Quantitative proteomics analysis reveals the response mechanism of peanut (Arachis hypogaea L.) to imbibitional chilling stress

Abstract

Imbibitional chilling stress inhibits normal seed germination and seedling establishment and leads to large losses in peanut production. This is a major limiting factor when sowing peanut earlier and further north. To reveal the response mechanism of peanut to imbibitional chilling stress, a Tandem Mass Tag (TMT)‐based quantitative proteomics analysis was conducted to identify differentially accumulated proteins (DAPs) under imbibitional chilling stress. Hormone profiling and transcriptional analysis were performed to confirm the proteomics data. Further seed priming analysis with exogenous cytokinins was conducted to validate the role of cytokinins in alleviating imbibitional chilling injury. A total of 5029 proteins were identified and quantified in all of the experimental groups. Among these, 104 proteins were DAPs as compared with the control. Enrichment analysis revealed that these DAPs were significant in various molecular functional and biological processes, especially for biosynthesis and metabolism of plant hormones. Hormone profiling and transcription analysis suggested that the reduced abundance of cytokinin oxidase may be caused by down‐regulation of gene expression of the corresponding genes and leads to an elevated content of cytokinins under chilling stress. Seed priming analysis suggested that exogenous application of cytokinins may alleviate injury caused by imbibitional chilling. Our study provides a comprehensive proteomics analysis of peanut under imbibitional chilling stress, suggesting the role of plant hormones in the response mechanism. The results provide a better understanding of the imbibitional chilling stress response mechanism in peanut that will aid in peanut production.

Antioxidant Regulation and DNA Methylation Dynamics During Mikania micrantha Seed Germination Under Cold Stress

“…Oxidative stress changes the lipid composition of the cell membrane, resulting in reduced permeability and fluidity. This phenomenon has been reported in many crop plants, including maize, cotton, and peanut (He et al, 2016;Gao et al, 2020;Chen et al, 2021). Moreover, the overaccumulation of H 2 O 2 and O 2 − leads to oxidative stress, which can strongly promote non-enzymatic lipid peroxidation, in which MDA is an important end product (Hodges et al, 1999;Sattler et al, 2006).…”

Transcriptomic–Proteomic Analysis Revealed the Regulatory Mechanism of Peanut in Response to Fusarium oxysporum

“…In peanut research, omics analysis has been widely applied in various aspects. For instance, in investigating the response mechanism of peanuts to low-temperature stress during imbibition, 5029 proteins were identified and quantified; among them, 104 proteins were found to be differentially expressed [ 37 ]. However, there are no reports on combined omics analysis elucidating the mechanisms involved in the interaction between peanuts and Fusarium.…”

Optimizing sowing date and plant density improve peanut yield by mitigating heat and chilling stress

“…Heat and chilling are two major climatic stresses that adversely affect peanut growth (Song et al., 2020), downward pressure on productivity can be expected to be further exacerbated by climate change (Foyer et al., 2016; Faye et al., 2018; Tian et al., 2019). The growth of peanuts will be severely inhibited when the air temperature is below 20°C, and chilling stress (especially in flowering stages) is deemed as a limiting factor in peanut production (Bell et al., 1994; Chen et al., 2021; Prasad et al., 2006). Reduced temperature decreases CO 2 assimilation through decreased enzyme activity, limiting photosynthesis and associated chemical energy production (Li et al., 2004; Yamori, 2016; Yu et al., 2002), resulting in severely immature pods development (Chakraborty et al., 2018; Fotiadis et al., 2017).…”