Quantitative sandwich ELISA for the determination of fish in foods

Optimisation of an extraction technique of fish allergens suitable for detection and diagnosis

“…The remaining extracts lacked reactivity around 16 kDa. Faeste et al (2008) reported a protein of about 16 kDa which was recognised on Western blot by the polyclonal anti-cod parvalbumin antibody in more than 20 fish species. Some explanations for the observation are conceivable, either this band represented another parvalbumin complex or it possibly cross-reacted with the anti-cod parvalbumin IgG.…”

Rapid direct detection of the major fish allergen, parvalbumin, by selected MS/MS ion monitoring mass spectrometry

“…Several polyclonal and monoclonal antibodies have been development [18][19][20]. However, the limiting factors of these techniques are the availability of an universal antibody or a combination of antibodies that covers all β-PRVBs isoforms, combined with the cross-reaction problems and the alteration of antibody binding in whose foodstuffs that were subjected to heating or technological food processing.…”

Development of a real-time PCR system for the detection of the potential allergen fish in food

“…For detection of fish allergens in food, enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) technology are used most widely [7][8][9][10][11]. The majority of ELISA systems are based on the detection of the major fish allergenic protein, parvalbumin [8].…”

“…The majority of ELISA systems are based on the detection of the major fish allergenic protein, parvalbumin [8]. Compared to proteins, DNA is more stable against manufacturing processes.…”