Quantitative proteomics analysis reveals glutamine deprivation activates fatty acid β-oxidation pathway in HepG2 cells

Long, Baisheng; Rodiallah, Muhammad; Yan, Guokai; Yu, Jie; Fan, Qi-Wen; Wang, Zhi‐Chang; Li, Xiuzhi; Purnomoadi, Agung; Achmadi, Joelal; Yan, Xianghua

doi:10.1007/s00726-016-2182-7

Cited by 11 publications

(9 citation statements)

References 49 publications

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“…The normalization of weight was regulated by increased energy expenditure, but not by caloric restriction or increased activity, and a transient induction of the energy balance regulating hormone fibroblast growth factor 21 (FGF21) play a critical role in this procession [ 30 ]. Glutamine as Leu metabolites plays a crucial role in various cellular processes, such as energy balance, apoptosis, and cell proliferation; it could activate the fatty acid β-oxidation pathway in HepG2 cells [ 31 ]. This result shows that glutamine deprivation can activate the fatty acid β-oxidation pathway to regulate lipid metabolism.…”

Section: Roles Of Bcaas In Nutrition Metabolismmentioning

confidence: 99%

Branched Chain Amino Acids: Beyond Nutrition Metabolism

Nie

Zhang

et al. 2018

IJMS

456

324

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Branched chain amino acids (BCAAs), including leucine (Leu), isoleucine (Ile), and valine (Val), play critical roles in the regulation of energy homeostasis, nutrition metabolism, gut health, immunity and disease in humans and animals. As the most abundant of essential amino acids (EAAs), BCAAs are not only the substrates for synthesis of nitrogenous compounds, they also serve as signaling molecules regulating metabolism of glucose, lipid, and protein synthesis, intestinal health, and immunity via special signaling network, especially phosphoinositide 3-kinase/protein kinase B/mammalian target of rapamycin (PI3K/AKT/mTOR) signal pathway. Current evidence supports BCAAs and their derivatives as the potential biomarkers of diseases such as insulin resistance (IR), type 2 diabetes mellitus (T2DM), cancer, and cardiovascular diseases (CVDs). These diseases are closely associated with catabolism and balance of BCAAs. Hence, optimizing dietary BCAA levels should have a positive effect on the parameters associated with health and diseases. This review focuses on recent findings of BCAAs in metabolic pathways and regulation, and underlying the relationship of BCAAs to related disease processes.

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Section: Roles Of Bcaas In Nutrition Metabolismmentioning

confidence: 99%

Branched Chain Amino Acids: Beyond Nutrition Metabolism

Nie

Zhang

et al. 2018

IJMS

456

324

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“…Glutamine plays a crucial role in various cellular processes, such as in energy balance, apoptosis, and cell proliferation and, its deprivation can activate the fatty acid β-oxidation pathway [150,151]. For instance, there has been a controversy linking glutamine with the prediction of the T2D.…”

Section: Glutamate Family: Glutamine and Glutamatementioning

confidence: 99%

Detection of Early Disease Risk Factors Associated with Metabolic Syndrome: A New Era with the NMR Metabolomics Assessment

et al. 2020

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The metabolic syndrome is a multifactorial disease developed due to accumulation and chronification of several risk factors associated with disrupted metabolism. The early detection of the biomarkers by NMR spectroscopy could be helpful to prevent multifactorial diseases. The exposure of each risk factor can be detected by traditional molecular markers but the current biomarkers have not been enough precise to detect the primary stages of disease. Thus, there is a need to obtain novel molecular markers of pre-disease stages. A promising source of new molecular markers are metabolomics standing out the research of biomarkers in NMR approaches. An increasing number of nutritionists integrate metabolomics into their study design, making nutrimetabolomics one of the most promising avenues for improving personalized nutrition. This review highlight the major five risk factors associated with metabolic syndrome and related diseases including carbohydrate dysfunction, dyslipidemia, oxidative stress, inflammation, and gut microbiota dysbiosis. Together, it is proposed a profile of metabolites of each risk factor obtained from NMR approaches to target them using personalized nutrition, which will improve the quality of life for these patients.

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“…Previously, using the iTRAQ-based approach, we have characterized the proteome of HepG2 cells treated with either Gln deprivation or Arg supplementation 19, 20 . However, to our knowledge, there is no literature reporting proteome scale alterations induced by Leu deprivation in liver cells.…”

Section: Introductionmentioning

confidence: 99%

The Fatty Acid β-Oxidation Pathway is Activated by Leucine Deprivation in HepG2 Cells: A Comparative Proteomics Study

Yan

Peng

et al. 2017

Sci Rep

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Leucine (Leu) is a multifunctional essential amino acid that plays crucial role in various cellular processes. However, the integral effect of Leu on the hepatic proteome remains largely unknown. Here, we for the first time applied an isobaric tags for relative and absolute quantification (iTRAQ)-based comparative proteomics strategy to investigate the proteome alteration induced by Leu deprivation in human hepatocellular carcinoma (HepG2) cells. A total of 4,111 proteins were quantified; 43 proteins were further identified as differentially expressed proteins between the normal and Leu deprivation groups. Bioinformatics analysis showed that the differentially expressed proteins were involved in various metabolic processes, including amino acid and lipid metabolism, as well as degradation of ethanol. Interestingly, several proteins involved in the fatty acid β-oxidation pathway, including ACSL1, ACADS, and ACOX1, were up-regulated by Leu deprivation. In addition, Leu deprivation led to the reduction of cellular triglycerides in HepG2 cells. These results reveal that the fatty acid β-oxidation pathway is activated by Leu deprivation in HepG2 cells, and provide new insights into the regulatory function of Leu in multiple cellular processes, especially fatty acid metabolism.

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Quantitative proteomics analysis reveals glutamine deprivation activates fatty acid β-oxidation pathway in HepG2 cells

Cited by 11 publications

References 49 publications

Branched Chain Amino Acids: Beyond Nutrition Metabolism

Branched Chain Amino Acids: Beyond Nutrition Metabolism

Detection of Early Disease Risk Factors Associated with Metabolic Syndrome: A New Era with the NMR Metabolomics Assessment

The Fatty Acid β-Oxidation Pathway is Activated by Leucine Deprivation in HepG2 Cells: A Comparative Proteomics Study

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