Quantitative proteomic analysis of intracerebral hemorrhage in rats with a focus on brain energy metabolism

Liu, Tao; Zhou, Jing; Cui, Hanjin; Li, Pengfei; Li, Haigang; Wang, Yang; Tang, Tao

doi:10.1002/brb3.1130

Cited by 18 publications

(15 citation statements)

References 82 publications

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“…Although there are no previous studies on the proteome of human brain following ICH, a study in a pig model of ICH showed differences in proteomic expression of whole tissue at 24 h and at 60 days compared to controls 38 . Another study in a rat model of acute ICH showed differential protein expression 39 , as did a tissue proteomic study of collagenase-induced ICH in rats at 2 days post-ICH 40 . These animal studies used whole tissue samples, and although interesting they present significant methodological differences to our present study which uses microdialysis for sampling, thus making direct comparisons difficult.…”

Section: Discussionmentioning

confidence: 94%

Dynamic protein changes in the perihaemorrhagic zone of Surgically Treated Intracerebral Haemorrhage Patients

Tobieson

Ghafouri

Zsigmond

et al. 2019

Sci Rep

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The secondary injury cascades exacerbating the initial brain injury following intracerebral haemorrhage (ICH) are incompletely understood. We used dual microdialysis (MD) catheters placed in the perihaemorrhagic zone (PHZ) and in seemingly normal cortex (SNX) at time of surgical ICH evacuation in ten patients (range 26–70 years). Routine interstitial MD markers (including glucose and the lactate/pyruvate ratio) were analysed and remaining microdialysate was analysed by two-dimensional gel electrophoresis (2-DE) and nano-liquid chromatography tandem mass spectrometry (nLC-MS/MS). Two time intervals were analysed; median 2–10 hours post-surgery (time A) and median 68–76 hours post-ICH onset (time B). Using 2-DE, we quantified 232 ± 31 different protein spots. Two proteins differed between the MD catheters at time A, and 12 proteins at time B (p < 0.05). Thirteen proteins were significantly altered between time A and time B in the SNX and seven proteins in the PHZ, respectively. Using nLC-MS/MS ca 800 proteins were identified out of which 76 were present in all samples. At time A one protein was upregulated and two downregulated, and at time B, seven proteins were upregulated, and four downregulated in the PHZ compared to the SNX. Microdialysis-based proteomics is feasible for study of secondary injury mechanisms and discovery of biomarkers after ICH.

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Section: Discussionmentioning

confidence: 94%

Dynamic protein changes in the perihaemorrhagic zone of Surgically Treated Intracerebral Haemorrhage Patients

Tobieson

Ghafouri

Zsigmond

et al. 2019

Sci Rep

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“…After awakening, the rats were assessed by masked observer through modified Neurological Severity Score (mNSS) ( Table 1). 18 The score (>8.5 points) was the success of the model. The control group rats were treated with 3 µL saline instead of collagenase and heparin sodium.…”

Section: Intracerebral Hemorrhage Modelmentioning

confidence: 99%

<p>Exercise training increases spatial memory via reducing contralateral hippocampal NMDAR subunits expression in intracerebral hemorrhage rats</p>

Zheng¹,

Zhang²,

Liu

et al. 2019

NDT

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The aim of this study was to explore the effect of exercise training on spatial memory in rats with intracerebral hemorrhage (ICH) and to analyze its related neurobiological mechanisms. Methods: A total of 26 Sprague-Dawley rats were randomly divided into 3 groups: exercise (EX) group undergoing exercise training after ICH, model (MD) group and sham-operated (SM) group. The ICH rats model were induced by infusion of type I collagenase into caudate nucleus of rats. Morris water maze (MWM) test was performed at the same time in three groups to evaluate spatial memory in rats. All rats were sacrificed for evaluation of expression of N-methyl-d-aspartate receptor 1 (NR1) and N-methyl-d-aspartate receptor 2B (NR2B) in the CA3 region of the hippocampus by Western blot. Results: MWM test results showed that the spatial memory of MD group was significantly decreased compared to that of SM operation group (P<0.05), while exercise training significantly improved the spatial memory of rats with cerebral hemorrhage (P<0.05). Western blot analysis showed that exercise training significantly decreased the expression of NR1 and NR2B in CA3 region of the contralateral hippocampus (P<0.05), but there was no significant difference between MD and SM groups (P>0.05). Conclusion: Exercise training improves the spatial memory in the rats with ICH via downregulating NR1 and NR2B expression in CA3 region of the contralateral hippocampus.

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“…Not only are primary hemorrhage-associated injuries of relevance, but secondary ICH-related injuries must also be taken into account [ 25 ]. For instance, the dysregulation of energy metabolism occurs in the setting of ICH, which could be a major contributor to secondary injury [ 26 , 27 , 28 ]. Furthermore, oxidative stress, inflammation, and iron toxicity also play important roles [ 2 , 29 ] [ 30 ].…”

Section: Discussionmentioning

confidence: 99%

MR-Imaging and Histopathological Diagnostic Work-Up of Patients with Spontaneous Lobar Intracerebral Hemorrhage: Results of an Institutional Prospective Registry Study

et al. 2021

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Intracerebral hemorrhage (ICH) is a frequently disabling or fatal disease. The localizationof ICH often allows an etiological association. However, in atypical/lobar ICH, the cause of bleedingis less obvious. Therefore, we present prospective histopathological and radiological studies whichwere conducted within the diagnostic workup to identify causes for lobar ICH other than hypertension.From 2016 to 2018, 198 patients with spontaneous, non-traumatic ICH requiring neurosurgicalmonitoring were enrolled in an institutional prospective patient registry. Patients with deep-seatedICH and/or hemorrhagically transformed cerebral infarcts were excluded from further analysis.Data to evaluate the source of bleeding based on histopathological and/or radiological workup wereprospectively evaluated and analyzed. After applying the inclusion criteria and excluding patientswith incomplete diagnostic workup, a total of 52 consecutive patients with lobar ICH were furtheranalyzed. Macrovascular disease was detected in 14 patients with lobar ICH (27%). In 11 patients,diagnostic workup identified cerebral amyloid angiopathy-related ICH (21%). In addition, five patientswith tumor-related ICH (10%) and six patients with ICH based on infectious pathologies(11%) were identified. In four patients, the cause of bleeding remained unknown despite extensivediagnostic workup (8%). The present prospective registry study demonstrates a higher probabilityto identify a cause of bleeding other than hypertension in patients with lobar ICH. Therefore, athorough diagnostic work-up in patients with ICH is essential to accelerate treatment and furtherimprove outcome or prevent rebleeding

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Quantitative proteomic analysis of intracerebral hemorrhage in rats with a focus on brain energy metabolism

Cited by 18 publications

References 82 publications

Dynamic protein changes in the perihaemorrhagic zone of Surgically Treated Intracerebral Haemorrhage Patients

Dynamic protein changes in the perihaemorrhagic zone of Surgically Treated Intracerebral Haemorrhage Patients

<p>Exercise training increases spatial memory via reducing contralateral hippocampal NMDAR subunits expression in intracerebral hemorrhage rats</p>

MR-Imaging and Histopathological Diagnostic Work-Up of Patients with Spontaneous Lobar Intracerebral Hemorrhage: Results of an Institutional Prospective Registry Study

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