Quantitative proteomics reveals a role of JAZ7 in plant defense response to Pseudomonas syringae DC3000

Zhang, Tong; Li, Meng; Kong, Wenwen; Yin, Zepeng; Wang, Yan; Schneider, Jacqueline D.; Chen, Sixue

doi:10.1016/j.jprot.2018.01.002

Cited by 19 publications

(5 citation statements)

References 70 publications

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“…The jasmonate ZIM-domain 7 (JAZ7) protein generally functions as a transcriptional repressor regulating various biological processes, such as increasing the susceptibility of Arabidopsis to the fungal pathogen Fusarium oxysporum [103], and this protein is also involved in the defense response against Pseudomonas syringae pv. tomato DC3000 (PstDC3000) via regulation of ROS, the energy balance and glucosinolate biosynthesis [104].…”

Section: Understanding the Molecular Mechanisms Of The Plant Responsementioning

confidence: 99%

Proteomics: a powerful tool to study plant responses to biotic stress

et al. 2019

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In recent years, mass spectrometry-based proteomics has provided scientists with the tremendous capability to study plants more precisely than previously possible. Currently, proteomics has been transformed from an isolated field into a comprehensive tool for biological research that can be used to explain biological functions. Several studies have successfully used the power of proteomics as a discovery tool to uncover plant resistance mechanisms. There is growing evidence that indicates that the spatial proteome and post-translational modifications (PTMs) of proteins directly participate in the plant immune response. Therefore, understanding the subcellular localization and PTMs of proteins is crucial for a comprehensive understanding of plant responses to biotic stress. In this review, we discuss current approaches to plant proteomics that use mass spectrometry, with particular emphasis on the application of spatial proteomics and PTMs. The purpose of this paper is to investigate the current status of the field, discuss recent research challenges, and encourage the application of proteomics techniques to further research.

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Section: Understanding the Molecular Mechanisms Of The Plant Responsementioning

confidence: 99%

Proteomics: a powerful tool to study plant responses to biotic stress

et al. 2019

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“…Previous studies have shown that the changes at the protein and phosphoprotein levels are not directly regulated at the transcription level in pathogens infected tissues ( Hou et al, 2015 ; Zhang et al, 2018 ; Pang et al, 2020 ). To investigate the correlation of the mRNA transcript abundance with the protein phosphorylation intensity level during SAR, expression of significantly changed phosphoproteins at the mRNA level was evaluated by randomly selecting eight of them and determining their relative gene transcription levels by RT-qPCR.…”

Section: Resultsmentioning

confidence: 99%

Protein Phosphorylation Changes During Systemic Acquired Resistance in Arabidopsis thaliana

Zhou

Tan

et al. 2021

Front. Plant Sci.

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Systemic acquired resistance (SAR) in plants is a defense response that provides resistance against a wide range of pathogens at the whole-plant level following primary infection. Although the molecular mechanisms of SAR have been extensively studied in recent years, the role of phosphorylation that occurs in systemic leaves of SAR-induced plants is poorly understood. We used a data-independent acquisition (DIA) phosphoproteomics platform based on high-resolution mass spectrometry in an Arabidopsis thaliana model to identify phosphoproteins related to SAR establishment. A total of 8011 phosphorylation sites from 3234 proteins were identified in systemic leaves of Pseudomonas syringae pv. maculicola ES4326 (Psm ES4326) and mock locally inoculated plants. A total of 859 significantly changed phosphoproteins from 1119 significantly changed phosphopeptides were detected in systemic leaves of Psm ES4326 locally inoculated plants, including numerous transcription factors and kinases. A variety of defense response-related proteins were found to be differentially phosphorylated in systemic leaves of Psm ES4326 locally inoculated leaves, suggesting that these proteins may be functionally involved in SAR through phosphorylation or dephosphorylation. Significantly changed phosphoproteins were enriched mainly in categories related to response to abscisic acid, regulation of stomatal movement, plant–pathogen interaction, MAPK signaling pathway, purine metabolism, photosynthesis-antenna proteins, and flavonoid biosynthesis. A total of 28 proteins were regulated at both protein and phosphorylation levels during SAR. RT-qPCR analysis revealed that changes in phosphorylation levels of proteins during SAR did not result from changes in transcript abundance. This study provides comprehensive details of key phosphoproteins associated with SAR, which will facilitate further research on the molecular mechanisms of SAR.

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“…Research on abiotic stress responses in plants had been aided by the development of novel large-scale omics techniques including transcriptomics, proteomics, and metabolomics [ 38 , 39 , 40 ]. Application of these approaches would allow the discovery of new molecules and pathways that are significantly changed in transgenic plants overexpressing PAL .…”

Section: Discussionmentioning

confidence: 99%

A Protective Role of Phenylalanine Ammonia-Lyase from Astragalus membranaceus against Saline-Alkali Stress

Fan

Shi

Yang

et al. 2022

IJMS

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Phenylalanine ammonia-lyase (PAL, E.C.4.3.1.5) catalyzes the benzene propane metabolism and is the most extensively studied enzyme of the phenylpropanoid pathway. However, the role of PAL genes in Astragalus membranaceus, a non-model plant showing high capability toward abiotic stress, is less studied. Here, we cloned AmPAL and found that it encodes a protein that resides in the cytoplasmic membrane. The mRNA of AmPAL was strongly induced by NaCl or NaHCO3 treatment, especially in the root. Overexpressing AmPAL in Nicotiana tabacum resulted in higher PAL enzyme activities, lower levels of malondialdehyde (MDA), and better root elongation in the seedlings under stress treatment compared to the control plants. The protective role of AmPAL under saline-alkali stress was also observed in 30-day soil-grown plants, which showed higher levels of superoxide dismutase (SOD), proline, and chlorophyll compared to wild-type N. Tabacum. Collectively, we provide evidence that AmPAL is responsive to multiple abiotic stresses and that manipulating the expression of AmPAL can be used to increase the tolerance to adverse environmental factors in plants.

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Quantitative proteomics reveals a role of JAZ7 in plant defense response to Pseudomonas syringae DC3000

Cited by 19 publications

References 70 publications

Proteomics: a powerful tool to study plant responses to biotic stress

Proteomics: a powerful tool to study plant responses to biotic stress

Protein Phosphorylation Changes During Systemic Acquired Resistance in Arabidopsis thaliana

A Protective Role of Phenylalanine Ammonia-Lyase from Astragalus membranaceus against Saline-Alkali Stress

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