Quantitative Proteomic Analysis Reveals Synaptic Dysfunction in the Amygdala of Rats Susceptible to Chronic Mild Stress

Zhou, Mei; Liu, Zhao; Yu, Jia; Li, Shuiming; Tang, Min; Zeng, Li; Wang, Haiyang; Xie, Hui; Peng, Li; Huang, Haojun; Zhou, Chanjuan; Xie, Peng; Zhou, Jian

doi:10.1016/j.neuroscience.2018.02.010

Cited by 23 publications

(25 citation statements)

References 83 publications

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“…2014; Zhou et al, 2018). Gad1, Gad2 and Glul, are the key regulatory enzymes that maintain the metabolic homeostasis of GABA and glutamate in the glutamate-GABA-glutamine cycle between neurons and astrocytes (Veeraiah et al, 2014;Wang et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Perturbation of Ephrin Receptor Signaling and Glutamatergic Transmission in the Hypothalamus in Depression Using Proteomics Integrated With Metabolomics

Wei

et al. 2019

Front. Neurosci.

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Section: Discussionmentioning

confidence: 99%

Perturbation of Ephrin Receptor Signaling and Glutamatergic Transmission in the Hypothalamus in Depression Using Proteomics Integrated With Metabolomics

Wei

et al. 2019

Front. Neurosci.

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“…Protein identification and iTRAQ quantitation were conducted by AB SCIEX ProteinPilot software 4.5 with the Paragon database search algorithm (4.5.0.0, 1654; Zhou et al ). The search engine included false discovery rate (FDR) analysis.…”

Section: Methodsmentioning

confidence: 99%

“…Search criteria were trypsin specificity, fixed modification of carbamidomethyl, variable modifications of oxidation and phosphorylation and two allowed missed cleavages. Database search parameters were used as described previously with some modifications (Zhou et al ). For this study, peptides were filtered at 90% confidence, and the proteins were grouped using the AB SCIEX ProGroup algorithm for redundancy minimization.…”

Section: Methodsmentioning

confidence: 99%

Quantitative phosphoproteomics of lectin receptor‐like kinase VI.4 dependent abscisic acid response in Arabidopsis thaliana

Zhang

Guo

Xie

et al. 2018

Physiologia Plantarum

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Lectin receptor-like kinases (LecRKs) play important roles in the responses to adverse environment stress. Abscisic acid (ABA) is a plant hormone involved in plant growth, development and adverse environmental stress responses. Although some studies of ABA response LecRK genes have been reported, the molecular mechanisms of LecRKs regulation of downstream pathways under ABA induction are not well understood. The present study showed that LecRK-VI.4 responded to ABA and negatively regulated stomatal closure. Here, a quantitative phosphoproteomics approach based on mass spectrometry was employed to study the roles of LecRK-VI.4 in the ABA signaling pathway. Metal oxide affinity beads and C18 chromatography were used for phosphopeptide enrichment and separation. The isobaric tags for relative and absolute quantitation were used for profiling the phosphoproteome of mutant lecrk-vi.4-1 and wild-type Col-0 Arabidopsis under normal growth conditions or ABA treatments. In total, 475 unique phosphopeptides were quantified, including 81 phosphopeptides related to LecRK-VI.4 regulation. Gene ontology, protein-protein interaction and motif analysis were performed. The bioinformatics data showed that phosphorylated proteins regulated by LecRK-VI.4 had close relations with factors of stomatal function, which included aquaporin activity, H pump activity and the Ca concentration in the cytoplasm. These data have expanded our understanding of how LecRK-VI.4 regulates ABA-mediated stomatal movements.

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“…Such studies have highlighted the involvement of synaptic glutamate signaling in mediating plasticity of the amygdala after 8 weeks of chronic stress and identified networks of genes that are regulated at various timepoints after chronic social defeat, two paradigms that induce symptoms of major depressive disorder in rodents [28]. Similarly, we previously employed a stress-enhanced fear learning paradigm that combines a single acute restraint stress session with Pavlovian fear conditioning and used RNA-sequencing to identify differential expression of networks of genes involved in learning and plasticity in the BLC 30 days after the initial stress event [30].…”

Section: Introductionmentioning

confidence: 99%

Bioinformatic analysis of long-lasting transcriptional and translational changes in the basolateral amygdala following acute stress

Sillivan

Jones

Jamieson

et al. 2018

Preprint

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Stress profoundly impacts the brain and increases the risk of developing a psychiatric disorder. The brain's response to stress is mediated by a number of pathways that affect gene expression and protein function throughout the cell. Understanding how stress achieves such dramatic effects on the brain requires an understanding of the brain's stress response pathways. The majority of studies focused on molecular changes have employed repeated or chronic stress paradigms to assess the long-term consequences of stress and have not taken an integrative genomic and/or proteomic approach. Here, we determined the lasting impact of a single stressful event (restraint) on the broad molecular profile of the basolateral amygdala complex (BLC), a key brain region mediating emotion, memory and stress. Molecular profiling performed thirty days post-restraint consisted of small RNA sequencing, RNA sequencing and quantitative mass spectrometry and identified long-lasting changes in microRNA (miRNA), messenger RNA (mRNA) and proteins. Alignment of the three datasets further delineated the regulation of stress-specific pathways which were validated by qPCR and Western Blot analysis. From this analysis, mir-29a-5p was identified as a putative regulator of stress-induced adaptations in the BLC. Further, a number of predicted mir-29a-5p targets are regulated at the mRNA and protein level. The concerted and long-lasting disruption of multiple molecular pathways in the amygdala by a single stress event is expected to be sufficient to alter behavioral responses to a wide array of future experiences, including exposure to additional stressors.

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Quantitative Proteomic Analysis Reveals Synaptic Dysfunction in the Amygdala of Rats Susceptible to Chronic Mild Stress

Cited by 23 publications

References 83 publications

Perturbation of Ephrin Receptor Signaling and Glutamatergic Transmission in the Hypothalamus in Depression Using Proteomics Integrated With Metabolomics

Perturbation of Ephrin Receptor Signaling and Glutamatergic Transmission in the Hypothalamus in Depression Using Proteomics Integrated With Metabolomics

Quantitative phosphoproteomics of lectin receptor‐like kinase VI.4 dependent abscisic acid response in Arabidopsis thaliana

Bioinformatic analysis of long-lasting transcriptional and translational changes in the basolateral amygdala following acute stress

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