Quantitative NMR analysis of <scp>L</scp>‐Dopa in seeds from two varieties of <scp><i>Mucuna pruriens</i></scp>

Fernández-Pastor, Ignacio; Luque-Muñoz, Antonio; Rivas, Francisco; Medina-O'Donnell, Marta; Martı́nez, Antonio; Gonzalez-Maldonado, R; Haı̈dour, Ali; Parra, Andrés

doi:10.1002/pca.2793

Cited by 18 publications

(12 citation statements)

References 17 publications

(28 reference statements)

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“… 14 , 15 , 16 Based on phytochemical profiles, the L-3,4 dihydroxyphenyl alanine ( l -DOPA) is demonstrated to be a major active substance found in the seeds of M. pruriens with high antioxidant capacity. 17 , 18 , 19 Moreover, M. pruriens seed has been proven to improve semen quality and antioxidant enzymes in seminal plasma of infertile men. 5 In rodents, M. pruriens could reduce the damages of sperm DNA and testicular mitochondria.…”

Section: Introductionmentioning

confidence: 99%

Protective effects of Thai Mucuna pruriens (L.) DC. var. pruriens seeds on sexual behaviors and essential reproductive markers in chronic unpredictable mild stress mice

Choowong-In

Sattayasai

Boonchoong

et al. 2022

Journal of Traditional and Complementary Medicine

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Section: Introductionmentioning

confidence: 99%

Protective effects of Thai Mucuna pruriens (L.) DC. var. pruriens seeds on sexual behaviors and essential reproductive markers in chronic unpredictable mild stress mice

Choowong-In

Sattayasai

Boonchoong

et al. 2022

Journal of Traditional and Complementary Medicine

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“…which were extracted from cloves using 1 H-NMR and 13 C-NMR (Thirukumaran et al, 2014), pyrazole-based ligand analysis (Bouroumane et al, 2021), and others. In addition to qualitative use, NMR spectroscopy can also be used for quantitative analysis purposes (Fernandez-Pastor et al (2019)). However, the reading and interpretation of the 1 H-NMR and 13 C-NMR spectra were not described in detail.…”

Section: Introductionmentioning

confidence: 99%

How to Read and Interpret 1H-NMR and 13C-NMR Spectrums

Gunawan

Nandiyanto

2021

Indonesian J. Sci. Technol

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Nuclear magnetic resonance spectroscopy or NMR is a chemical instrument that can be used to evaluate the structure of a chemical compound other than FTIR, GC-MS, and HPLC. NMR spectroscopy commonly used for compound analysis is 1H-NMR and 13C-NMR. Techniques can be used to determine the structure conformation, the number of protons, and the number of carbons in the structure of a chemical compound. So far, there have been many publications related to the use of this spectroscopic technique. However, the steps in reading and interpreting the spectra of both 1H-NMR and 13C-NMR are not described in detail. Thus, in this paper, we described the steps in reading and interpreting the 1H-NMR and 13C-NMR spectra based on the level of difficulties: (1) simple compounds, (2) fairly complex compounds, (3) more complex compounds, and (4) very complex compounds.

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“…Fernandez-Pastor et al [ 29 ] used the internal standard method to quantify L-Dopa in M. Pruriens seeds, and an NMR calibration curve was obtained to evaluate the accuracy and reproducibility of the method. The results confirm that the internal standard method has reliable accuracy and reproducibility and that a standard curve is not needed for the quantification.…”

Section: Overview Of Qnmrmentioning

confidence: 99%

Research Progress of NMR in Natural Product Quantification

Wang

You

et al. 2021

Molecules

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In the fields of medicine and health, traditional high-performance liquid chromatography or UV-visible spectrophotometry is generally used for substance quantification. However, over time, nuclear magnetic resonance spectroscopy (NMR) has gradually become more mature. Nuclear magnetic resonance spectroscopy has certain advantages in the quantitative analysis of substances, such as being nondestructive, having a high flux and short analysis time. Nuclear magnetic resonance spectroscopy has been included in the pharmacopoeiae of various countries. In this paper, the principle of nuclear magnetic resonance spectroscopy and the recent progress in the quantitative study of natural products by NMR are reviewed, and its application in the quantitative study of natural products is proposed. At the same time, the problems of using NMR alone to quantify natural products are summarized and corresponding suggestions are put forward.

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Quantitative NMR analysis of L‐Dopa in seeds from two varieties of Mucuna pruriens

Abstract: The statistical analysis confirmed the accuracy and reproducibility of this single point qNMR method (internal standard) for determining L-Dopa, as well as other commercial preparations of this species, without performing an NMR calibration curve.

Cited by 18 publications

References 17 publications

Protective effects of Thai Mucuna pruriens (L.) DC. var. pruriens seeds on sexual behaviors and essential reproductive markers in chronic unpredictable mild stress mice

Protective effects of Thai Mucuna pruriens (L.) DC. var. pruriens seeds on sexual behaviors and essential reproductive markers in chronic unpredictable mild stress mice

How to Read and Interpret 1H-NMR and 13C-NMR Spectrums

Research Progress of NMR in Natural Product Quantification

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