Quantitative proteome profiling of respiratory virus-infected lung epithelial cells

Diepen, Angela van; Brand, H. Kim; Sama, Iziah E.; Lambooy, Lambert H.J.; Heuvel, Lambert P. van den; Well, Leontine van der; Huynen, Martijn A.; Osterhaus, Albert D. M. E.; Andeweg, Arno C.; Hermans, Peter W. M.

doi:10.1016/j.jprot.2010.04.008

Cited by 48 publications

(69 citation statements)

References 69 publications

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“…However, for most cytokines, a significant component of this effect can still be detected after inactivation of the viral nucleic acid, and thus seems to be associated with a direct toxic effect of the capsid proteins. This observation is consistent with a number of previous studies, including a recent report showing minimal differences in direct comparisons between the proteomes from airway epithelial cells exposed to live or UV-inactivated RSV (49). More specifically, in lungs and in circulating leukocytes, UV-inactivated RSV still induces cytokines, such as IL-6 (50) and IL-8 (51), and chemokines, such as CCL6, CCL11, CCL22, and CXCL5 (52), and inhibits lymphocyte responses (53).…”

Section: Rsv Effects On Bmscssupporting

confidence: 93%

Respiratory Syncytial Virus Infection in Human Bone Marrow Stromal Cells

Rezaee¹,

Gibson²,

Piktel³

et al. 2011

Am J Respir Cell Mol Biol

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Respiratory syncytial virus (RSV) is the most common respiratory pathogen in infants and young children. The pathophysiology of this infection in the respiratory system has been studied extensively, but little is known about its consequences in other systems. We studied whether RSV infects human bone marrow stromal cells (BMSCs) in vitro and in vivo, and investigated whether and how this infection affects BMSC structure and hematopoietic support function. Primary human BMSCs were infected in vitro with recombinant RSV expressing green fluorescent protein. In addition, RNA from naive BMSCs was amplified by PCR, and the products were sequenced to confirm homology with the RSV genome. The BMSC cytoskeleton was visualized by immunostaining for actin. Finally, we analyzed infected BMSCs for the expression of multiple cytokines and chemokines, evaluated their hematopoietic support capacity, and measured their chemotactic activity for both lymphoid and myeloid cells. We found that BMSCs support RSV replication in vitro with efficiency that varies among cell lines derived from different donors; furthermore, RNA sequences homologous to the RSV genome were found in naive primary human BMSCs. RSV infection disrupted cytoskeletal actin microfilaments, altered cytokine/chemokine expression patterns, decreased the ability of BMSCs to support B cell maturation, and modulated local chemotaxis. Our data indicate that RSV infects human BMSCs in vitro, and this infection has important structural and functional consequences that might affect hematopoietic and immune functions. Furthermore, we have amplified viral RNA from naive primary BMSCs, suggesting that in vivo these cells provide RSV with an extrapulmonary target.

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Section: Rsv Effects On Bmscssupporting

confidence: 93%

Respiratory Syncytial Virus Infection in Human Bone Marrow Stromal Cells

Rezaee¹,

Gibson²,

Piktel³

et al. 2011

Am J Respir Cell Mol Biol

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“…Various proteomic workflows have been exploited in endeavors to define the impacts of the infection of cultured cells with hRSV (23)(24)(25)(26)(27)(28)(29). These varied in analytical complexity from 2D-gel-based methods (23)(24)(25)29) through to more complex HPLC-MS of samples isolated by other electrophoretic methods (24, 26 -28).…”

Section: Discussionmentioning

confidence: 99%

“…Although this may be beneficial to the virus, a better understanding of how hRSV impairs host antiviral responses may also be the basis for design of effective vaccines and/or therapeutic strategies. Accordingly, a variety of studies have been conducted to define host cell responses to hRSV infection at both the transcriptional (18 -22) and proteomic (23)(24)(25)(26)(27)(28)(29) levels. This has included studies with forms of hRSV lacking genes that encode proteins known to impair host innate antiviral responses, such as the nonstructural protein1 (NS1) (24).…”

mentioning

confidence: 99%

“…For instance, the use of fractionation of cells into cytoplasmic and nuclear fractions (23,(25)(26)(27)) is likely to have perturbed the ability to reliably quantify protein abundance changes at a global cellular level. Some of these studies may have been limited because of the lack of penetrating coverage of the proteomes using twodimensional (2D)-gel-based protocols (23)(24)(25)29), with (23,25) and without (24,29) subcellular fractionation steps. In some instances, proteome coverage may have been limited from an analytical technology standpoint (23)(24)(25)(26)(27)(28)(29).…”

mentioning

confidence: 99%

“…Some of these studies may have been limited because of the lack of penetrating coverage of the proteomes using twodimensional (2D)-gel-based protocols (23)(24)(25)29), with (23,25) and without (24,29) subcellular fractionation steps. In some instances, proteome coverage may have been limited from an analytical technology standpoint (23)(24)(25)(26)(27)(28)(29). Finally, the reliability of some studies is questionable because of a lack of any biological or technical replicates (26 -28).…”

mentioning

confidence: 99%

See 2 more Smart Citations

A Comprehensive Proteomic View of Responses of A549 Type II Alveolar Epithelial Cells to Human Respiratory Syncytial Virus Infection

Dave

Norris

Bukreyev

et al. 2014

Molecular & Cellular Proteomics

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Mass Spectrometry in Virological Sciences

Milewska

Ner‐Kluza

Dąbrowska

et al. 2019

Mass Spectrometry Reviews

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Virology, as a branch of the life sciences, discovered mass spectrometry (MS) to be the pivotal tool around two decades ago. The technique unveiled the complex network of interactions between the living world of pro-and eukaryotes and viruses, which delivered "a piece of bad news wrapped in protein" as defined by Peter Medawar, Nobel Prize Laureate, in 1960. However, MS is constantly evolving, and novel approaches allow for a better understanding of interactions in this micro-and nanoworld. Currently, we can investigate the interplay between the virus and the cell by analyzing proteomes, interactomes, virus-cell interactions, and search for the compounds that build viral structures. In addition, by using MS, it is possible to look at the cell from the broader perspective and determine the role of viral infection on the scale of the organism, for example, monitoring the crosstalk between infected tissues and the immune system. In such a way, MS became one of the major tools for the modern virology, allowing us to see the infection in the context of the whole cell or the organism.

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Quantitative proteome profiling of respiratory virus-infected lung epithelial cells

Cited by 48 publications

References 69 publications

Respiratory Syncytial Virus Infection in Human Bone Marrow Stromal Cells

Respiratory Syncytial Virus Infection in Human Bone Marrow Stromal Cells

A Comprehensive Proteomic View of Responses of A549 Type II Alveolar Epithelial Cells to Human Respiratory Syncytial Virus Infection

Mass Spectrometry in Virological Sciences

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