Quantitative NMR-Based Metabolomics on Tissue Biomarkers and Its Translation into In Vivo Magnetic Resonance Spectroscopy

Serkova, Natalie J.; Davis, Dale; Steiner, Jenna; Agarwal, Rajesh

doi:10.1007/978-1-4939-9236-2_23

Cited by 8 publications

(6 citation statements)

References 30 publications

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“…have successfully shown high throughput chemical data ( Table 1 ). In turn, NMR sensitivity has been improved through the progress in hardware, magnetic field strength, probes design and pulse sequence programs ( Serkova et al, 2019 ).…”

Section: Nuclear Magnetic Resonance (Nmr) Spectroscopymentioning

confidence: 99%

NMR spectroscopy spotlighting immunogenicity induced by COVID-19 vaccination to mitigate future health concerns

Ali

Nedvědová

Badshah

et al. 2022

Current Research in Immunology

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Section: Nuclear Magnetic Resonance (Nmr) Spectroscopymentioning

confidence: 99%

NMR spectroscopy spotlighting immunogenicity induced by COVID-19 vaccination to mitigate future health concerns

Ali

Nedvědová

Badshah

et al. 2022

Current Research in Immunology

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“…This discrepancy in TSP levels would thus result in the overestimation of metabolites by MAS MRS. Water resonance has also been utilized as an internal standard in MAS MRS experiments [121]; however, water content is likely to vary between tissues with heavy fat content, such as breast tissues from cancer patients, and thus, can be an inconsistent reference for MAS MRS samples. External standards have also been utilized in MAS MRS as a method of metabolite quantification [122]. As in solution state NMR, ERETIC has demonstrated advantages in MAS MRS applications over traditional internal and external reference standards [16,119,123].…”

Section: Quantification By Solid State Nmr Spectroscopymentioning

confidence: 99%

Quantitative NMR-Based Biomedical Metabolomics: Current Status and Applications

Crook

Powers

2020

Molecules

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Nuclear Magnetic Resonance (NMR) spectroscopy is a quantitative analytical tool commonly utilized for metabolomics analysis. Quantitative NMR (qNMR) is a field of NMR spectroscopy dedicated to the measurement of analytes through signal intensity and its linear relationship with analyte concentration. Metabolomics-based NMR exploits this quantitative relationship to identify and measure biomarkers within complex biological samples such as serum, plasma, and urine. In this review of quantitative NMR-based metabolomics, the advancements and limitations of current techniques for metabolite quantification will be evaluated as well as the applications of qNMR in biomedical metabolomics. While qNMR is limited by sensitivity and dynamic range, the simple method development, minimal sample derivatization, and the simultaneous qualitative and quantitative information provide a unique landscape for biomedical metabolomics, which is not available to other techniques. Furthermore, the non-destructive nature of NMR-based metabolomics allows for multidimensional analysis of biomarkers that facilitates unambiguous assignment and quantification of metabolites in complex biofluids.

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“…Proton NMR spectra were acquired using a 400 MHz NMR spectrometer (Bruker, Ettlingen, Germany). The samples were detected in a standard 1-dimension (1-D) pulse sequence as standard Bruker pulse program or zg30 pulse sequence at a temperature of 298 K in 32 scans with spectral width of 20 ppm and 1 s relaxation decay (22,23). The chemical shift referencing, baseline correction and phasing were conducted in TopSpin (Bruker, Germany).…”

Section: Extraction Of Metabolites From Hamster Liver Tissuesmentioning

confidence: 99%

Metabolic Profiling of Praziquantel-mediated Prevention ofOpisthorchis viverrini-induced Cholangiocyte Transformation in the Hamster Model of Cholangiocarcinoma

Prommajun

Phetcharaburanin

Namwat

et al. 2021

Cancer Genomics Proteomics

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Background: Opisthorchis viverrini (Ov) infectioninduced cholangiocarcinoma (CCA) is a major public health problem in northeastern Thailand. Praziquantel was shown to prevent CCA development in an Ov-infected hamster model; however, the molecular mechanism remains unknown. Materials and Methods: In this study, we used a hamster model with Ov and N-nitrosodimethylamine-induced CCA to study the mechanisms of praziquantel action. The liver tissues from the hamsters with and without praziquantel treatment were analyzed using 1 H nuclear magnetic resonance spectroscopy. Results: A total of 14 metabolites were found to be significantly different between the two groups. Furthermore, the combination of acetate, inosine and sarcosine was shown to exert an anti-inflammatory effect through interleukin-6 inhibition in a macrophage cell line, suggesting a mechanism by which praziquantel may prevent inflammation caused by Ov, cholangiocyte transformation and further CCA develpoment. Conclusion: These findings might avail the development of a preventive strategy for CCA in high-risk populations.Cholangiocarcinoma (CCA), a malignancy of bile duct epithelial cells, is a major public health problem in Northeast Thailand, which has the highest incidence in the world of this disease (1). A parasitic infection caused by Opisthorchis viverrini (Ov), a liver fluke, was identified as a cause of CCA in 1994 and is associated with CCA development (1). Upon liver fluke infection, CCA genesis is hypothesized to be initially induced via multiple mechanistic pathways, including mechanical damage caused by the fluke suckers, fluke toxic secretory products, and the host immunopathological response, leading to chronic inflammation (2). Moreover, the overproduction of reactive oxygen species and reactive nitrogen species from chronic inflammation results in tissue injury associated with CCA initiation and progression (3). In accordance with the hepatic changes first reported in 1978 in an Ov-infected hamster model, hyperplasia, adenomatous formations and periductal and portal scarring of the bile duct epithelium were also observed at 22 weeks post infection, although bile duct carcinoma was not seen at that time (4). In addition, a previous study showed that alone, Nnitrosodimethylamine (NDMA), a potential carcinogen, did not induce tumor formation in hamsters, but together with Ov infection, CCA was observed in a number of animals (5). Furthermore, histological changes, such as inflammatory reactions, bile duct proliferation, periductal fibrosis and cholangiocarcinoma lesions, were all observed in this cotreatment group (5). At the gene-expression level, we have shown a single down-regulated and 23 up-regulated give transcripts that were involved in Ov-induced CCA development (6). The up-regulated genes include signal 29 This article is freely accessible online.

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Quantitative NMR-Based Metabolomics on Tissue Biomarkers and Its Translation into In Vivo Magnetic Resonance Spectroscopy

Cited by 8 publications

References 30 publications

NMR spectroscopy spotlighting immunogenicity induced by COVID-19 vaccination to mitigate future health concerns

NMR spectroscopy spotlighting immunogenicity induced by COVID-19 vaccination to mitigate future health concerns

Quantitative NMR-Based Biomedical Metabolomics: Current Status and Applications

Metabolic Profiling of Praziquantel-mediated Prevention ofOpisthorchis viverrini-induced Cholangiocyte Transformation in the Hamster Model of Cholangiocarcinoma

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