Quantitative proteomics reveals the importance of nitrogen source to control glucosinolate metabolism in<i>Arabidopsis thaliana</i>and<i>Brassica oleracea</i>

Marino, Daniel; Ariz, Idoia; Lasa, Berta; Santamaría, Enrique; Fernández‐Irigoyen, Joaquín; González‐Murua, Carmen; Tejo, Pedro María Aparicio

doi:10.1093/jxb/erw147

Cited by 52 publications

(48 citation statements)

References 58 publications

(70 reference statements)

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“…On the other hand, the activation of GLS and GSH synthesis could also be part of the plant strategy to store N when cell NH 4 + availability is excessive. These results obtained in oilseed rape are in line with those reported in a recent study from our research group where we showed an increase in the synthesis of aliphatic and indolic GLS in shoots of A. thaliana and broccoli under mild ammonium stress [ 27 ]. Similarly, Zaghdoud et al (2016) [ 26 ] also observed higher levels of indolic GLS in broccoli plants under high ammonium stress associated to Trp accumulation.…”

Section: Discussionsupporting

confidence: 92%

“…Some studies reported no differences or even reduced levels of GLS in plants cultured with NH 4 + as sole N source [ 23 , 24 ], while other showed increased GLS levels in response to NH 4 + availability [ 25 , 26 ]. Recently, we also reported the activation of GLS metabolism in leaves of A. thaliana and broccoli plants grown under ammonium nutrition suggesting N management as a way to control GLS metabolism [ 27 ].…”

Section: Introductionmentioning

confidence: 99%

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Leaves play a central role in the adaptation of nitrogen and sulfur metabolism to ammonium nutrition in oilseed rape (Brassica napus)

et al. 2017

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BackgroundThe coordination between nitrogen (N) and sulfur (S) assimilation is required to suitably provide plants with organic compounds essential for their development and growth. The N source induces the adaptation of many metabolic processes in plants; however, there is scarce information about the influence that it may exert on the functioning of S metabolism. The aim of this work was to provide an overview of N and S metabolism in oilseed rape (Brassica napus) when exposed to different N sources. To do so, plants were grown in hydroponic conditions with nitrate or ammonium as N source at two concentrations (0.5 and 1 mM).ResultsMetabolic changes mainly occurred in leaves, where ammonium caused the up-regulation of enzymes involved in the primary assimilation of N and a general increase in the concentration of N-compounds (NH4 +, amino acids and proteins). Similarly, the activity of key enzymes of primary S assimilation and the content of S-compounds (glutathione and glucosinolates) were also higher in leaves of ammonium-fed plants. Interestingly, sulfate level was lower in leaves of ammonium-fed plants, which was accompanied by the down-regulation of SULTR1 transporters gene expression. ConclusionsThe results highlight the impact of the N source on different steps of N and S metabolism in oilseed rape, notably inducing N and S assimilation in leaves, and put forward the potential of N source management to modulate the synthesis of compounds with biotechnological interest, such as glucosinolates.Electronic supplementary materialThe online version of this article (10.1186/s12870-017-1100-9) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Leaves play a central role in the adaptation of nitrogen and sulfur metabolism to ammonium nutrition in oilseed rape (Brassica napus)

et al. 2017

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“…Supplying the two forms of nitrogen in a specific ratio will lead to the optimum growth; this finding has been reported in species such as apple (Malus domestica Borkh) [18,19], tomato (Solanum lycopersicum L.) [20,21], and rice (Oryza sativa L.) [22][23][24]. Predominant sources of ammonia N may also negatively affect the whole plant biomass deposition in some species, such as Arabidopsis thaliana [25,26]; while in another study, Arabidopsis plants displayed the potential of stimulating glucosinolate metabolism with ammonium nutrition [27]. An appropriate ammonium-nitrate ratio can enhance plant tolerance to abiotic stresses.…”

Section: Introductionmentioning

confidence: 65%

Appropriate Ammonium-Nitrate Ratio Improves Nutrient Accumulation and Fruit Quality in Pepper (Capsicum annuum L.)

