Quantitative resistance differences between and within natural populations of <i>Solanum chilense</i> against the oomycete pathogen <i>Phytophthora infestans</i>

Kahlon, Parvinderdeep S.; Verin, Mélissa; Hückelhoven, Ralph; Stam, Remco

doi:10.1002/ece3.7610

Cited by 8 publications

(26 citation statements)

References 73 publications

(120 reference statements)

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“…The wild tomato S. habrochaites shows resistance to Pinf, which is likely steered by polygenes [26]. Also in S. chilense Pinf resistance appears to be polygenic and of quantitative nature [53]. In S. habrochaites, multiple quantitative trait loci have been described on chromosomes 4, 5, and 11 [15,46].…”

Section: Origin Of Wild Tomato Species and Their Resistotypesmentioning

confidence: 99%

“…The relative abundance of epiphytic Proteobacteria and Firmicutes were altered by powdery mildew on E. japonicus [105]. Such observations raise questions about the role of phyllosphere microbiota in regulating PTI in wild tomato species, as large quantitative variations can be seen in resistance properties and PTI responses within single wild tomato species (Stam, Scheikl, and Tellier 2017, [53].…”

Section: Phyllosphere Microbiome Impacted By Tomato Species Across Ge...mentioning

confidence: 99%

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Distinct Phyllosphere Microbiome of Wild Tomato Species in Central Peru upon Dysbiosis

et al. 2022

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Plants are colonized by myriads of microbes across kingdoms, which affect host development, fitness, and reproduction. Hence, plant microbiomes have been explored across a broad range of host species, including model organisms, crops, and trees under controlled and natural conditions. Tomato is one of the world’s most important vegetable crops; however, little is known about the microbiota of wild tomato species. To obtain insights into the tomato microbiota occurring in natural environments, we sampled epiphytic microbes from leaves of four tomato species, Solanum habrochaites, S. corneliomulleri, S. peruvianum, and S. pimpinellifolium, from two geographical locations within the Lima region of Peru over 2 consecutive years. Here, a high-throughput sequencing approach was applied to investigate microbial compositions including bacteria, fungi, and eukaryotes across tomato species and geographical locations. The phyllosphere microbiome composition varies between hosts and location. Yet, we identified persistent microbes across tomato species that form the tomato microbial core community. In addition, we phenotypically defined healthy and dysbiotic samples and performed a downstream analysis to reveal the impact on microbial community structures. To do so, we compared microbial diversities, unique OTUs, relative abundances of core taxa, and microbial hub taxa, as well as co-occurrence network characteristics in healthy and dysbiotic tomato leaves and found that dysbiosis affects the phyllosphere microbial composition in a host species-dependent manner. Yet, overall, the present data suggests an enrichment of plant-promoting microbial taxa in healthy leaves, whereas numerous microbial taxa containing plant pathogens occurred in dysbiotic leaves.Concluding, we identify the core phyllosphere microbiome of wild tomato species, and show that the overall phyllosphere microbiome can be impacted by sampling time point, geographical location, host genotype, and plant health. Future studies in these components will help understand the microbial contribution to plant health in natural systems and can be of use in cultivated tomatoes.

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Section: Origin Of Wild Tomato Species and Their Resistotypesmentioning

confidence: 99%

Section: Phyllosphere Microbiome Impacted By Tomato Species Across Ge...mentioning

confidence: 99%

Distinct Phyllosphere Microbiome of Wild Tomato Species in Central Peru upon Dysbiosis

et al. 2022

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“…Such observations raise questions about the role of phyllosphere microbiota in regulating PTI in wild tomato species, as large quantitative variations can be seen in resistance properties and PTI responses within single wild tomato species (Stam, Scheikl, and Tellier 2017;Kahlon et al 2021).…”

Section: Discussionmentioning

confidence: 99%

“…The wild tomato S. habrochaites, shows resistance to Pinf, which is likely steered by polygenes (Copati et al 2019). Also in S. chilense Pinf resistance appears to be polygenic and of quantitative nature (Kahlon et al 2021). In S. habrochaites, multiple quantitative trait loci have been described on chromosome 4, 5 and 11 (Haggard, Johnson, and St. Clair 2015;Brouwer and St. Clair 2004).…”

Section: Declarations# Introductionmentioning

confidence: 99%

Distinct Phyllosphere Microbiome of Wild Tomato Species in Central Peru Upon Dysbiosis

