Quantitative real‐time PCR primer design, cDNA amplification and sequence analysis from 22 genes mainly associated with lipid metabolism in Pekin (<i>Anas platyrhynchos</i>) and Muscovy (<i>Cairina moschata</i>) ducks

Herault, Frédéric; Robert, Estelle; Diot, Christian

doi:10.1111/j.1365-2052.2008.01732.x

Cited by 3 publications

(4 citation statements)

References 7 publications

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“…Expression of genes was analysed in Pekin and Muscovy duck livers by real‐time quantitative PCR (Q‐PCR) amplifications with SYBR‐Green® as described previously (Bourneuf et al. 2006) and using duck specific primers (Hérault et al. 2008).…”

Section: Methodsmentioning

confidence: 99%

“…Each gene‐specific primer pair was tested for PCR amplification efficiency, with cDNA dilutions from 1/2 to 1/2048. The efficiencies obtained were in a range of 99.8 ± 0.57% (Hérault et al. 2008).…”

Section: Methodsmentioning

confidence: 99%

“…2008). Q‐PCR was then performed in triplicate in an IQ‐cycler (BioRad) in the presence of 1X of IQ TM SYBR‐Green® Supermix (BioRad), 300 n m specific primers (Hérault et al. 2008), and 5 μl of 1/10 diluted cDNA in a 25‐μl total volume, and using a two‐step PCR protocol (95 °C for 2 min, followed by 45 cycles of 95 °C for 15 s and 59 °C for 45 s).…”

Section: Methodsmentioning

confidence: 99%

“…2002). Expression analyses were conducted by quantitative RT‐PCR using the primers developed previously by us (Hérault et al. 2008) on Pekin and Muscovy ducks fed ad libitum or overfed according to different protocols, to identify those with responses to overfeeding that were under the control of genetic effects.…”

Section: Introductionmentioning

confidence: 99%

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Liver gene expression in relation to hepatic steatosis and lipid secretion in two duck species

Herault¹,

Saez²,

Robert³

et al. 2010

Animal Genetics

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The susceptibility to development of hepatic steatosis is known to differ between Muscovy and Pekin ducks. Although some experiments were conducted to decipher these differences, few data have been produced to analyse the role of specific genes in this process. For this purpose, expression levels of genes involved in lipid (ATP citrate lyase, malic enzyme 1, fatty acid synthase, stearoyl-CoA desaturase 1, diacylglycerol O-acyl transferase 2, microsomal triglyceride transfer protein, apolipoprotein A1, apolipoprotein B, sterol regulatory element binding factor 1, hepatocyte nuclear factor 4, choline/ethanolamine phosphotransferase 1, carnitine palmitoyl transferase 1A, peroxisome proliferator-activated receptor alpha and sterol O-acyltransferase) and carbohydrate (activating transcription factor 4 or cAMP-response element binding protein, mitochondrial malate dehydrogenase 2 and carbohydrate responsive element binding protein) metabolism and in other functions were analysed in the liver of Pekin and Muscovy ducks fed ad libitum or overfed. A specific positive effect of feeding was observed on the expression of genes involved mainly in fatty acids and TG synthesis and glycolysis, and negative effect on genes involved in beta-oxidation. Interestingly, a strong species effect was also observed on stearoyl-CoA desaturase 1 and to a lesser extent on diacylglycerol O-acyl transferase 2 expression, leading to large differences in expression levels between Pekin and Muscovy overfed ducks, which could explain the difference in lipid metabolism and steatosis ability observed between the two duck species. These results should shed light on gene expression that might underlie susceptibility to hepatic steatosis in humans.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Liver gene expression in relation to hepatic steatosis and lipid secretion in two duck species

Herault¹,

Saez²,

Robert³

et al. 2010

Animal Genetics

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Current World Literature

2009

Current Opinion in Lipidology

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Growth, serum biochemistry, complement activity, and liver gene expression responses of Pekin ducklings to graded levels of cultured aflatoxin B1

Chen

Horn

Cotter³

et al. 2014

Poultry Science

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A 14-d study was conducted to evaluate the effects of cultured aflatoxin B1 (AFB1) on performance, serum biochemistry, serum natural antibody and complement activity, and hepatic gene expression parameters in Pekin ducklings. A total of 144 male Pekin ducklings were weighed, tagged, and randomly allotted to 4 dietary treatments containing 4 concentrations of AFB1 (0, 0.11, 0.14, and 0.21 mg/kg) from 0 to 14 d of age (6 cages per diet; 6 ducklings per cage). Compared with the control group, there was a 10.9, 31.7, and 47.4% (P < 0.05) decrease in cumulative BW gain with 0.11, 0.14, and 0.21 mg of AFB1/kg of diet, respectively, but feed efficiency was not affected. Increasing concentrations of AFB1 reduced cumulative BW gain and feed intake both linearly and quadratically, and regression equations were developed with r(2) ≥0.73. Feeding 0.11 to 0.21 mg of AFB1/kg reduced serum glucose, creatinine, albumin, total protein, globulin, Ca, P, and creatine phosphokinase linearly, whereas serum urea N, Cl, alkaline phosphatase, and aspartate amino transferase concentrations increased linearly with increasing AFB1 (P < 0.05). Additionally, 0.11 to 0.21 mg of AFB1/kg diets impaired classical and alternative complement pathways in the duckling serum when tested by lysis of rabbit, human type O, and horse erythrocytes, and decreased rabbit and horse agglutinins (P < 0.05). Liver peroxisome proliferator activated receptor α (PPARα) expression was linearly downregulated by AFB1 (P < 0.01). Results from this study indicate that for every 0.10 mg/kg increase in dietary AFB1, cumulative feed intake and BW gain decrease approximately 230 and 169 g per duckling from hatch to 14 d; and that AFB1 at very low concentrations can significantly impair liver function and gene expression, and innate immune dynamics in Pekin ducklings.

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Quantitative real‐time PCR primer design, cDNA amplification and sequence analysis from 22 genes mainly associated with lipid metabolism in Pekin (Anas platyrhynchos) and Muscovy (Cairina moschata) ducks

Cited by 3 publications

References 7 publications

Liver gene expression in relation to hepatic steatosis and lipid secretion in two duck species

Liver gene expression in relation to hepatic steatosis and lipid secretion in two duck species

Current World Literature

Growth, serum biochemistry, complement activity, and liver gene expression responses of Pekin ducklings to graded levels of cultured aflatoxin B1

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