Quantitative proteomic sequencing of F
 1 hybrid populations reveals the function of sorbitol in apple resistance to Botryosphaeria dothidea

He, Xin-Qiang; Meng, Hui; Wang, Haibo; He, Ping; Chang, Yuansheng; Wang, Sen; Wang, Chuan‐Zeng; Li, Linguang; Wang, Chen

doi:10.1093/hr/uhac115

Cited by 7 publications

(5 citation statements)

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“…Previous studies have reported that overexpression of MdFLS2 in Arabidopsis enhances salicylic acid signaling and improves resistance to Botryosphaeria dothidea in Arabidopsis ( Liu et al., 2018 ). There are some similarities in the disease resistance mechanisms of apples to AAAP and Botryosphaeria dothidea ; for example, sorbitol can enhance the resistance of apples to both AAAP and Botryosphaeria dothidea ( Meng et al., 2018 ; He et al., 2022 ).…”

Section: Resultsmentioning

confidence: 99%

Insertion of a TRIM-like sequence in MdFLS2-1 promoter is associated with its allele-specific expression in response to Alternaria alternata in apple

Liang

Jiang

et al. 2022

Front. Plant Sci.

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Alternaria blotch disease, caused by Alternaria alternata apple pathotype (AAAP), is one of the major fungal diseases in apple. Early field observations revealed, the anther-derived homozygote Hanfu line (HFTH1) was highly susceptible to AAAP, whereas Hanfu (HF) exhibited resistance to AAAP. To understand the molecular mechanisms underlying the difference in sensitivity of HF and HFTH1 to AAAP, we performed allele-specific expression (ASE) analysis and comparative transcriptomic analysis before and after AAAP inoculation. We reported an important immune gene, namely, MdFLS2, which displayed strong ASE in HF with much lower expression levels of HFTH1-derived alleles. Transient overexpression of the dominant allele of MdFLS2-1 from HF in GL-3 apple leaves could enhance resistance to AAAP and induce expression of genes related to salicylic acid pathway. In addition, MdFLS2-1 was identified with an insertion of an 85-bp terminal-repeat retrotransposon in miniature (TRIM) element-like sequence in the upstream region of the nonreference allele. In contrast, only one terminal direct repeat (TDR) from TRIM-like sequence was present in the upstream region of the HFTH1-derived allele MdFLS2-2. Furthermore, the results of luciferase and β-glucuronidase reporter assays demonstrated that the intact TRIM-like sequence has enhancer activity. This suggested that insertion of the TRIM-like sequence regulates the expression level of the allele of MdFLS2, in turn, affecting the sensitivity of HF and HFTH1 to AAAP.

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Section: Resultsmentioning

confidence: 99%

Insertion of a TRIM-like sequence in MdFLS2-1 promoter is associated with its allele-specific expression in response to Alternaria alternata in apple

Liang

Jiang

et al. 2022

Front. Plant Sci.

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“…The recombined constructs were transformed into Agrobacterium tumefaciens LBA4404. The transgenic apple calli were obtained as described by He et al [18,44]. MdSYP121-TRV2 for VIGS was obtained by cloning selected fragments from MdSYP121 into a pTRV2 vector.…”

Section: Gene Cloning Vector Construction and Virus-induced Gene Sile...mentioning

confidence: 99%

“…RT-qPCR was performed on the CFX96TM Real-time Detection System. The operational procedure was as described by He et al [44]. The Malus × domestica actin gene (MdActin, GenBank ID XM029089583.1) was used as the housekeeping control.…”

Section: Rna Extraction and Rt-qpcr Assaysmentioning

confidence: 99%

Metabolome and Transcriptome Profiling Reveals the Function of MdSYP121 in the Apple Response to Botryosphaeria dothidea

