Quantitative proteomic analysis reveals novel stress-associated active proteins (SAAPs) and pathways involved in modulating tolerance of wheat under terminal heat

Kumar, Ranjeet; Singh, Khushboo; Ahuja, Sanjeev; Tasleem, Mohd; Singh, Indra; Kumar, Sanjeev; Grover, Monendra; Mishra, Dwijesh Chandra; Kumar, Girjesh; Goswami, Suneha; Singh, Gyanendra; Chinnusamy, Viswanathan; Rai, Anil; Praveen, Shelly

doi:10.1007/s10142-018-0648-2

Cited by 35 publications

(24 citation statements)

References 49 publications

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“…Proteomics is the analysis of candidate proteins, the expression when they translated from mRNA to functional proteins, and a further characterization of their role in the heat tolerance mechanism [ 240 ]. Proteomic analysis revealed heat shock proteins, protein synthesis, detoxification, photosynthesis, and protein quality under heat stress conditions [ 115 , 241 , 242 , 243 , 244 , 245 ]. Hence, the omics technology provides us with a novel opportunity for the identification of genes, their expression, and pathways linked to these genes.…”

Section: Tolerance Strategies Against High Temperaturementioning

confidence: 99%

Rising Atmospheric Temperature Impact on Wheat and Thermotolerance Strategies

Khan

Ahmad

Ahmed

et al. 2020

Plants

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Temperature across the globe is increasing continuously at the rate of 0.15–0.17 °C per decade since the industrial revolution. It is influencing agricultural crop productivity. Therefore, thermotolerance strategies are needed to have sustainability in crop yield under higher temperature. However, improving thermotolerance in the crop is a challenging task for crop scientists. Therefore, this review work was conducted with the aim of providing information on the wheat response in three research areas, i.e., physiology, breeding, and advances in genetics, which could assist the researchers in improving thermotolerance. The optimum temperature for wheat growth at the heading, anthesis, and grain filling duration is 16 ± 2.3 °C, 23 ± 1.75 °C, and 26 ± 1.53 °C, respectively. The high temperature adversely influences the crop phenology, growth, and development. The pre-anthesis high temperature retards the pollen viability, seed formation, and embryo development. The post-anthesis high temperature declines the starch granules accumulation, stem reserve carbohydrates, and translocation of photosynthates into grains. A high temperature above 40 °C inhibits the photosynthesis by damaging the photosystem-II, electron transport chain, and photosystem-I. Our review work highlighted that genotypes which can maintain a higher accumulation of proline, glycine betaine, expression of heat shock proteins, stay green and antioxidant enzymes activity viz., catalase, peroxidase, super oxide dismutase, and glutathione reductase can tolerate high temperature efficiently through sustaining cellular physiology. Similarly, the pre-anthesis acclimation with heat treatment, inorganic fertilizer such as nitrogen, potassium nitrate and potassium chloride, mulches with rice husk, early sowing, presoaking of a 6.6 mM solution of thiourea, foliar application of 50 ppm dithiothreitol, 10 mg per kg of silicon at heading and zinc ameliorate the crop against the high temperature. Finally, it has been suggested that modern genomics and omics techniques should be used to develop thermotolerance in wheat.

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Section: Tolerance Strategies Against High Temperaturementioning

confidence: 99%

Rising Atmospheric Temperature Impact on Wheat and Thermotolerance Strategies

Khan

Ahmad

Ahmed

et al. 2020

Plants

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“…Wheat is ancient and staple cereal crops grown in large part of the world under a wide range of agro-climatic regions. Major share of carbohydrate and calories in the diet are provided by wheat [ 1 , 2 ]. It contains 1.5–2% fat, 1.5–2% minerals, vitamins, and 2.2% crude fibers.…”

Section: Introductionmentioning

confidence: 99%

Characterizing the putative mitogen-activated protein kinase (MAPK) and their protective role in oxidative stress tolerance and carbon assimilation in wheat under terminal heat stress

Kumar

Dubey

Arora

et al. 2021

Biotechnology Reports

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Highlights We identified and cloned heat-responsive MAPK and MAPK-1 from wheat under HS. Isolated recombinant MAPK protein of ∼40.3 kDa with high kinase activity under HS. Native wheat MAPK showed maximum activity in thermotolerant cv . under HS. MAPK showed positive correlation with tolerance, carbon flux and amylolytic linked traits. MAPK was observed to stabilize the starch quality of the grains under HS.

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“…Analysis and identi cation methods of differential proteomics in leaves of R. soongorica under stress. differential analysis and protein identi cation conducted for leaves of R. soongorica followed the described methods of Kumaravel et al 55 , Holáet et al 56 , and Kumar et al 57 , with some modi cations introduced. The preparation of proteome libraries and their deep sequencing were both performed by the Naomi Metabolic Technology Corporation (Suzhou, China).…”

Section: Determination Of Leaf Relative Conductivity and Proline Contentmentioning

confidence: 99%

Physiological Response and Proteomics Analysis of Reaumuria soongorica Under Salt Stress

Yan¹,

Chong²,

Zhao³

et al. 2021

Preprint

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Soil salinity can severely restrict plant growth. Yet Reaumuria soongorica can tolerate salinity well. However, large-scale proteomic studies of this plant’s salinity response have yet to reported. Here, R. soongorica seedlings (4 months old) were used in an experiment where NaCl solutions simulated levels of soil salinity stress. The fresh weight, root/shoot ratio, leaf relative conductivity, proline content, and total leaf area of R. soongorica under CK (0 mM NaCl), low (200 mM NaCl), and high (500 mM NaCl) salt stress were determined. The results showed that the proline content of leaves was negatively correlated with salt concentration. With greater salinity, the plant fresh weight, root/shoot ratio, and total leaf area increased initially but then decreased, and vice-versa for the relative electrical conductivity of leaves. Using iTRAQ proteomic sequencing, 47, 177, 136 differentially expressed proteins (DEPs) were identified in low-salt vs. CK, high-salt vs. control, and high-salt vs. low-salt comparisons, respectively. A total of 72 DEPs were further screened from the groups, of which, 34 DEPs increased and 38 DEPs decreased in abundance. These DEPs are mainly involved in translation, ribosomal structure, and biogenesis. Finally, 21 key DEPs (SCORE value ≥ 60 point) were identified as potential targets for salt tolerance of R. soongolica. By comparing the protein structure of treated vs. CK leaves under salt stress, we revealed the key candidate genes underpinning R. soongolica’s salt tolerance ability. This works provides fresh insight into its physiological adaptation strategy and molecular regulatory network, and a molecular basis enhancing breeding, under salt stress conditions.

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Quantitative proteomic analysis reveals novel stress-associated active proteins (SAAPs) and pathways involved in modulating tolerance of wheat under terminal heat

Cited by 35 publications

References 49 publications

Rising Atmospheric Temperature Impact on Wheat and Thermotolerance Strategies

Rising Atmospheric Temperature Impact on Wheat and Thermotolerance Strategies

Characterizing the putative mitogen-activated protein kinase (MAPK) and their protective role in oxidative stress tolerance and carbon assimilation in wheat under terminal heat stress

Physiological Response and Proteomics Analysis of Reaumuria soongorica Under Salt Stress

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