zaikina et al, 1982;Pasquinelli et al, 1989;Song & Roach, 1998;Walski et al, 2002;Kamari et al, 2008;Congiu et al, 2010;Dell'Orbo et al, 2010). Similar changes have been observed in pigeons and Japanese quails (Taylor & Lewis 1986;Casale et al, 1992).…”

Section: Discussionmentioning

confidence: 99%

Characterization and classification of psittacine atherosclerotic lesions by histopathology, digital image analysis, transmission and scanning electron microscopy

Beaufrère¹,

Nevarez²,

Holder

et al. 2011

Avian Pathology

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Atherosclerosis is a degenerative and inflammatory vascular disease characterized in mammals and birds by the accumulation of inflammatory cells, lipids, calcium, and formation of large fibrofatty lesions within the intima of arteries resulting in the disorganization of the arterial wall and stenosis of the lumen. Despite the high incidence of atherosclerosis in parrots and the high number of case reports, there are few pathologic investigations and the ultrastructural study of the lesions has not been documented. Sixty-three major arteries were collected from 24 psittacine birds of 11 species during routine post-mortem examinations. Samples from the major arteries were fixed in 2% paraformaldehyde and 1.25% glutaraldehyde, and processed for transmission electron microscopy (TEM) and scanning electron microscopy (SEM). Additional samples were fixed in 10% formalin and embedded in paraffin for histological examination. Additional histochemical stains for calcium, elastic fibres, and lipid were performed. Toluidine blue-stained 0.5 mm-thick resin sections were also obtained. Digital image analysis was performed to provide objective quantitative information on the different lesions. The histopathology and ultrastructure of psittacine atherosclerosis were found to be similar to other avian and mammalian species. Seven lesion types could be described, which were similar to the human classification system. Digital image analysis, TEM, and SEM helped to further describe the lesions and refine the classification system. TEM findings were similar to other avian and mammalian species with the notable presence of macrophage-derived and smooth muscle cellderived foam cells and extracellular lipid. SEM revealed various stages of endothelial surface defects and, occasionally, adherent blood cells.

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“…The variation of serum lipid levels on hyperlipidemia in rabbits induced by cholesterol diets were reported by Kato (8), Nishimura (9) and others (5-7). The morphology of arterial lesions in this animal model have been studied using a light microscope (LM) (10), a transmission electron microscope (TEM) (2) and a scanning electron microscope (SEM) (3,4,8,9,11). In recent studies, the importance of hyperlipidemia and atherosclerosis in animals as experimental models for human disease was revaluated because of the increase in ischemic disease in humans and the new findings about the relationship between atherosclerosis and lipid metabolism in the body.…”

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Experimental hyperlipidemia and atherosclerosis induced by cholesterol diet in SPF Japanese white rabbits.

et al. 1983

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Abstract-Experimental hyperlipidemia and atherosclerosis induced by a cholesterol diet in SPF male and female rabbits (JW/KBL) were investigated by the determination of the lipid contents of the plasma, liver and thoracic aorta; determination of morphological changes of the aortic arch by head angiography; and computer tomography of the brain. Rabbits were fed the diet that contained 1 % cholesterol for eight weeks. The plasma lipid levels began to rise from two weeks after the cholesterol dietwas started, reached the peak four to six weeks later, and then fell in both males and females at eight weeks. The cholesterol of the high density lipoprotein in male rabbit plasma was slightly increased by the cholesterol diet, but not in female rabbits. An increase in the total cholesterol (TC) and triglyceride contents of the liver and an increase in the TC and phospholipid contents of the thoracic aorta were observed at the eighth week. Histological examination of the aortic arch showed marked lipid vacuoles under the endothelial cells, noticeable lipid inclusions in the smooth muscle cells of the intima and granular prominences on the internal surface of the aorta. Head angiography of rabbits fed the cholesterol diet revealed a constriction of the lumina of several arteries due to the lipid depositions. These results suggest that hyperlipidemia and atherosclerosis can be produced at the eighth week using SPF rabbits fed on a cholesterol diet.

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Quantitative SEM analysis of injury to the endothelium of rabbit aorta and carotid artery during experimental atherosclerosis

Cited by 18 publications

References 18 publications

Aortic endothelium of alloxan diabetic rabbits: A quantitative study using scanning electron microscopy

Aortic endothelium of alloxan diabetic rabbits: A quantitative study using scanning electron microscopy

Characterization and classification of psittacine atherosclerotic lesions by histopathology, digital image analysis, transmission and scanning electron microscopy

Experimental hyperlipidemia and atherosclerosis induced by cholesterol diet in SPF Japanese white rabbits.

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