Zhang,

Wang,

Wang

et al. 2023

IJMS

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The vesicular transport system is important for substance transport in plants. In recent years, the regulatory relationship between the vesicular transport system and plant disease resistance has received widespread attention; however, the underlying mechanism remains unclear. MdSYP121 is a key protein in the vesicular transport system. The overexpression of MdSYP121 decreased the B. dothidea resistance of apple, while silencing MdSYP121 resulted in the opposite phenotype. A metabolome and transcriptome dataset analysis showed that MdSYP121 regulated apple disease resistance by significantly affecting sugar metabolism. HPLC results showed that the levels of many soluble sugars were significantly higher in the MdSYP121-OE calli. Furthermore, the expression levels of genes related to sugar transport were significantly higher in the MdSYP121-OE calli after B. dothidea inoculation. In addition, the relationships between the MdSYP121 expression level, the soluble sugar content, and apple resistance to B. dothidea were verified in an F1 population derived from a cross between ‘Golden Delicious’ and ‘Fuji Nagafu No. 2’. In conclusion, these results suggested that MdSYP121 negatively regulated apple resistance to B. dothidea by influencing the soluble sugar content. These technologies and methods allow us to investigate the molecular mechanism of the vesicular transport system regulating apple resistance to B. dothidea.

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“…SOX catalyzed the irreversible reaction of sorbitol and glucose, whereas NADP‐sorbitol dehydrogenase (NADP‐SDH) and NAD‐sorbitol dehydrogenase (NAD‐SDH) catalyzed the reciprocal conversion between sorbitol and glucose, and the reversible oxidation of sorbitol to fructose, respectively 19 . Further research revealed that sorbitol played major roles in disease resistance to Botryosphaeria dothidea and A. alternata of apples 21,22 . Study has shown that spraying with zinc sulfate enhanced SDH activity which contributes to the accumulation of carbohydrate in apples 23 .…”

Section: Introductionmentioning

confidence: 99%

“…19 Further research revealed that sorbitol played major roles in disease resistance to Botryosphaeria dothidea and A. alternata of apples. 21,22 Study has shown that spraying with zinc sulfate enhanced SDH activity which contributes to the accumulation of carbohydrate in apples. 23 A study in apricots showed that oxalic acid enhanced NAD-SDH and SOX activities, whereas decreased the level of sorbitol.…”

Section: Introductionmentioning

confidence: 99%

l‐Glutamate maintains the quality of apple fruit by mediating carotenoid, sorbitol and sucrose metabolisms

Zhang

Liu

et al. 2023

J Sci Food Agric

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Background: L-Glutamate is involved in many important chemical reactions in horticultural products and improves postharvest disease resistance. Quality decline of apple fruit caused by senescence and fungus invasion often leads to tremendous losses during logistics. This study was performed to evaluate the variations of quality attributes, carotenoid, sorbitol and sucrose metabolisms in apples (cv. Qiujin) after L-glutamate dipping treatment. RESUITS: L-Glutamate immersion maintained high values of L*, a* and b*, flesh firmness, titratable acidity, as well as the total soluble solids, soluble sugar, reducing sugar and ascorbic acid contents in apples. L-Glutamate also decreased mass loss, respiratory rate and ethylene release, enhanced sucrose synthase-cleavage, acid invertase and neutral invertase activities, whereas reduced sorbitol dehydrogenase, sucrose phosphate synthase, sucrose synthase synthesis and sorbitol oxidase activities in apples. Moreover, L-glutamate inhibited lutein, ⊎-carotene and lycopene accumulation, and downregulated phytoene synthase, lycopene ⊎-cyclase, ζ-carotene desaturase, phytoene desaturase, carotenoid isomerase, ζ-carotene isomerase and carotenoids cleavage dioxygenase gene expressions, but up-regulated 9-cis-epoxycarotenoid dioxygenase gene expression in apples.Conclusion: Postharvest L-glutamate dipping treatment can keep apple quality by modulating key enzyme activity and gene expression in sorbitol, sucrose and carotenoid metabolisms.

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Quantitative proteomic sequencing of F 1 hybrid populations reveals the function of sorbitol in apple resistance to Botryosphaeria dothidea

Cited by 7 publications

References 50 publications

Insertion of a TRIM-like sequence in MdFLS2-1 promoter is associated with its allele-specific expression in response to Alternaria alternata in apple

Insertion of a TRIM-like sequence in MdFLS2-1 promoter is associated with its allele-specific expression in response to Alternaria alternata in apple

Metabolome and Transcriptome Profiling Reveals the Function of MdSYP121 in the Apple Response to Botryosphaeria dothidea

l‐Glutamate maintains the quality of apple fruit by mediating carotenoid, sorbitol and sucrose metabolisms

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