Quantitative proteomic analysis and identification of differentially expressed proteins involved in blast exposure-induced inflammatory response

Abstract

<jats:title>Abstract</jats:title> <jats:p>Background Previous studies found that blast injury caused a significant increased expression of interleukin-1, IL-6 and tumor necrosis factor, a significant decrease in the expression of IL-10, an increase in Evans blue leakage, and a significant increase in inflammatory cell infiltration in the lungs. However, the molecular characteristics of lung injury at different time points after blast exposure have not yet been reported. Therefore, in this study, tandem mass spectrometry (TMT) quantitative proteomics and bioinformatics analysis were used for the first time to gain a deeper understanding of the molecular mechanism of lung blast injury at different time points. Methods Fourty-eight male C57BL/6 mice were randomly divided into six groups: control, 12 h, 24 h, 48 h, 72 h and 1 w after low-intensity blast exposure. TMT quantitative proteomics and bioinformatics analysis were performed to analyze protein expression profiling in the lungs from control and blast-exposed mice, and differential protein expression was verified by Western blotting. Results The results demonstrated that blast exposure induced severe lung injury, leukocyte infiltration, and the production of inflammatory factors in mice. After analyzing the expression changes in global proteins and inflammation-related proteomes after blast exposure, the results showed that a total of 6,861 global proteins and 608 differentially expressed proteins were identified, of which 215, 128, 187, 232, and 65 proteins were identified at 12 h, 24 h, 48 h, 72 h, and 1 week after blast exposure, respectively. Moreover, blast exposure-induced 177 differentially expressed proteins were associated with inflammatory responses, which were enriched in the inflammatory response regulation, leukocyte transendothelial migration, phagocytosis, and immune response. Conclusions Therefore, blast exposure may induce early inflammatory response of lung tissue by regulating the expression of key proteins in the inflammatory process, suggesting that early inflammatory response may be the initiating factor of lung blast injury. These data can provide potential therapeutic candidates or approaches for the development of future treatment of lung blast injury.</jats:p>

An analysis of neurovascular disease markers in the hippocampus of Tupaia chinensis at different growth stages

“…Proteomics is a study of the dynamic changes of all proteins expressed by the genome of the cells of an organism in a specific time and space and analyzes the protein composition, the expression patterns, and the relationships among various components of the cell, enabling us to understand the unity of the structure and function of proteins, to further regulate the vital activities of living organisms, and to reveal the nature of life phenomena ( 18 ). The present study compared hippocampal protein expression profiles of T. chinensis at three different age groups from the perspective of bioinformatics to find proteins that manifest a significant change in the process of neural development and better explain the role of proteins in the development of the nervous system.…”