Quantitative <scp>PCR</scp> of string‐test collected gastric material: A feasible approach to detect <i>Helicobacter pylori</i> and its resistance against clarithromycin and levofloxacin for susceptibility‐guided therapy

Han, Xinyuan; Yu, Xiqiu; Gao, Xiaojuan; Wang, Xiangyu; Tay, Chin Yen; Wei, Xiaolan; Lai, Bing; Marshall, Barry J.; Zhang, Xiuming; Chua, Eng Guan

doi:10.1111/hel.12985

Cited by 6 publications

(1 citation statement)

References 34 publications

(60 reference statements)

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“…They demonstrated that there was a concordance rate of 95.9% between such a string-qPCR and 13 C-urea breath test, an accurate non-invasive method widely used in clinical practice for diagnosis of H. pylori infection. Moreover, they reported a high eradication rate of 91.8% with genotypic resistancetailored bismuth quadruple therapy [82]. The value of the string test coupled with RT-qPCR for detecting H. pylori resistance to clarithromycin and levofloxacin has also been validated in urban China in a recent multicenter, cross-sectional study [83].…”

Section: Novel Techniques For Collecting Specimens For Genotypic-base...mentioning

confidence: 99%

Rapid Non-Invasive Techniques for Detecting Antibiotic Resistance in Helicobacter pylori: An Update and Clinical Applications

Zhou,

Zhang,

Qing-Jiao Liao

et al. 2024

Towards the Eradication of Helicobacter Pylori Infection - Rapid Diagnosis and Precision Treatment

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The global prevalence of Helicobacter pylori infection remains high, posing a significant health challenge worldwide. The efficacy of anti-H. pylori antibiotic-based regimens is compromised by the increasing antibiotic resistance in H. pylori. Thus, it is important to detect antibiotic resistance in H. pylori to ensure appropriate and effective treatment strategies. Currently, conventional culture-based methods are used for detecting antibiotic resistance (so-called phenotypic resistance) in H. pylori, but these methods are tedious and time-consuming (at least 72 h) and rely on the successful culture of H. pylori. Over the past decade, emerging genotypic or molecular techniques based on polymerase chain reaction or gene sequencing of DNA extracted from cultured H. pylori cells or H. pylori-containing specimens, such as gastric biopsy, stool, or saliva, have been developed to detect antibiotic resistance (so-called genotypic resistance) in H. pylori. These methods are rapid (usually within 4 h), non- or minimally invasive, cost-effective, and highly reproducible. Moreover, they can detect heteroresistant strains, enabling tailored therapy. The development and implementation of molecular techniques have significantly improved the accuracy and speed of identifying antibiotic resistance in H. pylori, allowing for more effective and personalized treatment strategies.

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Section: Novel Techniques For Collecting Specimens For Genotypic-base...mentioning

confidence: 99%

Rapid Non-Invasive Techniques for Detecting Antibiotic Resistance in Helicobacter pylori: An Update and Clinical Applications

Zhou,

Zhang,

Qing-Jiao Liao

et al. 2024

Towards the Eradication of Helicobacter Pylori Infection - Rapid Diagnosis and Precision Treatment

View full text Add to dashboard Cite

show abstract

Detection of Helicobacter pylori Infection in Human Gastric Fluid Through Surface-Enhanced Raman Spectroscopy Coupled With Machine Learning Algorithms

Tang,

Li,

Liu

et al. 2024

Laboratory Investigation

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Multicentre, cross-sectional surveillance of Helicobacter pylori prevalence and antibiotic resistance to clarithromycin and levofloxacin in urban China using the string test coupled with quantitative PCR

Wang,

Li,

Tay

et al. 2024

The Lancet Microbe

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Quantitative PCR of string‐test collected gastric material: A feasible approach to detect Helicobacter pylori and its resistance against clarithromycin and levofloxacin for susceptibility‐guided therapy

Abstract: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 6 publications

References 34 publications

Rapid Non-Invasive Techniques for Detecting Antibiotic Resistance in Helicobacter pylori: An Update and Clinical Applications

Rapid Non-Invasive Techniques for Detecting Antibiotic Resistance in Helicobacter pylori: An Update and Clinical Applications

Detection of Helicobacter pylori Infection in Human Gastric Fluid Through Surface-Enhanced Raman Spectroscopy Coupled With Machine Learning Algorithms

Multicentre, cross-sectional surveillance of Helicobacter pylori prevalence and antibiotic resistance to clarithromycin and levofloxacin in urban China using the string test coupled with quantitative PCR

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