Runge

Ventura

Kemen

et al. 2021

Preprint

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Plants are colonized by myriads of microbes across kingdoms, which affect host development, fitness and reproduction. Hence, plant microbiomes have been explored across a broad range of host species, including model organisms, crops and trees under controlled and natural conditions. Tomato is one of the world’s most important vegetable crops, however little is known about the microbiota of wild tomato species. To obtain insights into the tomato microbiota occurring in natural environments, we sampled epiphytic microbes from leaves of four tomato species, Solanum habrochaites, S. corneliomulleri, S. peruvianum and S. pimpinellifolium, from two geographical locations within the Lima region of Peru over two consecutive years. Here, a high-throughput sequencing approach was applied to investigate microbial compositions including bacteria, fungi and eukaryotes across tomato species and geographical locations. The phyllosphere microbiome composition varies between hosts and location. Yet, we identified persistent microbes across tomato species that form the tomato microbial core community. In addition, we phenotypically defined healthy and dysbiotic samples and performed a downstream analysis to reveal the impact on microbial community structures. To do so, we compared microbial diversities, unique OTUs, relative abundances of core taxa and microbial hub taxa, as well as co-occurrence network characteristics in healthy and dysbiotic tomato leaves and found that dysbiosis affects the phyllosphere microbial composition in a host species-dependent manner Yet, overall, the present data suggests an enrichment of plant-promoting microbial taxa in healthy leaves, whereas numerous microbial taxa containing plant pathogens occurred in dysbiotic leaves. Concluding, we identify the core phyllosphere microbiome of wild tomato species, and show that the overall phyllosphere microbiome can be impacted by sampling time point, geographical location, host genotype and plant health. Future studies in these components will help understand the microbial contribution to plant health in natural systems and can be of use in cultivated tomatoes.

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“…J41087, Manchester Organics, Runcorn, United Kingdom) or a water control 48 hours prior infection. The whole leaf area was spray infected with spore solutions of P. infestans isolate Pi100 (3000 sporangia/ml) as described (Kahlon et al ., 2021) or A. solani NL03003 (5000 spores/ml). Leaf infection frequencies (symptomatic leaflets/inoculated leaflets) were assessed 14 days after infection as described (Kahlon et al ., 2021).…”

Section: Methodsmentioning

confidence: 99%

Cross-family transfer of the Arabidopsis cell-surface immune receptor LORE to tomato confers sensing of 3-hydroxylated fatty acids and enhanced disease resistance

Eschrig,

Kahlon,

Agius

et al. 2024

Preprint

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Plant pathogens pose a high risk of yield losses and threaten food security. Technological and scientific advances have improved our understanding of the molecular processes underlying host-pathogen interactions, which paves the way for new strategies in crop disease management beyond the limits of conventional breeding. Cross-family transfer of immune receptor genes is one such strategy that takes advantage of common plant immune signaling pathways to improve disease resistance in crops. Sensing of microbe- or host damage-associated molecular patterns (MAMPs/DAMPs) by plasma membrane-resident pattern recognition receptors (PRR) activates pattern-triggered immunity (PTI) and restricts the spread of a broad spectrum of pathogens in the host plant. In the model plant Arabidopsis thaliana, the S-domain receptor-like kinase LIPOOLIGOSACCHARIDE-SPECIFIC REDUCED ELICITATION (AtLORE, SD1-29) functions as PRR, which senses medium chain-length 3-hydroxylated fatty acids (mc-3-OH-FAs), such as 3-OH-C10:0, and 3-hydroxyalkanoates (HAAs) of microbial origin to activate PTI. In this study, we show that ectopic expression of the Brassicaceae-specific PRR AtLORE in the solanaceous crop species Solanum lycopersicum cv. M82 leads to the gain of 3-OH-C10:0 immune sensing without altering plant development. AtLORE-transgenic tomato shows enhanced resistance against Pseudomonas syringae pv. tomato DC3000 and Alternaria solani NL03003. Applying 3-OH-C10:0 to the soil before infection induces resistance against the oomycete pathogen Phytophthora infestans Pi100 and further enhances resistance to A. solani NL03003. Our study proposes a potential application of AtLORE-transgenic crop plants and mc-3-OH-FAs as resistance-inducing bio-stimulants in disease management.

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Quantitative resistance differences between and within natural populations of Solanum chilense against the oomycete pathogen Phytophthora infestans

Cited by 8 publications

References 73 publications

Distinct Phyllosphere Microbiome of Wild Tomato Species in Central Peru upon Dysbiosis

Distinct Phyllosphere Microbiome of Wild Tomato Species in Central Peru upon Dysbiosis

Distinct Phyllosphere Microbiome of Wild Tomato Species in Central Peru Upon Dysbiosis

Cross-family transfer of the Arabidopsis cell-surface immune receptor LORE to tomato confers sensing of 3-hydroxylated fatty acids and enhanced disease resistance

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