Quantitative phase-contrast imaging with compact digital holographic microscope employing Lloyd’s mirror

Chhaniwal, Vani K.; Singh, Amardeep; Leitgeb, Rainer A.; Javidi, Bahram; Anand, Arun

doi:10.1364/ol.37.005127

Cited by 125 publications

(56 citation statements)

References 19 publications

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“…QPI employing Michelson interferometer is suitable for the reflection geometry [15,16]. However, the Michelson or Mach-Zehnder interferometry suffers from time-varying phase noise due to vibration, temperature gradient, and air flow, which deteriorate the stability of QPI measurements.…”

Section: Principles Of Qpimentioning

confidence: 99%

Quantitative Phase Imaging Techniques for the Study of Cell Pathophysiology: From Principles to Applications

Lee

Jung

et al. 2013

Sensors

460

283

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A cellular-level study of the pathophysiology is crucial for understanding the mechanisms behind human diseases. Recent advances in quantitative phase imaging (QPI) techniques show promises for the cellular-level understanding of the pathophysiology of diseases. To provide important insight on how the QPI techniques potentially improve the study of cell pathophysiology, here we present the principles of QPI and highlight some of the recent applications of QPI ranging from cell homeostasis to infectious diseases and cancer.

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Section: Principles Of Qpimentioning

confidence: 99%

Quantitative Phase Imaging Techniques for the Study of Cell Pathophysiology: From Principles to Applications

Lee

Jung

et al. 2013

Sensors

460

283

View full text Add to dashboard Cite

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“…For dynamic imaging of cells, microscope setups with sub-nanometer temporal stability is required. Such microscopes could be implemented with common path self-referencing geometry and can image sub-nanometer oscillations of cell membranes [8][9][10]. The next step is to combine the static as well as dynamic parameters provided by this type of digital holographic microscopes for identification of cells.…”

Section: Discussionmentioning

confidence: 99%

“…Digital holographic microscopy is such a technique, providing a means for effective three-dimensional imaging of micro-objects [1][2][3][4][5][6][7][8][9][10]. Digital holograms directly provide the phase information of the object, from which its thickness profile can be reconstructed [6,7].…”

Section: Introductionmentioning

confidence: 99%

Identification of malaria infected red blood samples by digital holographic quantitative phase microscope

Patel

Chhaniwal

Javidi³

et al. 2015

SPIE Proceedings

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Development of devices for automatic identification of diseases is desired especially in developing countries. In the case of malaria, even today the gold standard is the inspection of chemically treated blood smears through a microscope. This requires a trained technician/microscopist to identify the cells in the field of view, with which the labeling chemicals gets attached. Bright field microscopes provide only low contrast 2D images of red blood cells and cell thickness distribution cannot be obtained. Quantitative phase contrast microscopes can provide both intensity and phase profiles of the cells under study. The phase information can be used to determine thickness profile of the cell. Since cell morphology is available, many parameters pertaining to the 3D shape of the cell can be computed. These parameters in turn could be used to decide about the state of health of the cell leading to disease diagnosis. Here the investigations done on digital holographic microscope, which provides quantitative phase images, for comparison of parameters obtained from the 3D shape profile of objects leading to identification of diseased samples is described.

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“…In DHM, the introduction of a separate reference beam entails the use of additional optical elements and may also result in different optical pathlength changes in the two arms of the interferometer, which leads to higher noises. These noises can be greatly reduced by the use of a common-path geometry [4]. In this paper, based on the combination of the method presented in [4] and a Mack-Zender geometry, we utilized a simple and common path DHM setup, in which both of the object and the reference beams are achieved of an individual wavefront of light transmitted through the phase sample under microscopic study.…”

Section: Introductionmentioning

confidence: 99%

“…These noises can be greatly reduced by the use of a common-path geometry [4]. In this paper, based on the combination of the method presented in [4] and a Mack-Zender geometry, we utilized a simple and common path DHM setup, in which both of the object and the reference beams are achieved of an individual wavefront of light transmitted through the phase sample under microscopic study. The developed technique is common path, compact, and highly stable.…”

Section: Introductionmentioning

confidence: 99%

Detection of the effect of nanoparticles on myelin figures growth using a compact digital holographic microscope

et al. 2013

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Digital holographic microscopy (DHM) is an effective and non-destructive technique for quantitative phase contrast imaging of biological samples and living organelles. In this paper, using a simple and stable common-path DHM setup we study lipid bilayer dynamics and detect their morphological changes. Stacks of lipid amphiphilic molecules in excess water and at the presence of an external stimulus, stress, or force have great capability for the formation of multilamellar cylindrical tubes that are called myelin figures(MFs). MFs can be found in various healthy and diseased living cells and their formation and dynamics in various conditions involve mysterious configurations that have been of high interest. We utilized nanoparticles solved in water with different concentrations as an external stimulus for MFs of POPC lipid. The nanoparticles are injected into the sample container via a microinjection pump in a constant rate and MFs growth rate and their volume changes are measured by a compact digital holographic system. The setup is based on a binocular conventional microscope making the setup very stable against vibrations and noises. The recorded holograms are then computationally reconstructed. The measurements and investigations are performed by analyzing the reconstruction process. We showed that nanoparticles increase the growth rate of MFs during the first few seconds. However, after few seconds, the growth rate does not alter significantly comparing to the absence of nanoparticles.

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Quantitative phase-contrast imaging with compact digital holographic microscope employing Lloyd’s mirror

Cited by 125 publications

References 19 publications

Quantitative Phase Imaging Techniques for the Study of Cell Pathophysiology: From Principles to Applications

Quantitative Phase Imaging Techniques for the Study of Cell Pathophysiology: From Principles to Applications

Identification of malaria infected red blood samples by digital holographic quantitative phase microscope

Detection of the effect of nanoparticles on myelin figures growth using a compact digital holographic microscope

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