Quantitative real-time PCR detection of oral Enterococcus faecalis in humans

Sedgley, Christine M.; Nagel, Aaron; Shelburne, Charles E.; Clewell, Don B.; Appelbe, Oliver K.; Molander, Anders

doi:10.1016/j.archoralbio.2004.10.017

Cited by 102 publications

(87 citation statements)

References 28 publications

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“…Data does suggest that the gelatin hydrolyzing activity is different from caseinase and also supported previous findings (30). Sedgley et al [33] found that gelatinase gene (gelE) was detected in all endodontic isolates of E. faecalis while expressed gelatinase activity was observed in two thirds of the isolates. These studies concluded that evidence of potential virulence determinants were identified in endodontic Enterococcus spp., specifically production of gelatinase and response to pheromones.…”

Section: Discussionsupporting

confidence: 79%

Phenotypic Detection of Virulence Traits and Antibiotic Susceptibility of Endodontic Enterococcus faecalis Isolates

Patidar¹,

Gupta²,

Singh³

2013

AJMR

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Enterococcus faecalis is a Gram-positive member of human gastrointestinal flora that is in recent years emerging as an important cause of endodontic infections. In this study, we have investigated the occurrence of virulence determinants and antibiotic susceptibility pattern of E. faecalis isolates, originating from root canals of apical periodontitis. Among 52 E. faecalis isolates, 32 (61.5%) isolates produced hemolysin on blood agar while all (100%) isolates showed hemolysin production on BHI-GA ((BHI medium supplemented with 1% glucose and 0.03% L-arginine), 18 (34.6%) produced gelatinase, 38 (73%) produced caseinase, no hemagglutination was observed in E. faecalis isolates, whereas all 52 (100%) resistant to serum and formed biofilm. Antibiotic susceptibility results showed that all (100%) E. faecalis isolates were susceptible to amoxicillin, amoxicillin/clavulanate, and vancomycin. Whereas, 32 (61.5%) E. faecalis isolates were resistant to chloramphenicol, 30 (57.6%) isolates were resistant to ciprofloxacin, 39 (75%) isolates were resistant to erythromycin, and 34 (65.3%) isolates were resistant to tetracycline. Multi-drug resistance was observed in 16 (30.7%) isolates of E. faecalis to chloramphenicol, ciprofloxacin, erythromycin and tetracycline antibiotics. These findings demonstrate the presence of putative virulence determinants in E. faecalis isolates originating from root canal and suggest amoxicillin, amoxicillin/clavulanate, and vancomycin as more effective than other antibiotics tested.

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Section: Discussionsupporting

confidence: 79%

Phenotypic Detection of Virulence Traits and Antibiotic Susceptibility of Endodontic Enterococcus faecalis Isolates

Patidar¹,

Gupta²,

Singh³

2013

AJMR

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“…Furthermore, since enterococci have been found in the oral environment (50,52,53), the usual location for S. gordonii (27), and conversely, oral streptococci have been identified in the gut environment (31), routinely inhabited by E. faecalis, the opportunity for cohabitation of the two organisms further supports the potential for genetic exchange.…”

Section: Vol 192 2010 Cam373-like Activity In S Gordonii 2543mentioning

confidence: 99%

A Genetic Determinant in Streptococcus gordonii Challis Encodes a Peptide with Activity Similar to That of Enterococcal Sex Pheromone cAM373, Which Facilitates Intergeneric DNA Transfer

et al. 2010

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Enterococcus faecalis strains secrete multiple peptides representing different sex pheromones that induce mating responses by bacteria carrying specific conjugative plasmids. The pheromone cAM373, which induces a response by the enterococcal plasmid pAM373, has been of interest because a similar activity is also secreted by Streptococcus gordonii and Staphylococcus aureus. The potential to facilitate intergeneric DNA transfer from E. faecalis is of concern because of extensive multiple antibiotic resistance, including vancomycin resistance, that has emerged among enterococci in recent years. Here, we characterize the related pheromone determinant in S. gordonii and show that the peptide it encodes, gordonii-cAM373, does indeed induce transfer of plasmid DNA from E. faecalis into S. gordonii. The streptococcal determinant camG encodes a lipoprotein with a leader sequence, the last 7 residues of which represent the gordonii-cAM373 heptapeptide SVFILAA. Synthetic forms of the peptide had activity similar to that of the enterococcal cAM373 AIFILAS. The lipoprotein moiety bore no resemblance to the lipoprotein encoded by E. faecalis. We also identified determinants in S. gordonii encoding a signal peptidase and an Eep-like zinc metalloprotease (lspA and eep, respectively) similar to those involved in processing certain pheromone precursors in E. faecalis. Mutations generated in camG, lspA, and eep each resulted in the ablation of gordonii-cAM373 activity in culture supernatants. This is the first genetic analysis of a potential sex pheromone system in a commensal oral streptococcal species, which may have implications for intergeneric gene acquisition in oral biofilms.

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“…Caracterizam-se por micro-organismos facultativos e catalase-negativos, incluindo diversas espécies que habitam o trato gastrointestinal e a cavidade oral de seres humanos [2][3][4] . O principal agente etiológico desse gênero, responsável por perdurar diversas infecções resistentes a antibióticos, como tetraciclina e gentamincina 5 , é o Enterococcus faecalis, inclusive, com capacidade de transmitir sua resistência antibiótica a outras espécies microbianas 6 . E. faecalis é encontrado em infecções endodônticas em Odontologia, comumente e em maior porcentagem em infecções persistentes, quando da comparação com infecções primárias.…”

Section: Introductionunclassified

Características microbiológicas, patogenicidade e viabilidade do Entercoccus faecalis e seu cultivo in vitro em pesquisas microbiológicas na área da Endodontia

Zandonà

Souza

2017

Rev. da Fac. de Odontologia, UPF

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Objetivo: o objetivo do trabalho foi revisar a literatura e descrever as características microbiológicas, patológi-cas e clínicas do Enterococcus faecalis na IntroduçãoBactérias e seus produtos desempenham um papel fundamental na indução e, principalmente, na perpetuação das alterações patológicas que acometem a polpa e os tecidos periapicais¹. Micro-organismos do gênero Enterococcus pertencem ao grupo de bactérias produtoras de ácido lático e são Gram--positivas. Caracterizam-se por micro-organismos facultativos e catalase-negativos, incluindo diversas espécies que habitam o trato gastrointestinal e a cavidade oral de seres humanos [2][3][4] . O principal agente etiológico desse gênero, responsável por perdurar diversas infecções resistentes a antibióticos, como tetraciclina e gentamincina 5 , é o Enterococcus faecalis, inclusive, com capacidade de transmitir sua resistência antibiótica a outras espécies microbianas 6 .

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Quantitative real-time PCR detection of oral Enterococcus faecalis in humans

Cited by 102 publications

References 28 publications

Phenotypic Detection of Virulence Traits and Antibiotic Susceptibility of Endodontic <i>Enterococcus </i><i>f</i><i>aecalis</i> Isolates

Phenotypic Detection of Virulence Traits and Antibiotic Susceptibility of Endodontic <i>Enterococcus </i><i>f</i><i>aecalis</i> Isolates

A Genetic Determinant in Streptococcus gordonii Challis Encodes a Peptide with Activity Similar to That of Enterococcal Sex Pheromone cAM373, Which Facilitates Intergeneric DNA Transfer

Características microbiológicas, patogenicidade e viabilidade do Entercoccus faecalis e seu cultivo in vitro em pesquisas microbiológicas na área da Endodontia

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