Quantitative paper spray mass spectrometry analysis of drugs of abuse

Su, Yu‐Nung; He, Wei; Liu, Jiangjiang; Pu, Weihua; Cooks, R. Graham; Ouyang, Zheng

doi:10.1039/c3an00934c

Cited by 120 publications

(91 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Extraction/spray solvent Some spray solvents commonly found in the literature include 90:10:0.01 methanol:water:acetic acid [8], 90:10 acetonitrile:water [5,9], and mixtures of methanol and chloroform [4,10]. Acetic or formic acid is often added in the spray solvent to encourage analyte protonation and to improve spray stability.…”

Section: Sample Considerationsmentioning

confidence: 99%

“…Acetonitrile based extraction solvents are excellent solvents for plasma and urine samples, but do not penetrate dried blood spots particularly well in our experience. On the other hand, other researchers have reported the use of acetonitrile based solvents on dried blood spots with good results [9]. High proportions of water are generally not used for bioanalysis by paper spray MS.…”

Section: Sample Considerationsmentioning

confidence: 99%

See 1 more Smart Citation

Analysis of Biofluids by Paper Spray MS: Advances and Challenges

2016

View full text Add to dashboard Cite

Paper spray mass spectrometry (MS) is part of a cohort of ambient ionization or direct analysis methods that seek to analyze complex samples without prior sample preparation. Extraction and electrospray ionization occur directly from the paper substrate upon which a dried matrix spot is stored. Paper spray MS is capable of detecting drugs directly from dried blood, plasma, and urine spots at the low ng/mL to pg/mL levels without sample preparation. No front end separation is performed, so tandem mass spectrometry (MS/MS) or high resolution MS is required. Here, we discuss paper spray methodology, give a comprehensive literature review of the use of paper spray MS for bioanalysis, discuss technological advancements and variations on this technique, and discuss some of its limitations. _________________________________________________________________________________ AbstractPaper spray mass spectrometry (MS) is part of a cohort of ambient ionization or direct analysis methods that seek to analyze complex samples without prior sample preparation. Extraction and electrospray ionization occur directly from the paper substrate upon which a dried matrix spot is stored. Paper spray MS is capable of detecting drugs directly from dried blood, plasma, and urine spots at the low ng/mL to pg/mL levels without sample preparation. No front end separation is performed, so tandem mass spectrometry (MS/MS) or high resolution MS is required. Here, we discuss paper spray methodology, give a comprehensive literature review of the use of paper spray MS for bioanalysis, discuss technological advancements and variations on this technique, and discuss some of its limitations.

show abstract

Section: Sample Considerationsmentioning

confidence: 99%

Section: Sample Considerationsmentioning

confidence: 99%

Analysis of Biofluids by Paper Spray MS: Advances and Challenges

2016

View full text Add to dashboard Cite

show abstract

“…Paper spray is a member of this growing cohort of ambient ionization or direct analysis methods that was conceived because of the growing interest in storing and transporting blood samples as dried spots. One of the major applications of paper spray mass spectrometry has been screening and quantitative analysis of drugs and other small molecules from biofluids such as blood, urine, and plasma [3][4][5][6][7][8][9][10][11][12][13][14].…”

mentioning

confidence: 99%

Ionization Suppression and Recovery in Direct Biofluid Analysis Using Paper Spray Mass Spectrometry

Vega

Spence

Zhang

et al. 2016

J. Am. Soc. Mass Spectrom.

View full text Add to dashboard Cite

Abstract. Paper spray mass spectrometry is a method for the direct analysis of biofluid samples in which extraction of analytes from dried biofluid spots and electrospray ionization occur from the paper on which the dried sample is stored. We examined matrix effects in the analysis of small molecule drugs from urine, plasma, and whole blood. The general method was to spike stable isotope labeled analogs of each analyte into the spray solvent, while the analyte itself was in the dried biofluid. Intensity of the labeled analog is proportional to ionization efficiency, whereas the ratio of the analyte intensity to the labeled analog in the spray solvent is proportional to recovery. Ion suppression and recovery were found to be compound-and matrix-dependent. Highest levels of ion suppression were obtained for poor ionizers (e.g., analytes lacking basic aliphatic amine groups) in urine and approached -90%. Ion suppression was much lower or even absent for good ionizers (analytes with aliphatic amines) in dried blood spots. Recovery was generally highest in urine and lowest in blood. We also examined the effect of two experimental parameters on ion suppression and recovery: the spray solvent and the sample position (how far away from the paper tip the dried sample was spotted). Finally, the change in ion suppression and analyte elution as a function of time was examined by carrying out a paper spray analysis of dried plasma spots for 5 min by continually replenishing the spray solvent.

show abstract

“…is technique was demonstrated to be able to not only analyze complex samples, but also perform quantitative analysis. 29,30,[32][33][34][35] Paper spray was successfully applied in direct detection and quantitation of therapeutic drugs in blood and tissue samples, providing a rapid and reliable method in therapeutic monitoring. 29,30,[32][33][34] Very recently, this technique was utilized in determination of drugs-ofabuse in biological uids 35) and trace amount of illicit drugs on a solid surface (desktop).…”

Section: Paper Spraymentioning

confidence: 99%

“…29,30,[32][33][34][35] Paper spray was successfully applied in direct detection and quantitation of therapeutic drugs in blood and tissue samples, providing a rapid and reliable method in therapeutic monitoring. 29,30,[32][33][34] Very recently, this technique was utilized in determination of drugs-ofabuse in biological uids 35) and trace amount of illicit drugs on a solid surface (desktop). 30) Furthermore, the use of paper spray in food analysis, e.g., determination of Sudan azo-dyes in chilli pepper, 36) detection of agrochemicals on fruit peels, 30) chemical ngerprint analysis of Bansha herbal tea 37) and determination of nonosteroidal anti-in ammatory drugs in olive oil, 38) were demonstrated.…”

Section: Paper Spraymentioning

confidence: 99%

Electrospray Ionization on Solid Substrates

Yao

2014

Mass Spectrometry

View full text Add to dashboard Cite

Development of electrospray ionization on solid substrates (solid-substrate ESI) avoids the clogging problem encountered in conventional capillary-based ESI, allows more convenient sampling and permits new applications. So far, solid-substrate ESI with various materials, e.g., metals, paper, wood, bers and biological tissue, has been developed, and applications ranging from analysis of pure compounds to complex mixtures as well as in vivo study were demonstrated. Particularly, the capability of solid-substrate ESI in direct analysis of complex samples, e.g., biological uids and foods, has signi cantly facilitated mass spectrometric analysis in real-life applications and led to increasingly important roles of these techniques nowadays. In this review, various solid-substrate ESI techniques and their applications are summarized and the prospects in this eld are discussed.Please cite this article as: P.-K. So, et al., Electrospray Ionization on Solid Substrates, Mass Spectrom (Tokyo) 2014; 3(2): S0028;

show abstract

Quantitative paper spray mass spectrometry analysis of drugs of abuse

Abstract: An ambient method for rapid monitoring and quantitation of drugs of abuse in dried blood spots was developed using paper spray tandem mass spectrometry (PS-MS).

Cited by 120 publications

References 28 publications

Analysis of Biofluids by Paper Spray MS: Advances and Challenges

Analysis of Biofluids by Paper Spray MS: Advances and Challenges

Ionization Suppression and Recovery in Direct Biofluid Analysis Using Paper Spray Mass Spectrometry

Electrospray Ionization on Solid Substrates

Contact Info

Product

Resources

About