Quantitative resistance to Botrytis cinerea from Solanum neorickii

Abstract

Tomato (Solanum lycopersicum) is susceptible to gray mold (Botrytis cinerea). Quantitative resistance to B. cinerea was previously identified in a wild relative, S. neorickii G1.1601. The 122 F 3 families derived from a cross between the susceptible S. lycopersicum cv. Moneymaker and the partially resistant S. neorickii G1.1601 were tested for susceptibility to B. cinerea using a stem bioassay. Three putative quantitative trait loci (pQTL) were detected: pQTL3 and pQTL9 reducing lesion growth (LG) and pQTL4 reducing disease incidence (DI). For each pQTL, a putative homologous locus was identified recently in another wild tomato relative, S. habrochaites LYC4. pQTL3 was confirmed by assessing disease resistance in BC 3 S 1 and BC 3 S 2 progenies of S. neorickii G1.1601. pQTL4 was not statistically confirmed but the presence of the S. neorickii resistance allele reduced DI in all three tested populations. The reduction in LG of pQTL9 was not confirmed but rather, this locus conferred a reduced DI, similar to observations in the QTL study using S. habrochaites. The results are discussed in relation to other disease resistance loci identified in studies with other wild tomato relatives.

Domesticated tomatoes are more vulnerable to negative plant–soil feedbacks than their wild relatives

“…Because the impact of soil associations on plant performance is the net effect of traits that both suppress (Bai & Lindhout, 2007). Thus, wild tomatoes often exhibit greater disease resistance when compared with domesticated lines (Finkers et al, 2008(Finkers et al, , 2007ten Have, van Berloo, Lindhout, & van Kan, 2007), as well as showing large-scale genetic differences based on comparative transcriptomics among the two groups (Koenig et al, 2013). Domestication-mediated differences in beneficial associations (e.g.…”

Quantitative Resistance: More Than Just Perception of a Pathogen

“…Additionally, the term partial resistance has an implication that total resistance is an achievable state, via biotechnology or evolution, which may not be possible for some pathogens. Illustrating the difficulty with the partial versus total resistance terminology is a qualitative resistance to B. cinerea within Solanum that is actually generated by a polygenic quantitative resistance architecture (Finkers et al, 2007a(Finkers et al, , 2007b(Finkers et al, , 2008.…”

“…Mapping of B. cinerea quantitative resistance loci within multiple plant species shows that the identified loci are highly dependent upon the specific pathogen genotype utilized and typically not broad spectrum (Denby et al, 2004;Rowe and Kliebenstein, 2008;Rowe et al, 2010;Corwin et al, 2016a;Finkers et al, 2007bFinkers et al, , 2008Zhang et al, 2016). An isolate dependency on the identification of quantitative disease resistance has also been found for the generalist S. sclerotiorum when studying sunflower (Helianthus annuus; Davar et al, 2011).…”

Complex Genetics Control Natural Variation inArabidopsis thalianaResistance toBotrytis cinerea

“…While understanding these isolate-specific, qualitative interactions provides valuable contributions to both applied and general understanding of plant defense, interest has grown in discovering the bases of ''partial'' or quantitative resistance, with the goal of developing durable resistance to diverse pathogens (Niks and Rubiales 2002). Naturally variable resistance to necrotrophic plant pathogens such as Botrytis cinerea, Alternaria brassisicola, Plectosphaerella cucurmerina, or Sclerotinia sclerotiorum appears to be quantitative and polygenic (Kim and Diers 2000;Micic et al 2004;Llorente et al 2005;Finkers et al 2007Finkers et al , 2008Maxwell et al 2007). However, the nature and extent of isolate-specific interaction between plants and necrotrophic pathogens is relatively unknown, and no qualitative naturally variable resistance genes effective against necrotrophic pathogens have been described ( Jones and Dangl 2006).…”