Quantitative Radioautographic Study on 3H-Leucine Uptake of Peri- and Tele-Insular Acinar Cells of the Pancreas in Normal and Streptozotocin-Diabetic Mice.

Abstract

The uptake of 3H-leucine by peri-and tele-insular acinar cells of the pancreas was analyzed quantitatively 60 min after the intraperitoneal injection of 3H-leucine in normal and streptozotocin-diabetic mice. A large tissue sample was analyzed and the grain density was counted with a color image analyzer equipped with a computer. The radioactivity was expressed as number of silver grains per acinar cell as well as per unit area of acinar tissue. In normal mice, the radioactivity in peri-insular acinar cells was 43% greater than in tele-insular acinar cells. The intercalated ducts associated with the former also showed a larger number of silver grains. In streptozotocin-diabetic mice, the uptake of 3H-leucine in peri-and teleinsular acinar cells was almost similar. The estimate of mean acinar cell profile area was 30% larger in the peri-insular acini of normal mice, while no difference was observed between the estimates of peri-and teleinsular acinar cells of diabetic mice.These results confirm previous reports that the B cell serection plays a certain role in the protein synthesis of pancreatic acinar cells, especially for the cells of peri-insular acini.

Intermediate Filament Keratins in The Exocrine Pancreas

“…While most of the studies showed a positive effect of insulin the results are complicated by changes in the precursor pool or changes in the levels of mRNAs. When protein synthesis in mice was quantitated by autoradiography, there was more incorporation into peri-insular acinar cells then into tele-insular acinar cells (7). This difference was lost following treatment with streptozotocin and the authors ascribed it to insulin.…”