Zhang

Dawuda

et al. 2019

Agronomy

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Ammonium (NH4+) and nitrate (NO3−) are the two forms of inorganic nitrogen essential for physiological and biochemical processes in higher plants, but little is known about how the NH4+:NO3− ratio may affect nitrogen metabolism. This study determined the effect of NH4+:NO3− ratios on plant growth, accumulation, and distribution of nutrient elements, fruit quality, enzyme activity, and relative expression of genes involved in nitrogen (N) metabolism in pepper (Capsicum annuum L.). In a pod experiment, the NH4+:NO3− ratios of 0:100, 12.5:87.5, 25:75, 37.5:62.5, and 50:50 were arranged in a complete randomized design with three replicates. The application of NH4+:NO3− at 25:75 resulted in highest dry matter and N, phosphorus (P), and potassium (K) accumulation. Pepper treated with 25:75 ratio increased root length, surface areas, and root volume and tips. The contents of vitamin C, soluble sugar, soluble protein, total phenols, flavonoids, and capsaicinoids in the fruits were significantly higher with the NH4+:NO3− ratio of 25:75 compared with 0:100 treatment, while lowering nitrate content was found in NH4+:NO3− ratios of 25:75, 37.5:62.5, and 50:50 treatments. Activity of glutamine synthetase (GS), glutamate synthases (GOGAT) enzyme and the levels of relative expression of genes coding these enzymes were superior when the NH4+:NO3− ratio of 25:75 were applied. Therefore, an appropriate ratio of NH4+:NO3− (25:75) in nitrogen application can stimulate root development, promote enzyme activities, and enhance the productivity and fruit quality in pepper.

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“…Tryptophan-derived indole-3-acetaldoxime in the indolic group—also known as a precursor for auxin related to plant growth and development—and camalexin biosynthesis controlling deter bacterial and fungal pathogens [ 34 , 35 ]. Recently, some aliphatic and indolic GSLs have been reported that are involved in primary metabolism in plants, such as nitrogen and sulfur sources, and abiotic stresses, such as salinity, light, and elevated CO 2 [ 36 , 37 , 38 , 39 , 40 ].…”

Section: Metabolic and Regulatory Pathways Of Gsl Biosynthesismentioning

confidence: 99%

Understanding of MYB Transcription Factors Involved in Glucosinolate Biosynthesis in Brassicaceae

Seo

Kim

2017

Molecules

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Glucosinolates (GSLs) are widely known secondary metabolites that have anticarcinogenic and antioxidative activities in humans and defense roles in plants of the Brassicaceae family. Some R2R3-type MYB (myeloblastosis) transcription factors (TFs) control GSL biosynthesis in Arabidopsis. However, studies on the MYB TFs involved in GSL biosynthesis in Brassica species are limited because of the complexity of the genome, which includes an increased number of paralog genes as a result of genome duplication. The recent completion of the genome sequencing of the Brassica species permits the identification of MYB TFs involved in GSL biosynthesis by comparative genome analysis with A. thaliana. In this review, we describe various findings on the regulation of GSL biosynthesis in Brassicaceae. Furthermore, we identify 63 orthologous copies corresponding to five MYB TFs from Arabidopsis, except MYB76 in Brassica species. Fifty-five MYB TFs from the Brassica species possess a conserved amino acid sequence in their R2R3 MYB DNA-binding domain, and share close evolutionary relationships. Our analysis will provide useful information on the 55 MYB TFs involved in the regulation of GSL biosynthesis in Brassica species, which have a polyploid genome.

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Quantitative proteomics reveals the importance of nitrogen source to control glucosinolate metabolism inArabidopsis thalianaandBrassica oleracea

Abstract: HighlightA quantitative proteomic approach demonstrates how ammonium nutrition induces glucosinolate biosynthetic and catabolic pathways in Arabidopsis and broccoli.

Cited by 52 publications

References 58 publications

Leaves play a central role in the adaptation of nitrogen and sulfur metabolism to ammonium nutrition in oilseed rape (Brassica napus)

Leaves play a central role in the adaptation of nitrogen and sulfur metabolism to ammonium nutrition in oilseed rape (Brassica napus)

Appropriate Ammonium-Nitrate Ratio Improves Nutrient Accumulation and Fruit Quality in Pepper (Capsicum annuum L.)

Understanding of MYB Transcription Factors Involved in Glucosinolate Biosynthesis in Brassicaceae

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