Quantitative real-time RT-PCR measurement of cytokine mRNA expression in the skin of normal cats and cats with allergic skin disease

Taglinger, K; Nguyen, N. Van; Helps, Christopher R; Day, M. J.; Foster, Aiden P

doi:10.1016/j.vetimm.2007.11.014

Cited by 29 publications

(29 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Helminth infections in dogs, cats and mice evoke distinct immune responses (de Almeida Leal et al 2014) and each species has specific immune features in response to helminths or allergens (Dillon et al 2008;Taglinger et al 2007). In the feline allergic response, a clear Th2 immune response is lacking (Taglinger et al 2008). In dogs, the Th2 immune response is present in allergic responses; however, whether this evokes M2 macrophage activation is uncertain.…”

Section: Discussionmentioning

confidence: 99%

Adipose tissue macrophages in non-rodent mammals: a comparative study

et al. 2015

View full text Add to dashboard Cite

The stromal vascular fraction (SVF) of adipose tissue in rodents and primates contains mesenchymal stem cells and immune cells. SVF cells have complex metabolic, immune and endocrine functions with biomedical impact. However, in other mammals, the amount of data on SVF stem cells is negligible and whether the SVF hosts immune cells is unknown. In this study, we show that the SVF is rich in immune cells, with a dominance of adipose tissue macrophages (ATMs) in cattle (Bos primigenius taurus), domestic goat (Capra aegagrus hircus), domestic sheep (Ovis aries), domestic cat (Felis catus) and domestic dog (Canis familiaris). ATMs of these species are granulated lysosome-rich cells with lamellipodial protrusions and express the lysosome markers acid phosphatase 5 (ACP-5) and Mac-3/Lamp-2. Using ACP-5 and Mac-3/Lamp-2 as markers, we additionally detected ATMs in other species, such as the domestic horse (Equus ferus caballus), wild boar (Sus scrofa) and red fox (Vulpes vulpes). Feline and canine ATMs also express the murine macrophage marker F4/80 antigen. In the lean condition, the alternative macrophage activation marker CD206 is expressed by feline and canine ATMs and arginase-1 by feline ATMs. Obesity is associated with interleukin-6 and interferon gamma expression and with overt tyrosine nitration in both feline and canine ATMs. This resembles the obesity-induced phenotype switch of murine and human ATMs. Thus, we show, for the first time, that the presence of ATMs is a general trait of mammals. The interaction between the adipose cells and SVF immune cells might be evolutionarily conserved among mammals.

show abstract

Section: Discussionmentioning

confidence: 99%

Adipose tissue macrophages in non-rodent mammals: a comparative study

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Due to its rapid immunosuppressive effects and its action on T‐lymphocytes, Langerhans cells, mast cells and eosinophils (the cells involved in the pathogenesis of atopic dermatitis), ciclosporin has been used successfully in human and canine atopic dermatitis (Olivry and others 2002, Robson and Burton 2003, Guaguère and others 2004). The pathogenesis of feline atopic dermatitis has not been entirely investigated; some authors consider it to be similar to the human and canine form (Roosje and others 2000), while others obtained contradictory findings (Taglinger and others 2004). Clinical manifestations of feline allergic skin diseases classically include pruritus, leading to self‐inflicted alopecia and/or excoriations, miliary dermatitis and lesions of the EGC.…”

Section: Discussionmentioning

confidence: 99%

Prospective open pilot study on the use of ciclosporin for feline allergic skin disease

Noli¹,

Scarampella²

2006

J of Small Animal Practice

View full text Add to dashboard Cite

show abstract

“…For the feline housekeeping gene GAPDH, as well as feline IL-1, -6, -18, TNF-α and TGF-β1,primers of previously published sequences were used (Kipar et al, 2001;Van Nguyen et al, 2006;Taglinger et al, 2008). Primers for feline IL-2, IL-4, IFN-γ, MMP-2, -3, 13, TIMP-1, -2 and -3 were designed using Primer Express software (Applied Biosystems, Life Technologies), and BLAST searches performed to confirm gene specificity.…”

Section: 3real Time Pcrmentioning

confidence: 99%

Age- and gender-dependent myocardial transcription patterns of cytokines and extracellular matrix remodelling enzymes in cats with non-cardiac diseases

Fonfara

Hetzel

Hahn

et al. 2015

Experimental Gerontology

View full text Add to dashboard Cite

Background:Age, genderand systemic diseases all influence cardiac function and remodelling.In cats, age and genderassociated myocardial remodelling and the effect of systemic diseases on the myocardium have so far not been studied. The aim of the study was therefore to investigate whetherrelevant cytokines and extracellular matrix (ECM) remodellingenzymesare expressed in the myocardium of cats with non-cardiac diseasesand whether transcription levels are influenced by age and gender. Methods:The study was performed on myocardial samples from 26 cats aged between 2 and 19 years that had died with non-cardiac diseases. Seventeen cats were female (2 entire), nine were male (1 entire). Of these, nine cats were diagnosed with diseases unlikely to affect the myocardium (control cats). The remaining 17 cats suffered from diseases with likely systemic effects.All hearts were assessed for any pathological changes, and the myocardium was analysed for interleukin (IL)-1, -2, -4, -6, -18, tumour necrosis factor (TNF)-, interferon(IFN)-, transforming growth factor (TGF)-, matrix metalloproteinase (MMP)-2, -3, -13, tissue inhibitor of MMP (TIMP)-1, -2 and -3 transcription using quantitative RT-PCR assays. Results:Despite the absence of any histological evidence of myocardial damage,inflammation and fibrosis, the myocardium of all cats was found to constitutively transcribe cytokines and ECM remodelling enzymes, with generally higher mRNA concentrations in atria than in ventricles.Young and male cats exhibited higher transcription levels throughout the myocardium in comparison to older and female cats. Furthermore, age-associated transcription pattern differed between male and female cats. Conclusion:The constitutive transcription of ECM remodellingenzymes suggests continuous myocardial remodelling throughout the entire life of a cat. The myocardium of young and malecats appears to be in a pro-inflammatory state, whereas in older and female cats the myocardium exhibits a reduced inflammatory reaction to systemic disease.Age-associated cardiac remodelling seems to be influenced bynon-hormonal factors inmale and female cats. Abstract: Background: Age, gender and systemic diseases all influence cardiac function and remodelling. In cats, age and gender associated myocardial remodelling and the effect of systemic diseases on the myocardium have so far not been studied. The aim of the study was therefore to investigate whether relevant cytokines and extracellular matrix (ECM) remodelling enzymes are expressed in the myocardium of cats with non-cardiac diseases and whether transcription levels are influenced by age and gender. Methods: The study was performed on myocardial samples from 26 cats aged between 2 and 19 years that had died with non-cardiac diseases. Seventeen cats were female (2 entire), nine were male (1 entire). Of these, nine cats were diagnosed with diseases unlikely to affect the myocardium (control cats). The remaining 17 cats suffered from diseases with likely systemic effects. All hearts were assessed fo...

show abstract

Quantitative real-time RT-PCR measurement of cytokine mRNA expression in the skin of normal cats and cats with allergic skin disease

Cited by 29 publications

References 54 publications

Adipose tissue macrophages in non-rodent mammals: a comparative study

Adipose tissue macrophages in non-rodent mammals: a comparative study

Prospective open pilot study on the use of ciclosporin for feline allergic skin disease

Age- and gender-dependent myocardial transcription patterns of cytokines and extracellular matrix remodelling enzymes in cats with non-cardiac diseases

Contact Info

Product

Resources

About