Quantitative proteomic Analysis Reveals up-regulation of caveolin-1 in FOXP3-overexpressed human gastric cancer cells

Abstract

Forkhead box protein 3 (FOXP3) is implicated in tumor progression and prognosis in various types of tumor cells. We have recently reported that FOXP3 inhibited proliferation of gastric cancer (GC) cells through activating the apoptotic signaling pathway. In this study, we found that over-expression of FOXP3 inhibited GC cell migration, invasion and proliferation. Then, the label-free quantitative proteomic approach was employed to further investigating the down-stream proteins regulated by FOXP3, resulting in a total of 3,978 proteins quantified, including 186 significantly changed proteins. Caveolin-1 (CAV1), as a main constituent protein of caveolae, was one of those changed proteins up-regulated in FOXP3-overexpressed GC cells, moreover, it was assigned as one of the node proteins in the protein-protein interaction network and the key protein involved in focal adhesion pathway by bioinformatics analysis. Further biological experiments confirmed that FOXP3 directly bound to the promoter regions of CAV1 to positively regulate CAV1 transcription in GC cells. In summary, our study suggested that FOXP3 can be considered as a tumor suppressor in GC via positively regulating CAV1 through transcriptional activation, and this FOXP3-CAV1 transcriptional regulation axis may play an important role in inhibiting invasion and metastasis of GC cells. Data are available via ProteomeXchange under identifier PXD007725.

miR‐133a‐3p/FOXP3 axis regulates cell proliferation and autophagy in gastric cancer

“…Interestingly, FOXP3 seems to have the opposite effect on cancer progression in different cancers . Our previous and other studies have found that FOXP3 is closely related to the development of GC, and its specific role and expression of FOXP3 in the progression of GC . The specific and in‐depth study of the exact mechanism of regulation may provide new ideas and theoretical basis for the clinical treatment of GC and the development of new drug targets.…”

The Role of FOXP3 on Tumor Metastasis and Its Interaction with Traditional Chinese Medicine

“…A study of the molecular mechanism showed that FOXP3 promotes tumor metastasis and invasion might be associated with the upregulation of MMP2 and MMP9 expression. Although this study also found that patients with low levels of FOXP3 had slightly higher median survival, there was no statistically significant difference [ 19 ] ( Table 2 ).…”

In vitro toxicity assessment of emitted materials collected during the manufacture of water pipe plastic linings

“…Fold changes from groups were then averaged and statistically compared using t-tests (p < 0.05). This statistical approach has been utilized in previous evaluations employing untargeted proteomic approaches [41,42,43,44]. Specifically, Pascovici et al demonstrated that t-tests are appropriate as opposed to multiple-comparison corrections due to a number of statistically limiting factors (few replicates, ratio compression, effect size, and other constraints) related to exploratory proteomic studies resulting in the exclusion of all true positives [45].…”