Quantitative Proteomics Analysis of Streptomyces coelicolor Development Demonstrates That Onset of Secondary Metabolism Coincides with Hypha Differentiation

Abstract

Streptomyces species produce many clinically important secondary metabolites, including antibiotics and antitumorals. They have a complex developmental cycle, including programmed cell death phenomena, that makes this bacterium a multicellular prokaryotic model. There are two differentiated mycelial stages: an early compartmentalized vegetative mycelium (first mycelium) and a multinucleated reproductive mycelium (second mycelium) arising after programmed cell death processes. In the present study, we made a detailed proteomics analysis of the distinct developmental stages of solid confluent Streptomyces coelicolor cultures using iTRAQ (isobaric tags for relative and absolute quantitation) labeling and LC-MS/MS. A new experimental approach was developed to obtain homogeneous samples at each developmental stage (temporal protein analysis) and also to obtain membrane and cytosolic protein fractions (spatial protein analysis). A total of 345 proteins were quantified in two biological replicates. Comparative bioinformatics analyses revealed the switch from primary to secondary metabolism between the initial compartmentalized mycelium and the multinucleated hyphae. Molecular & Cellular Proteomics 9:1423-1436, 2010.

Quantitative Proteome Analysis of Streptomyces coelicolor Nonsporulating Liquid Cultures Demonstrates a Complex Differentiation Process Comparable to That Occurring in Sporulating Solid Cultures

“…27,28 Recently, we reported the first quantitative mass spectrometrydriven proteome analysis of Streptomyces differentiation in solid cultures, 17 demonstrating the switch from primary to secondary metabolism in MI and MII.…”

Saccharopolyspora erythraea’sgenome is organised in high-order transcriptional regions mediated by targeted degradation at the metabolic switch

“…S.erythraea exhibits a distinct and complex lifecycle, in which an initial growth phase is followed by a transition period, known as the metabolic switch, followed by a secondary growth phase. Like in Streptomyces coelicolor ( S.coelicolor ), the switch in S.erythraea is followed by morphological changes that coincide with potential cell death and the transcription of secondary metabolite gene clusters [4]. A detailed study of transcriptional activity during this transitional period is of major importance to understand the complex, yet little understood, life cycle of this sophisticated bacterial phylum.…”

Multiple-platform data integration method with application to combined analysis of microarray and proteomic data

“…These genes are up-regulated only at late stages of the culture and produce antibiotics during the stationary phase. Expression of two genes encoding malate oxidoreductase (SCO2951) and translation elongation factor G (SCO4661) have been found to be depressed during the stationary phase compared with the growth phase [17]. Table 5 summarizes the nine genes and the associated literature confirmations [16-21].…”