Quantitative phase microscopy of red blood cells during planar trapping and propulsion

Ahmad, Azeem; Dubey, Vishesh; Singh, Vijay Raj; Tinguely, Jean-Claude; Øie, Cristina Ionica; Wolfson, Deanna L.; Mehta, Dalip Singh; So, Peter T. C.; Ahluwalia, Balpreet Singh

doi:10.1039/c8lc00356d

Cited by 28 publications

(32 citation statements)

References 52 publications

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“…In particular, biomechanical markers of sickle red blood cells can be analyzed on a fly in a cytometric setting. 7 Potential applications also include cancer cell nuclear mechanics as a possible marker for metastasis.…”

Section: Novel Methods In Elastographymentioning

confidence: 99%

Optical elastography and tissue biomechanics

2019

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Mechanical forces play an important role in the behavior and development of biological systems and disease at all spatial scales, from cells and their constituents to tissues and organs. Such forces have a profound influence on the health, structural integrity, and normal function of cells and organs. Accurate knowledge of cell and tissue biomechanical properties is essential to map the distribution of forces and mechanical cues in biological systems. Cell and tissue biomechanical properties are also known to be important on their own as indicators of health or disease states. Hence, optical elastography and biomechanics methods can aid in the understanding and clinical diagnosis of a wide variety of diseases. We provide a brief overview and highlight of the Optical Elastography and Tissue Biomechanics VI conference, which took place in San Francisco, February 2 and 3, 2019, as a part of Photonics West symposium.

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Section: Novel Methods In Elastographymentioning

confidence: 99%

Optical elastography and tissue biomechanics

2019

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“…The results of computer simulation of moving erythrocytes in an evanescent field will now be supported experimentally. A blood plasma 30 is prepared by centrifugation of fresh venous blood at room temperature. Initially, a whole blood (1500 g) is centrifuged during 10 min, which results in separation of sedimented erythrocytic masses from plasma.…”

Section: Experimental Demonstration Of Transversal Motion Of Erythrocmentioning

confidence: 99%

Peculiarities of control of erythrocytes moving in an evanescent field

et al. 2019

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An investigation of the influence of an evanescent wave on the dynamics of motion of erythrocytes in blood plasma is presented. Computer simulation of erythrocytes moving in an evanescent field and experimental demonstration of the forecasted motion substantiate the possibility for control of position of red blood cells in a solution. The range of velocities of transversal motion of erythrocytes due to the action of the optical force of the generated evanescent field is determined as a function of the angle of illumination of a cell by a linearly polarized wave with the azimuth of polarization 45 deg.

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“…To measure the visibility/contrast of the interferograms Fourier transform algorithm is employed. The 2D intensity distribution of the interferograms is given by the following expression 20,21 :…”

Section: Visibility/contrast Measurement Of the Interferogrammentioning

confidence: 99%

Relationship between the source size at the diffuser plane and the longitudinal spatial coherence function of the optical coherence microscopy system

Usmani¹,

Ahmad²,

Joshi³

et al. 2019

J. Opt. Soc. Am. A

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Coherence properties and wavelength of light sources are indispensable for optical coherence microscopy/tomography (OCM/T) as they greatly influence the signal to noise ratio, axial resolution, and penetration depth of the system. In the present letter, we investigated the longitudinal spatial coherence properties of pseudo/thermal light source (PTS) as a function of spot size at the diffuser plane, which is controlled by translating microscope objective lens towards or away from the diffuser plane. The axial resolution of PTS is found to be maximum ~ 13 µm for the beam spot size of 3.5 mm at the diffuser plane. The change in the axial resolution of the system as the spot size is increased at the diffuser plane is further confirmed by performing experiments on standard gauge blocks of height difference of 15 µm. Thus, by appropriately choosing the beam spot size at the diffuser plane, any monochromatic laser light source depending on the biological window can be utilized to obtain high axial-resolution with large penetration depth and speckle free tomographic images of multilayered biological specimens irrespective of the source's temporal coherence length. In addition, PTS could be an attractive alternative light source for achieving high axial-resolution without needing chromatic aberration corrected optics and dispersion-compensation mechanism unlike conventional setups.Recently, a number of studies have been put forward to synthesize a spatially extended and temporally coherent light source named pseudo-thermal light source (PTS) to overcome

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Quantitative phase microscopy of red blood cells during planar trapping and propulsion

Abstract: Here, we have combined quantitative phase microscopy and waveguide trapping techniques to study changes in RBC morphology during planar trapping and transportation.

Cited by 28 publications

References 52 publications

Optical elastography and tissue biomechanics

Optical elastography and tissue biomechanics

Peculiarities of control of erythrocytes moving in an evanescent field

Relationship between the source size at the diffuser plane and the longitudinal spatial coherence function of the optical coherence microscopy system

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