Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Abstract

Exosomes derived from mesenchymal stem cells (MSCs) have been used for cancer treatment, however, an in‐depth analysis of the exosomal proteomes is lacking. In this manuscript, we use the diaPASEF (parallel accumulation serial fragmentation combined with the data‐independent acquisition) method to quantify exosomes derived from human umbilical cord mesenchymal stem cells (UCMSCs) and rat bone marrow stem cells (BMSCs), resulting in identification of 4200 human proteins and 5362 rat proteins. Comparison of human exosomal proteins and total cellular proteins reveals that some proteins exist in the exosomes exclusively that can be served as potential markers for exosomes. Quantitative proteomic analysis of exosomes from different passages of BMSCs shows that the proteins involved in TGF‐β signaling pathway are regulated in abundance, which could be markers for the therapeutic ability of BMSC exosomes. Collectively, the data presented by this study can be a resource for further study of exosome research.

Proteomic Analysis of Umbilical Cord Mesenchymal Stem Cell-Derived Extracellular Vesicles: A Systematic Review

“…The 11 studies reviewed were published between the year 2019 to 2022. Of the eleven studies, ten [39][40][41][42][43][44][45][46][47][48] had freshly isolated UCMSC, while only one study [49] had acquired UCMSC from a commercially available cell line. The majority of the studies assessed the trilineage differentiation capacity of their UCMSC except two [40,44].…”

“…For the MSC surface marker expression, many have screened at least one or more surface antigen markers for positive and/or negative markers. Only Xiao et al (2022) did not incorporate any of the surface marker analysis in their study design [47], for reasons that the authors did not present.…”

“…Meanwhile, study [40] used an EV protein marker from Category 2 of the same table. Only one study [47] had adopted at least one EV protein marker selected from Categories 1, 2, and 4. None of the studies were under Category 3, which represented the purity control based on recommendation in Table 3 of MISEV2018.…”

“…Most of the studies used a combination of basic culture media, which is the combination of Dulbecco's Modified Eagle Medium: Nutrient Mixture F12 (DMEM-F12) culture medium [40,41,43,47]. The rest of the studies [45,46,48] used single culture medium Low 3 summarize data for the UCMSC-EV production process.…”

ChaC1-based drug screenings identify a synergistic lethal effect of auranofin and proteasome inhibitors in hepatocellular carcinoma cells.

“…Proteomic study was performed as described 30 . The peptide samples were resolved in 2% ACN/0.1% FA for LC-MS analysis.…”